1887

Abstract

SUMMARY

Simultaneous infection of MDCK cells with influenza viruses A/WSN and B/Kanagawa resulted in mutual interference with virus protein synthesis and in significant suppression of A/WSN growth. When infection by one virus preceded the other by 1 or 2 h, growth of the superinfecting virus was selectively inhibited at the level of transcription. Interference by the pre-infecting virus was strongly dependent on the expression of the viral genome but not on haemagglutinin activity. When the replication of both virus types was restricted to primary transcription by cycloheximide, the only translation products following removal of the drug were those of the preinfecting virus. This result was not affected by blocking secondary transcription by actinomycin D. These findings suggest that intertypic interference occurs at the level of primary transcription. This concept was supported further by the observation that a mutant of A/WSN (-65) with a defect in primary transcription interfered only with superinfection by B/K anagawa at the permissive temperature.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-8-1385
1984-08-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/8/JV0650081385.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-8-1385&mimeType=html&fmt=ahah

References

  1. Barry R. D., Ives D. R., Cruickshank I. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature, London 194:1139–1140
    [Google Scholar]
  2. Desselberger U., Palese P. 1978; Molecular weight of RNA segments of influenza A and B viruses. Virology 88:394–399
    [Google Scholar]
  3. Gotlieb T., Hirst G. K. 1954; The experimental production of combination forms of virus. III. The formation of doubly antigenic particles from influenza A and B virus and study of the ability of individual particles to yield two separate strains. Journal of Experimental Medicine 99:307–320
    [Google Scholar]
  4. Kaverin N. V., Varich N. L., Sklyanskaya E. I., Amvrosieva T. V., Petrik J., Vovk T. C. 1983; Studies On heterotypic interference between influenza A and B viruses: a differential inhibition of the synthesis of viral proteins and RNAs. Journal of General Virology 64:2139–2146
    [Google Scholar]
  5. Krug R. M., Ueda M., Palese P. 1975; Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis. Journal of Virology 16:790–796
    [Google Scholar]
  6. Maeno K., Kilbourne E. D. 1970; Developmental sequence and intracellular sites of synthesis of three structural antigens of influenza A2 virus. Journal of Virology 5:153–164
    [Google Scholar]
  7. Maeno K., Yoshii S., Mita K., Hamaguchi M., Yoshida T., Iinuma M., Nagai Y., Matsumoto T. 1979; Analysis of the inhibitory effect of canavanine on the replication of influenza RI/5+ virus. I. Inhibition of assembly of RNP. Virology 94:128–137
    [Google Scholar]
  8. Mikheeva A., Ghendon Y. Z. 1982; Intrinsic interference between influenza A and B virus. Archives of Virology 73:287–294
    [Google Scholar]
  9. Pons M. 1973; The inhibition of influenza virus RNA synthesis by actinomycin D and cycloheximide. Virology 51:120–128
    [Google Scholar]
  10. Racaniello V. R., Palese P. 1979; Influenza B Genome: Assignment Of Viral Polypeptides To Rna Segments. Journal of Virology 29:361–373
    [Google Scholar]
  11. Rott R., Orlich M., Scholtissek C. 1981; Intrinsic interference between swine influenza and fowl plague virus. Archives of Virology 69:25–32
    [Google Scholar]
  12. Scholtissek C., Rott R. 1970; Synthesis in vitro of influenza virus plus and minus strand RNA and its preferential inhibition by antibodies. Virology 40:989–996
    [Google Scholar]
  13. Seo H., Vassart G., Brocas H., Refetoff S. 1977; Triiodothreonine stimulates specifically growth hormone mRNA in rat pituitary tumor cells. Proceedings of the National Academy of Sciences, U,. S,. A 74:2054–2058
    [Google Scholar]
  14. Shibata M., Maeno K., Isomura S., Tsurumi T., Aoki H., Suzuki S. 1982; Plaque formation by influenza B virus in a porcine kidney cell line. Microbiology and Immunology 26:441–444
    [Google Scholar]
  15. Sugiura A. 1975 In The Influenza Viruses and Influenza pp 171–213 Edited by Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  16. Sugiura A., Ueda M., Tobita K., Enomoto C. 1975; Further isolation and characterization of temperature-sensitive mutants of influenza virus. Virology 65:363–373
    [Google Scholar]
  17. Tereba A., Mccarthy B. J. 1973; Hybridization of 125I-labelled ribonucleic acid. Biochemistry 12:4675–4679
    [Google Scholar]
  18. Tobita K., Ohori K. 1979; Heterotypic interference between A/Aichi/2/68 and B/Massachusetts/1/71. Acta virologica 23:263–266
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-8-1385
Loading
/content/journal/jgv/10.1099/0022-1317-65-8-1385
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error