1887

Abstract

SUMMARY

Two of the genes identified from the previously published DNA sequence of the U component of the varicella-zoster virus (VZV) genome were predicted to encode membrane proteins with polypeptide molecular weights of 39 000 (39K) and 70K. A rabbit antiserum directed against a unique peptide containing the seven amino acid residues at the carboxy terminus of the 39K gene product specifically precipitated glycoproteins with apparent molecular weights of 55K and 45K from VZV-infected cells labelled with [H]mannose. The complete inhibition of precipitation of gp55 by free peptide and the partial inhibition of precipitation of gp45 support the conclusion that the 39K gene encodes gp55 and perhaps gp45. The number of VZV genes currently thought to encode glycoproteins is discussed in view of this finding.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-10-2237
1985-10-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/10/JV0660102237.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-10-2237&mimeType=html&fmt=ahah

References

  1. Asano Y., Takahashi M. 1979; Studies on the polypeptides of varicella-zoster (V-Z) virus. I. Detection of varicella-zoster virus polypeptides in infected cells. Biken Journal 22:81–89
    [Google Scholar]
  2. Davison A. J. 1983; DNA sequence of the U5 component of the varicella-zoster virus genome. EMBO Journal 2:2203–2209
    [Google Scholar]
  3. Dumas A. M., Geelen J. L. M. C., Weststrate M. W., Wertheim P., Van der Noordaa J. 1981; XbaI, PstI and BgIII restriction enzyme maps of the two orientations of the varicella-zoster virus genome. Journal of Virology 39:390–400
    [Google Scholar]
  4. Ellis R. W., Keller P. M., Lowe R. S., Zivin R. A. 1985; Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC. Journal of Virology S3:81–88
    [Google Scholar]
  5. Forghani B., Dupuis K. W., Schmidt N. J. 1984; Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies. Journal of Virology 52:55–62
    [Google Scholar]
  6. Grose C. 1980; The synthesis of glycoproteins in human melanoma cells infected with varicella-zoster virus. Virology 101:1–9
    [Google Scholar]
  7. Grose C., Friedrichs W. E. 1982; Immunoprecipitable polypeptides specified by varicella-zoster virus. Virology 118:86–95
    [Google Scholar]
  8. Grose C., Edwards D. P., Friedrichs W. E., Weigle K. A., Mcguire W. L. 1983; Monoclonal antibodies against three major glycoproteins of varicella-zoster virus. Infection and Immunity 40:381–388
    [Google Scholar]
  9. Grose C., Edwards D. P., Weigle K. A., Friedrichs W. E., Mcguire W. L. 1984; Varicella-zoster virus-specific gp 140: a highly immunogenic and disulphide-linked structural glycoprotein. Virology 132:138–146
    [Google Scholar]
  10. Keller P. M., Neff B. J., Ellis R. W. 1984; Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes. Journal of Virology 52:293–297
    [Google Scholar]
  11. Mcgeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  12. Okuno T., Yamanishi K., Shiraki K., Takahashi M. 1983; Synthesis and processing of glycoproteins of varicella-zoster virus (VZV) as studied with monoclonal antibodies to VZV antigens. Virology 129:357–368
    [Google Scholar]
  13. Preston V. G., Palfreyman J. W., Dutia B. M. 1984; Identification of a herpes simplex virus type 1 polypeptide which is a component of the virus-induced ribonucleotide reductase. Journal of General Virology 65:14571466
    [Google Scholar]
  14. Shemer Y., Leventon-Kriss S., Sarov I. 1980; Isolation and polypeptide characterization of varicella-zoster virus. Virology 106:133–140
    [Google Scholar]
  15. Shiraki K., Okuno T., Yamanishi K., Takahashi M. 1982; Polypeptides of varicella-zoster virus (VZV) and immunological relationship of VZV and herpes simplex virus (HSV). Journal of General Virology 61:255–269
    [Google Scholar]
  16. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP4. Infection and Immunity 34:684–692
    [Google Scholar]
  17. Vafai A., Wroblewska Z., Wellish M., Green M., Gilden D. 1984; Analysis of three late varicella-zoster virus proteins, a 125, 000-molecular-weight protein and gpl and gp3. Journal of Virology 52:953–959
    [Google Scholar]
  18. Zweerink H., Neff B. J. 1981; Immune response after exposure to varicella zoster virus: characterization of virus-specific antibodies and their corresponding antigens. Infection and Immunity 31:436–444
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-66-10-2237
Loading
/content/journal/jgv/10.1099/0022-1317-66-10-2237
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error