translation of virion RNA from carnation mottle virus (CarMV) in a messenger RNA-dependent rabbit reticulocyte lysate (MDL) resulted in the synthesis of four virus-specific polypeptides of apparent 100000 (P100), 77000 (P77), 38000 (P38) and 30000 (P30). Partial peptide mapping experiments and translation in the presence of partially purified calf liver amber suppressor tRNA demonstrated that P30, P77 and P100 are a series of overlapping polypeptides generated by a double readthrough mechanism. In addition we report the infection of protoplasts with CarMV. The viral coat protein was detected in virus-infected protoplasts. Only one other infection-specific protein with an apparent of 100000 corresponded in size to any of the other translation products.


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