1887

Abstract

SUMMARY

Mitogen treatment of murine (BALB/c) B-cells induces two different endogenous retroviruses involving two unlinked, presumably proviral, loci Bxv-1 and Bdv-1. To determine the usefulness of these loci as genetic markers for B-cell differentiation their expression was studied under conditions that interfered with B-cell proliferation and differentiation into IgM-secreting plaque-forming cells (p.f.c.). Maximum production of both viruses followed peak DNA synthesis by an interval of about 18 h. Treatments that blocked DNA synthesis or killed proliferating cells inhibited virus production. Addition of BUdR to mitogen-stimulated cultures selectively induced Bxv-1 while inhibiting the generation of p.f.c. Both effects require BUdR incorporation into the DNA of proliferating cells. 5-Azacytidine induced Bxv-1-dependent virus production without inhibiting terminal B-cell differentiation. Pretreatment of mitogen-stimulated B-cells with anti-mouse IgM serum decreased both virus production and generation of p.f.c., but had little effect on DNA synthesis. Experiments using a mitogenic F(ab′) preparation of anti-IgM in the presence and absence of lymphokines also suggested that the generation of p.f.c. and Bxv-1-dependent virus production are linked phenomena. The data imply that Bxv-1- and Bdv-1-dependent virus production require DNA synthesis and cell proliferation and, at least for Bxv-1, B-cell differentiation. It is proposed that the induction of these loci reflects the involvement of neighbouring DNA sequences in B-cell proliferation or differentiation.

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1985-01-01
2022-09-25
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References

  1. Alberto B. P., Callahan L. F., Pincus T. 1982; Evidence that retrovirus expression in mouse spleen cells results from B-cell differentiation. Journal of Immunology 129:2768–2772
    [Google Scholar]
  2. Andersson J., Bullock W. W., Melchers F. 1974; Inhibition of mitogenic stimulation of mouse lymphocytes by anti-mouse immunoglobulin antibodies. I. Mode of action. European Journal of Immunology 4:715–722
    [Google Scholar]
  3. Andersson J., Coutinho A., Melchers F. 1978; Stimulation of murine B-cells to IgG synthesis and secretion by the mitogens lipopolysaccharide and lipoprotein and its inhibition by anti-immunoglobulin. European Journal of Immunology 8:336–343
    [Google Scholar]
  4. Andersson J., Schreier M. H., Melchers F. 1980; T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level. Proceedings of the National Academy of Sciences, U.S.A 77:1612–1616
    [Google Scholar]
  5. Besmer P., Smotkin D., Haseltine W., Fan H., Wilkson A. T., Paskind M., Weinberg R., Baltimore D. 1975; Mechanism of induction of RNA tumor viruses by halogenated pyrimidines. Cold Spring Harbor Symposia on Quantitative Biology 39:1103–1107
    [Google Scholar]
  6. Blatt C., Mileham K., Haas M., Nesbitt M. N., Harper M. E., Simon M. I. 1983; Chromosomal mapping of the mink cell-focus inducing and xenotropic env gene family in the mouse. Proceedings of the National Academy of Sciences, U.S.A 80:6298–6302
    [Google Scholar]
  7. Burch J. B. E., Weintraub H. 1983; Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene. Cell 33:65–76
    [Google Scholar]
  8. Coffin J. 1982; Endogenous viruses. In Molecular Biology of RNA Tumor Viruses pp 1109–1203 Edited by Weiss R., Teich N., Varmus H., Coffin J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Conklin K. F., Coffin J. M., Robinson H. L., Groudine M., Eisenman R. 1982; Role of methylation in the induced and spontaneous expression of the avian endogenous virus ev-1: DNA structure and gene products. Molecular and Cellular Biology 2:638–652
    [Google Scholar]
  10. Cooper G. M., Silverman L. 1978; Linkage of the endogenous avian leukosis virus genome of virus-producing chicken cells to inhibitory cellular DNA sequences. Cell 15:573–577
    [Google Scholar]
  11. Copeland N. G., Cooper G. M. 1979; Transfection of exogenous and endogenous murine retrovirus DNAs. Cell 16:347–356
    [Google Scholar]
  12. Delamarter J., Monckton R. P., Moroni C. 1981; Transcriptional control of endogenous virus genes in murine lymphocytes. Journal of General Virology 52:371–375
    [Google Scholar]
  13. Elder J. H., Jensen F. C., Bryant M. L., Lerner R. A. 1977; Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family. Nature, London 267:23–28
    [Google Scholar]
  14. Greenberger J. S., Phillips S. M., Stephenson J. R., Aaronson S. A. 1975; Induction of mouse type-C virus by lipopolysaccharide. Journal of Immunology 115:317–320
    [Google Scholar]
  15. Gronowicz E., Coutinho A., Melchers F. 1976; A plaque assay for all cells secreting Ig of a given type or class. European Journal of Immunology 6:588–590
    [Google Scholar]
  16. Groudine M., Eisenman R., Weintraub H. 1981; Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA méthylation. Nature, London 292:311–317
    [Google Scholar]
  17. Jaenisch R., Jähner D., Nobis P., Simon I., Löhler J., Harber K., Grotkopp D. 1981; Chromosomal position and activation of retroviral genomes inserted into the germ line of mice. Cell 24:519–529
    [Google Scholar]
  18. Janossy G., Snajdr J, Simak-Elus M. 1976; Patterns of B lymphocyte gene expression elicited by lipopolysaccharide mitogen. Immunology 30:799–810
    [Google Scholar]
  19. Jongstra J. 1981; The expression of endogenous C-type viruses in mouse lymphocyte cultures. Ph.D. thesis University of Basel;
    [Google Scholar]
  20. Kozak C. A., Rowe W. P. 1980; Genetic mapping of xenotropic murine leukemia virus-inducing loci in five mouse strains. Journal of Experimental Medicine 152:219–228
    [Google Scholar]
  21. Larsson E.-L., Iscove N. N., Coutinho A. 1980; Two distinct factors are required for the induction of T-cell growth. Nature, London 283:664–666
    [Google Scholar]
  22. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. 1971; Murine leukemia virus: high frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science 174:155–156
    [Google Scholar]
  23. Melchers F., Andersson J. 1974; The kinetics of proliferation and maturation of mitogen-activated bone marrow-derived lymphocytes. European Journal of Immunology 4:181–188
    [Google Scholar]
  24. Meruelo D., Rossomando A., Offer M., Buxbaum J., Pellicer A. 1983; Association of endogenous viral loci with genes encoding murine histocompatibility and lymphocyte differentiation antigens. Proceedings of the National Academy of Sciences, U.S.A 80:5032–5036
    [Google Scholar]
  25. Monckton R. P., Moroni C. 1980; Foetal calf serum acts as inducer of endogenous C-type virus in mouse lymphoid cells. Journal of General Virology 47:59–66
    [Google Scholar]
  26. Moroni C., Schumann G. 1975; Lipopolysaccharide induces C-type virus in short term cultures of BALB/c spleen cells. Nature, London 254:60–61
    [Google Scholar]
  27. Moroni C., Schumann G. 1976; Mitogen induction of murine C-type viruses. II. Effect of B-lymphocyte mitogens. Virology 73:17–22
    [Google Scholar]
  28. Moroni C., Schumann G., Robert-Guroff M., Suter E. R., Martin D. 1975; Induction of endogenous murine C-type virus in spleen cell cultures treated with mitogens and 5-bromo-2′-deoxyuridine. Proceedings of the National Academy of Sciences, U.S.A 72:535–538
    [Google Scholar]
  29. Moroni C., Matter A., Stoye J. P., Monckton R. P., Delamarter J. F., Schumann G. 1980a; Concanavalin A promotes bromodeoxyuridine induction of endogenous C-type virus in B cells. Cellular Immunology 54:107–114
    [Google Scholar]
  30. Moroni C., Stoye J. P., Delamarter J. F., Erb P., Jay F. A., Jongsttra J., Martin D., Schumann G. 1980b; Normal B-cell activation involves endogenous retroviral antigen expression: implications for leukemo-genesis. Cold Spring Harbor Symposia on Quantitative Biology 44:1205–1210
    [Google Scholar]
  31. Niwa O., Sugahara T. 1981; 5-azacytidine induction of mouse endogenous type C virus and suppression of DNA méthylation. Proceedings of the National Academy of Sciences, U.S.A 78:6290–6294
    [Google Scholar]
  32. Palacios R. 1982; Mechanism of T cell activation: role and functional relationship of HLA-DR antigens and interleukins. Immunological Reviews 63:73–110
    [Google Scholar]
  33. Parker D. C., Wadsworth D. C., Schneider G. B. 1980; Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion. Journal of Experimental Medicine 152:138–150
    [Google Scholar]
  34. Peebles P. T. 1975; An in vitro focus-induction assay for xenotropic murine leukemia virus, feline leukemia virus C and the feline-primate viruses RD-114/CCC/M7. Virology 67:288–291
    [Google Scholar]
  35. Razin A., Riggs A. D. 1980; DNA méthylation and gene function. Science 210:604–610
    [Google Scholar]
  36. Scher L., Ahmed A., Strong D. M., Steinberg A. D., Dart W. E. 1975; X-Linked B-lymphocyte immune defect in CBA/N mice. Journal of Experimental Medicine 141:788–803
    [Google Scholar]
  37. Schumann G., Moroni C. 1976; Mitogen induction of murine C-type viruses. I. Analysis of lymphoid cell subpopulations. Journal of Immunology 116:1145–1150
    [Google Scholar]
  38. Stoye J. P., Moroni C. 1981; Effect of bromodeoxyuridine on endogenous retrovirus production in differentiating murine lymphocytes. In Modern Trends in Human Leukemia IV pp 365–367 Edited by Neth R., Gallo R., Graf T., Mannweiler K., Winkler K. Berlin: Springer-Verlag;
    [Google Scholar]
  39. Stoye J. P., Moroni C. 1983; Endogenous retrovirus expression in stimulated murine lymphocytes: identification of a new locus controlling mitogen induction of a defective virus. Journal of Experimental Medicine 157:1660–1674
    [Google Scholar]
  40. Stoye J. P., Moroni C. 1984; Phenotypic mixing of retroviruses in mitogen-stimulated lymphocytes: analysis of xenotropic and defective endogenous mouse viruses. Journal of General Virology 65:317–326
    [Google Scholar]
  41. Strand M., Lilly F., August J. T. 1974; Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains. Proceedings of the National Academy of Sciences, U.S.A 71:3682–3686
    [Google Scholar]
  42. Teich N., Lowy D. R., Hartley J. W., Rowe W. P. 1973; Studies of mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine. Virology 51:163–173
    [Google Scholar]
  43. Thomson J. A., Laipis P. J., Stein G. S., Stein J. L., Lander M. R., Chattopadhyay S. 1980; Regulation of endogenous type C viruses: evidence for transcriptional control of AKR viral expression. Virology 101:529–533
    [Google Scholar]
  44. Wecker E., Schimpl A., Hunig T. 1977; Expression of MuLV gp71-like antigen in normal mouse spleen cells induced by antigenic stimulation. Nature, London 269:598–600
    [Google Scholar]
  45. Wilks A. F., Cozens P. J., Mattaj I. W., Jost J.-P. 1982; Estrogen induces a déméthylation at the 5′ end region of the chicken vitellogenin gene. Proceedings of the National Academy of Sciences, U.S.A 79:4252–4255
    [Google Scholar]
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