1887

Abstract

SUMMARY

The multiplication of the M9, A7 and L10 strains of Semliki Forest virus (SFV), both in weanling mice and primary mouse brain cell cultures, was compared. Following both intraperitoneal (i.p.) and intracerebral (i.c.) injection, the virulent L10 strain multiplied to higher titre in the mouse central nervous system (CNS) than did the less virulent M9 and A7 strains, whereas M9 multiplied to higher titre than A7. By the i.c. route, all three virus strains multiplied to higher titre than following i.p. injection. Multiplication of A7 and M9 in oligodendrocytes, but not neurons, was detected following i.c. injection. All three virus strains showed a tropism for cultured mouse glial cells rather than neurons. The L10 strain multiplied better in neurons than did A7 or M9. It is concluded that the mechanism of acute demyelination induced by the M9 and A7 strains is similar. Based on this and previous studies, it is proposed that infection of glial cells triggers immune-mediated demyelination. The virulence of the L10 strain is due to its ability to exceed a lethal threshold of damage to neurons before immune intervention can occur.

Keyword(s): demyelination , pathogenicity and SFV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-11-2365
1985-11-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/11/JV0660112365.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-11-2365&mimeType=html&fmt=ahah

References

  1. Atkins G. J. 1983; The avirulent A7 strain of Semliki Forest virus has reduced cytopathogenicity for neuroblastoma cells compared to the virulent L10 strain. Journal of General Virology 64:1401–1404
    [Google Scholar]
  2. Atkins G. J., Sheahan B. J. 1982; Semliki Forest virus neurovirulence mutants have altered cytopathogenicity for central nervous system cells. Infection and Immunity 36:333–341
    [Google Scholar]
  3. Atkins G. J., Sheahan B. J., Dimmock N. J. 1985; Semliki Forest virus infection of mice: a model for genetic and molecular analysis of viral pathogenicity. Journal of General Virology 66:395–408
    [Google Scholar]
  4. Barrett P. N., Sheahan B. J., Atkins G. J. 1980; Isolation and preliminary characterization of Semliki Forest virus mutants with altered virulence. Journal of General Virology 49:141–147
    [Google Scholar]
  5. Berger M. L. 1980; Humoral and cell-mediated immune mechanisms in the production of pathology in avirulent Semliki Forest virus encephalitis. Infection and Immunity 30:244–253
    [Google Scholar]
  6. Bradish C. I., Allner K., Maber H. B. 1971; The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs and rabbits. Journal of General Virology 12:141–160
    [Google Scholar]
  7. Bruce C. B., Chapman J., Suckling A. I., Rumsby M. G. 1984; Infection of rat brain primary cell cultures with an avirulent A7 strain of Semliki Forest virus. Journal of the Neurological Sciences 66:77–90
    [Google Scholar]
  8. Chapman J., Rumsby M. G. 1982; A syringe modification for the simple and rapid dissociation of post-natal rat cerebral tissue for preparing primary cultures of mixed glial cells. Neuroscience Letters 34:307–314
    [Google Scholar]
  9. Chew-Lim M. 1979; Brain viral persistence and myelin damage in nude mice. Canadian Journal of Comparative Medicine 43:39–43
    [Google Scholar]
  10. Chew-Lim M., Webb H. E., Jagelman S. 1977; The effect of irradiation on demyelination induced by avirulent Semliki Forest virus. British Journal of Experimental Pathology 58:459–464
    [Google Scholar]
  11. Gates M. C., Sheahan B. J., Atkins G. J. 1984; The pathogenicity of the M9 mutant of Semliki Forest virus in immune-compromised mice. Journal of General Virology 65:73–80
    [Google Scholar]
  12. Jagelman S., Suckling A. J., Webb H. E., Bowen E. T. W. 1978; The pathogenesis of avirulent Semliki Forest virus infections in athymic nude mice. Journal of General Virology 41:599–607
    [Google Scholar]
  13. Pathak S., Webb H. E. 1978; An electron microscopic study of avirulent and virulent Semliki Forest virus in the brains of different ages of mice. Journal of the Neurological Sciences 39:199–211
    [Google Scholar]
  14. Pusztai R., Gould E. A., Smith H. 1971; Infection pattern in mice of an avirulent and virulent strain of Semliki Forest virus. British Journal of Experimental Pathology 52:669–677
    [Google Scholar]
  15. Sheahan B. J., Barrett P. N., Atkins G. J. 1981; Demyelination in mice from infection with a mutant of Semliki Forest virus. Acta neuropathologica 53:129–136
    [Google Scholar]
  16. Sheahan B. I., Gates M. C., Caffrey J. F., Atkins G. J. 1983; Oligodendrocyte infection and demyelination produced in mice by the M9 mutant of Semliki Forest virus. Acta neuropathologica 60:257–265
    [Google Scholar]
  17. Wilkin G. P. W., Wilson J. E., Balazs R., Cohen J., Dutton L. R. 1976; Preparation of cell bodies from the developing cerebellum: structural and molecular integrity of the isolated cells. Brain Research 115:181–199
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-11-2365
Loading
/content/journal/jgv/10.1099/0022-1317-66-11-2365
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error