1887

Abstract

SUMMARY

The survival of airborne human coronavirus 229E (HCV/229E) was studied under different conditions of temperature (20 ± 1 °C and 6 ± 1 °C) and low (30 ± 5%), medium (50 ± 5%) or high (80 ± 5%) relative humidities (RH). At 20 ± 1 °C, aerosolized HCV/229E was found to survive best at 50% RH with a half-life of 67.33 ± 8.24 h while at 30% RH the virus half-life was 26.76 ± 6.21 h. At 50% RH nearly 20% infectious virus was still detectable at 6 days. High RH at 20 ± 1 °C, on the other hand, was found to be the least favourable to the survival of aerosolized virus and under these conditions the virus half-life was only about 3 h; no virus could be detected after 24 h in aerosol. At 6 ± 1 °C, in either 50% or 30% RH conditions, the survival of HCV/229E was significantly enhanced, with the decay pattern essentially similar to that seen at 20 ± 1 °C. At low temperature and high RH (80%), however, the survival pattern was completely reversed, with the HCV/229E half-life increasing to 86.01 ± 5.28 h, nearly 30 times that found at 20 ± 1 °C and high RH. Although optimal survival at 6 °C still occurred at 50% RH, the pronounced stabilizing effect of low temperature on the survival of HCV/229E at high RH indicates that the role of the environment on the survival of viruses in air may be more complex and significant than previously thought.

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1985-12-01
2024-03-19
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References

  1. Akers T. G. 1973; Some aspects of the airborne inactivation of viruses. In Airborne Transmission and Airborne Infection pp 73–81 Edited by Hers J. F. P., Winkler K. C. New York: John Wiley & Sons;
    [Google Scholar]
  2. Beard C. W., Easterday B. C. 1965; An aerosol apparatus for the exposure of large and small animals: description and operating characteristics. American Journal of Veterinary Research 26:174–182
    [Google Scholar]
  3. Caul E. O., Clarke S. K. R. 1975; Coronavirus propagated from a patient with non-bacterial gastroenteritis. Lancet ii:953–954
    [Google Scholar]
  4. Caul E. O., Egglestone S. I. 1982; Coronaviruses in humans. In Virus Infections of The Gastrointestinal Tract pp 179–193 Edited by Tyrrell D. A. J., Kapikian A. Z. New York: Marcel Dekker;
    [Google Scholar]
  5. Cavallaro J. J., Monto A. S. 1970; Community-wide outbreak of infection with a 229E-like coronavirus in Tecumseh, Michigan. Journal of Infectious Diseases 112:272–279
    [Google Scholar]
  6. Dejong J. C. 1970; Decay mechanism of polio and EMC viruses in aerosols. In Proceedings of The Third International Symposium on Aerobiology pp 210 Edited by Silver I. H. New York: Academic Press;
    [Google Scholar]
  7. Dejong J., Trouwborst G. T., Winkler K. C. 1973; The mechanism of virus decay in aerosols. In Airborne Transmission and Airborne Infection pp 124–130 Edited by Hers J. F. P., Winkler K. C. New York: John Wiley & Sons;
    [Google Scholar]
  8. Harper G. J. 1961; Airborne micro-organisms: survival tests with four viruses. Journal of Hygiene 59:479–486
    [Google Scholar]
  9. Hemmes J. H., Winkler K. C., Kool S. M. 1960; Virus survival as a seasonal factor in influenza and poliomyelitis. Nature, London 188:430–431
    [Google Scholar]
  10. Ijaz M. K., Sattar S. A., Johnson-lussenburg C. M., Springthorpe V. S. 1984; Airborne survival of rotaviruses and coronaviruses: implications for disease transmission. In Virus Diseases of Veterinary Importance in South-East Asia and the Western Pacific Edited by J A. Della-Porta. Toronto: Academic Press; (in press)
    [Google Scholar]
  11. Ijaz M. K., Sattar S. A., Johnson-lussenburg C. M., Springthorpe V. S. 1985a; Comparison of the airborne survival of calf rotavirus and poliovirus type 1 (Sabin) aerosolized as a mixture. Applied and Environmental Microbiology 49:289–293
    [Google Scholar]
  12. Ijaz M. K., Sattar S. A., Johnson-lussenburg C. M., Springthorpe V. S. 1985b; Effect of relative humidity and suspending medium on the airborne survival of human rotavirus. Canadian Journal of Microbiology31 (in press)
    [Google Scholar]
  13. Kennedy D. A., Johnson-lussenburg C. M. 1975/76; Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology 6:197–206
    [Google Scholar]
  14. Lawless J. F. 1982 In Statistical Models and Methods for Lifetime Data New York: John wiley & Sons;
    [Google Scholar]
  15. Mcintosh K., Kapikian A. Z., Turner H. C., Hartley J. W., Parrott R. H., Chanock R. M. 1970; Seroepidemiologic studies of coronavirus infection in adults and children. American Journal of Epidemiology 91:585–592
    [Google Scholar]
  16. Macnaughton M. R. 1982; Occurrence and frequency of coronavirus infections in humans as determined by enzyme-linked immunosorbent assay. Infection and Immunity 38:419–423
    [Google Scholar]
  17. Macnaughton M. R., Davies H. A. 1981; Human enteric coronaviruses. Archives of Virology 70:301–313
    [Google Scholar]
  18. Riski H., Hovi T. 1980; Coronavirus infections of man associated with diseases other than the common cold. Journal of Medical Virology 6:259–265
    [Google Scholar]
  19. Robb J. A., Bond C. W. 1979; Coronaviridae. In Comprehensive Virology vol 14: pp 193–247 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  20. Sattar S. A., Ijaz M. K., Johnson-lussenburg C. M., Springthorpe V. S. 1984; Effect of relative humidity on the airborne survival of rotavirus SA-U. Applied and Environmental Microbiology 47:879–881
    [Google Scholar]
  21. Wege H., Siddell S. G., Ter meulen V. 1982; The biology and pathogenesis of coronaviruses. Current Topics in Microbiology and Immunology 99:165–200
    [Google Scholar]
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