1887

Abstract

SUMMARY

A nuclear polyhedrosis virus (NPV) strain from (SeMNPV-25 baculovirus) is a restriction endonuclease DNA map variant similar to NPV (AcMNPV baculovirus). Fourteen restriction endonuclease variants were identified and isolated from a SeMNPV baculovirus stock with 12 of the variants found at low frequency (< 3%). The DNA from each variant was compared to the prototype SeMNPV-25 for insertions, deletions and new restriction sites. Regions of variation were defined on the prototype SeMNPV-25 genome, and the nature of the variation within these regions was determined. These data are discussed and compared with the existing data on other variants of AcMNPV. A comparison of the physical maps revealed that all the SeMNPV variants were different from those reported for AcMNPV. Although the SeMNPV variants were distinctive, they were clearly genomic AcMNPV variants. The regions of the baculovirus genomic variation were identified, and three separate mechanisms are suggested for their generation. Five regions (hr1 to hr5) were associated with intragenic homologous viral sequences, five regions (vI to vIII) may be associated with the insertion of DNA sequences of cellular origin, and two regions (pI and pII) were associated with mutations resulting in the addition or loss of a I site. Physical maps were generated for SeMNPV variant regions vI, hr2, vII and vIII.

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1985-11-01
2024-04-26
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References

  1. Brown S. E., Maruniak J. E., Knudson D. L. 1984; Physical map of SeMNPV baculovirus DNA: an AcMNPV genomic variant. Virology 136:235–240
    [Google Scholar]
  2. Burand J. P., Summers M. D. 1982; Alteration of Autographa californica nuclear polyhedrosis virus DNA upon serial passage in cell culture. Virology 119:223–229
    [Google Scholar]
  3. Cochran M. A., Faulkner P. 1983; Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. Journal of Virology 45:961–970
    [Google Scholar]
  4. Cochran M. A., Carstens E. B., Eaton B. T., Faulkner P. 1982; Molecular cloning and physical mapping of restriction endonuclease fragments of Autographa californica nuclear polyhedrosis virus DNA. Journal of Virology 41:940–946
    [Google Scholar]
  5. Fraser M. J., Hink W. F. 1982; The isolation and characterization of the MP and FP plaque variants of Galieria mellonella nuclear polyhedrosis virus. Virology 117:366–378
    [Google Scholar]
  6. Fraser M. J., Smith G. E., Summers M. D. 1983; Acquisition of host cell DNA sequences by baculoviruses: relationship between host DNA insertions and FP mutants of Autographa californica and Galleria mellonella nuclear polyhedrosis viruses. Journal of Virology 47:287–300
    [Google Scholar]
  7. Harrap K. A., Payne C. C., Robertson J. S. 1977; The properties of three baculoviruses from closely related hosts. Virology 79:14–31
    [Google Scholar]
  8. Hink W. F., Strauss E. 1976; Replication and passage of alfalfa looper nuclear polyhedrosis virus plaque variants in cloned cell cultures and larval stages of four host species. Journal of Invertebrate Pathology 27:4955
    [Google Scholar]
  9. Hink W. F., Vail P. V. 1973; A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus on a cabbage looper (TN-368) cell line. Journal of Invertebrate Pathology 22:168–174
    [Google Scholar]
  10. Holford T. R., Brown S. E., Knudson D. L. 1985; Estimation of DNA fragment size and generation of DNA restriction endonuclease maps using linear models. Journal of Virological Methods 10:117–126
    [Google Scholar]
  11. Knell J. D., Summers M. D. 1981; Investigation of genetic heterogeneity in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified variants. Virology 112:190–197
    [Google Scholar]
  12. Knudson D. L. 1979; Plaque assay of baculoviruses employing an agarose-nutrient overlay. Intervirology 11:40–46
    [Google Scholar]
  13. Knudson D. L., Buckley S. M. 1977; Invertebrate cell culture methods for the study of invertebrate-associated animal viruses. In Methods in Virology vol 6: pp 323–391 Edited by Maramorosch K., Koprowski H. New York: Academic Press;
    [Google Scholar]
  14. Knudson D. L., Harrap K. A. 1976; Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda’. microscopy study of the sequence of events of the virus infection. Journal of Virology 17:254–268
    [Google Scholar]
  15. Knudson D. L., Tinsley T. W. 1974; Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda’. purification, assay of infectivity, and growth characteristics of the virus. Journal of Virology 14:934–944
    [Google Scholar]
  16. Lee H. H., Miller L. K. 1978; Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. Journal of Virology 27:754–767
    [Google Scholar]
  17. Lubbert H., Kruczek L., Tjia S., Doerfler W. 1981; The cloned £coRI fragments of Autographa californica nuclear polyhedrosis virus DNA. Gene 16:343–345
    [Google Scholar]
  18. Mcdonnell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  19. Maruniak J. E., Brown S. E., Knudson D. L. 1984; Physical maps of SfMNPV baculovirus DNA and its genomic variants. Virology 136:221–234
    [Google Scholar]
  20. Miller D. W., Miller L. K. 1982; A virus mutant with an insertion of a copia- like transposable element. Nature, London 299:562–564
    [Google Scholar]
  21. Miller L. K. 1984; Exploring the gene organization of baculoviruses. In Methods in Virology vol 7: pp 227–258 Edited by Maramorosch K., Koprowski H. New York: Academic Press;
    [Google Scholar]
  22. Miller L. K., Dawes K. P. 1978; Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV. Applied and Environmental Microbiology- 35:1206–1210
    [Google Scholar]
  23. Miller L. K., Dawes K. P. 1979; Physical map of the DNA genome of Autographa californica nuclear polyhedrosis virus. Journal of Virology 29:1044–1055
    [Google Scholar]
  24. Miller L. K., Franzblau S. G., Homan H. W., Kish L. P. 1980; A new variant of Autographa californica nuclear polyhedrosis virus. Journal of Invertebrate Pathology 36:159–165
    [Google Scholar]
  25. Potter K. N., Faulkner P., MacKinnon E. A. 1976; Strain selection during serial passage of Trichoplusia ni nuclear polyhedrosis virus. Journal of Virology 18:1040–1050
    [Google Scholar]
  26. Potter K. N., Jaques R. P., Faulkner P. 1978; Modification of Trichoplusia ni nuclear polyhedrosis virus passaged in vivo. Intervirology 9:76–85
    [Google Scholar]
  27. Ramoska W. A., Hink W. F. 1974; Electron microscope examination of two plaque variants from a nuclear polyhedrosis virus of the alfalfa looper, Autographa californica. Journal of Invertebrate Pathology 23:197–201
    [Google Scholar]
  28. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 89:517–527
    [Google Scholar]
  29. Smith G. E., Summers M. D. 1979; Restriction maps of five Autographa californica MNPV variants, Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with endonucleases Sma I, Kpn I, Bam HI, Sac I, Xhol, and Uco RI. Journal of Virology 30:828–838
    [Google Scholar]
  30. Tweeten K. A., Bulla L. A. jr, Consigli R. A. 1980; Restriction enzyme analysis of the genomes of Plodia interpunctella and Pier is rapae granulosis viruses. Virology 104:514–519
    [Google Scholar]
  31. Wood H. A. 1980; Isolation and replication of an occlusion body-deficient mutant of the Autographa californica nuclear polyhedrosis virus. Virology 105:338–344
    [Google Scholar]
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