Viroid-like RNA was isolated from some samples of grapevine leaves ( L.). When inoculated to cucumber, this RNA induced symptoms that were identical to those induced by hop stunt viroid (HSV). It had a molecular size similar to that of HSV, and hybridized with a P-labelled DNA probe for the cucumber isolate of HSV (HSV-c, pCP-55). This hybridization suggests high (> 55%) sequence homology with HSV. The HSV-related sequence was detected not only in the infected cucumber plants but also directly in RNA extracted from grapevine plants. The result indicates that viroid-like RNA, closely related to HSV, is present in grapevines in Japan.

Keyword(s): grapevine , hop stunt viroid and viroid

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