1887

Abstract

SUMMARY

translation of virion RNA from carnation mottle virus (CarMV) in a messenger RNA-dependent rabbit reticulocyte lysate (MDL) resulted in the synthesis of four virus-specific polypeptides of apparent 100000 (P100), 77000 (P77), 38000 (P38) and 30000 (P30). Partial peptide mapping experiments and translation in the presence of partially purified calf liver amber suppressor tRNA demonstrated that P30, P77 and P100 are a series of overlapping polypeptides generated by a double readthrough mechanism. In addition we report the infection of protoplasts with CarMV. The viral coat protein was detected in virus-infected protoplasts. Only one other infection-specific protein with an apparent of 100000 corresponded in size to any of the other translation products.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-12-2597
1985-12-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/12/JV0660122597.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-12-2597&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Carrington J. C., Morris T. J. 1984; Complementary DNA cloning and analysis of carnation mottle virus RNA. Virology 139:22–31
    [Google Scholar]
  3. Carrington J. C., Morris T. J. 1985; Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs. Virology 144:1–10
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. De Varennes A., Russo M., Maule A. J. 1984; Infection of protoplasts from Chenopodium quinoa with cowpea mosaic and cymbidium ringspot viruses. Journal of General Virology 65:1851–1855
    [Google Scholar]
  6. Harbison S. A., Wilson T. M. A., Davies J. W. 1984; An encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus. Bioscience Reports 4:949–956
    [Google Scholar]
  7. Kummert J., Kettmann R. 1978; Reverse transcription of turnip yellow mosaic virus RNA primed with calf thymus DNA hydrolysate: characterization of the purified cDNA product. Nucleic Acids Research 5:4428–4430
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual pp 466–467 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Maule A. J., Boulton M. I., Wood K. R. 1980; An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathologisch Zeitschrift 97:118–126
    [Google Scholar]
  11. Morris-Krsinich B. A. M., Forster R. L. S. 1983; Lucerne transient streak virus RNA and its translation in rabbit reticulocyte lysate and wheat germ extract. Virology 128:176–185
    [Google Scholar]
  12. Pelham H. R. B. 1978; Leaky UAG termination codon in tobacco mosaic virus RNA. Nature, London 272:469–471
    [Google Scholar]
  13. Pelham H. R. B. 1979; Translation of tobacco rattle virus RNAs in vitro: four proteins from three RNAs. Virology 97:256–265
    [Google Scholar]
  14. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  15. Rottier P. J. M., Rezelman G., Van kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  16. Salomon R., Bar-Joseph M., Soreq H., Gozes I., Littauer U. Z. 1978; Translation in vitro of carnation mottle virus RNA. Regulatory function of the 3′ region. Virology 90:288–298
    [Google Scholar]
  17. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  18. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U. S. A 77:5201–5205
    [Google Scholar]
  19. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining the viability of cultured plant cells. Slain Technology 47:189–194
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-12-2597
Loading
/content/journal/jgv/10.1099/0022-1317-66-12-2597
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error