1887

Abstract

SUMMARY

The characteristics of a reovirus (CRV) recently isolated from channel catfish () were examined following purification of virions from infected cell cultures. Virions had double capsids, the inner and outer with diameters of 55 nm and 75 nm respectively. Complete virions had a density of 1.36 g/ml in CsCl gradients and contained seven polypeptides of 132000, 130000, 110000, 68000, 56000, 43000 and 32000 mol. wt. The nucleic acid labelled with [5-H]uridine had a density of 1.56 g/ml in CsSO gradients, which suggested that it is double-stranded RNA. The genome was composed of 11 segments that ranged in mol. wt. from approximately 0.4 × 10 to 2.5 × 10. Serum cross-neutralization comparisons of CRV to reoviruses isolated from chum salmon () and golden shiners () indicated that each was distinct although some crossreactions were observed. These viruses seem to represent three serotypes of a new genus within the family Reoviridae.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-9-1527
1984-09-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/9/JV0650091527.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-9-1527&mimeType=html&fmt=ahah

References

  1. Amend D. F., Mcdowell T., Hedrick R. P. 1984; Characteristics of a previously unidentified virus from channel catfish (ictalurus punctatus). Canadian Journal of Fisheries and Aquatic Sciences (in press)
    [Google Scholar]
  2. Archetti I., Horsfall F. L. 1950; Persistent antigenic variation of influenza A viruses after incomplete neutralization in vivo with heterologous immune serum. Journal of Experimental Medicine 92:441–462
    [Google Scholar]
  3. Bowser P. R., Plumb J. A. 1980; Fish cell lines: establishment of a line from ovaries of channel catfish. in Vitro 16:365–368
    [Google Scholar]
  4. Dobos P., Hill B. J., Hallett R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented genomes. Journal of Virology 32:593–605
    [Google Scholar]
  5. Fields B. N. 1982; Molecular basis of reovirus virulence. Archives of Virology 71:95–107
    [Google Scholar]
  6. Hedrick R. P., Okamoto N., Sano T., Fryer J. L. 1983; Biochemical characterization of eel virus European. Journal of General Virology 64:1421–1426
    [Google Scholar]
  7. Hill B. J. 1982; Infectious pancreatic necrosis virus and its virulence. In Microbial Diseases of Fish pp 91–114 Edited by Roberts R. J. London: Academic Press;
    [Google Scholar]
  8. Iglewski W. J., Franklin R. M. 1967; Purification and properties of reovirus ribonucleic acid. Journal of Virology 1:302–307
    [Google Scholar]
  9. Joklik W. K. 1981; Structure and function of the reovirus genome. Microbiological Reviews 45:483–501
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Ludlum D. B., Warner R. C. 1965; Equilibrium centrifugation in cesium sulfate solutions. Journal of Biological Chemistry 240:2961–2965
    [Google Scholar]
  12. Macdonald R. D., Yamamoto T. 1977; The structure of infectious pancreatic necrosis virus RNA. Journal of General Virology 34:235–247
    [Google Scholar]
  13. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:143–144
    [Google Scholar]
  14. Meyers T. R. 1979; A reo-like virus isolated from juvenile American oysters (Crassostrea virginica). Journal of General Virology 43:203–212
    [Google Scholar]
  15. Meyers T. R. 1980; Experimental pathogenicity of reovirus 13p2 for juvenile American oysters Crassostrea virginica (Gmelin) and bluegill fingerlings Lepomis macrochirus Rafinesque. Journal of Fish Diseases 3:187201
    [Google Scholar]
  16. Nims L., Fryer J. L., Pilcher K. S. 1970; Studies of replication of four selected viruses in two cell lines derived from salmonid fish. Proceedings of the Society for Experimental Biology and Medicine 135:6–12
    [Google Scholar]
  17. Okamoto N., Sano T., Hedrick R. P., Fryer J. L. 1983; Antigenic relationships of selected strains of infectious pancreatic necrosis virus (IPNV) and eel virus European (EVE). Journal of Fish Diseases 6:19–25
    [Google Scholar]
  18. Palmer E. L., Martin M. L. 1982; Reoviridae. In Atlas of Mammalian Viruses pp 22–33 Florida: CRC Press;
    [Google Scholar]
  19. Plumb J. A., Bowser P. R., Grizzle J. M., Mitchell A. J. 1979; Fish viruses. A double-stranded RNA icosahedral virus from a North American cyprinid. Journal of the Fisheries Research Board of Canada 36:1390–1394
    [Google Scholar]
  20. Price C. A. 1982; Sedimentation-diffusion equilibrium in a density gradient. In Centrifugation in Density Gradients p 258 New York: Academic Press;
    [Google Scholar]
  21. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent end points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  22. Schwedler J. A., Plumb T. E. 1980; Fish viruses: serological comparison of golden shiner and infectious pancreatic necrosis viruses. Journal of Wildlife Diseases 16:597–599
    [Google Scholar]
  23. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top component and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  24. Winton J. R. 1981; Isolation and characterization of a new reovirus of chum salmon (Oncorhynchus keta). Ph.D. thesis Oregon State University;
    [Google Scholar]
  25. Winton J. R., Lannan C. N., Fryer J. L., Kimura T. 1981; Isolation of a new reovirus from chum salmon in Japan. Fish Pathology 15:155–162
    [Google Scholar]
  26. Winton J. R., Lannan C. N., Fryer J. L. 1983; Further characterization of a new reovirus of poikiliothermic vertebrates. In Double-stranded RNA Viruses pp 231–236 Edited by Compans R. W., El D., Bishop L. New York: Elsevier;
    [Google Scholar]
  27. Wolf K., Mann J. A. 1980; Poikiliothermic vertebrate cell lines and viruses: a current listing for fishes. In Vitro 16:168–179
    [Google Scholar]
  28. Wolf K., Snieszko S. F., Dunbar C. E., Pyle E. 1960; Virus nature of infectious pancreatic necrosis in trout. Proceedings of the Society for Experimental Biology and Medicine 104:105–108
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-9-1527
Loading
/content/journal/jgv/10.1099/0022-1317-65-9-1527
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error