1887

Abstract

SUMMARY

Foetal mouse glial cultures were inoculated with murine K papovavirus and subjected to serial subcultivation. Two cell lines were developed. The first of these, KVBCG2A, remained positive for viral infectivity and K virus capsid (V) antigen for over 30 subcultivations. Productive infection was not abolished by serial subcultivation in the presence of antiviral antibody. The second cell line, KVBCG1B, became negative for infectious virus and K virus V antigen, could be cloned from single cells and produced tumours in mice. Sera from tumour-bearing animals produced nuclear fluorescence of KVBCG1B cells and K virus-infected mouse embryo cells but did not react with uninfected mouse embryo cells or with cells infected by polyoma virus. DNA hybridization studies confirmed the presence of K virus DNA in KVBCG1B cells and suggested integration of the viral genome into host chromosomal DNA. K virus produces both persistent infection and cell transformation in glial cultures derived from its natural host.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-7-1253
1984-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/7/JV0650071253.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-7-1253&mimeType=html&fmt=ahah

References

  1. Bond S. B., Howley P. M., Takemoto K. K. 1978; Characterization of K virus and its comparison with polyoma virus. Journal of Virology 28:337–343
    [Google Scholar]
  2. Crawford L. V. 1969; Purification of polyoma virus. In Fundamental Techniques in Virology pp 75–81 Edited by Habel K., Salzman N. P. New York & London: Academic Press;
    [Google Scholar]
  3. Dörries K., Johnson R. T., Ter Meulen V. 1979; Detection of polyoma virus DNA in PML-brain tissue by (in situ) hybridization. Journal of General Virology 42:49–57
    [Google Scholar]
  4. Greenlee J. E. 1979; Pathogenesis of K virus infection in newborn mice. Infection and Immunity 26:705–713
    [Google Scholar]
  5. Greenlee J. E. 1981; Effect of host age on experimental K virus infection in mice. Infection and Immunity 33:297–303
    [Google Scholar]
  6. Greenlee J. E. 1983; Pathogenesis of K virus infection in mice. In Polyomaviruses and Human Neurological Diseases pp 325–342 Edited by Sever J. L., Madden D. L. New York: Alan R. Liss;
    [Google Scholar]
  7. Greenlee J. E., Narayan O., Johnson R. T., Herndon R. M. 1977; Induction of brain tumours in hamsters with BK virus, a human papovavirus. Laboratory Investigation 36:636–641
    [Google Scholar]
  8. Greenlee J. E., Dodd W. K., Oster-Granite M. L. 1982; An in vitro assay for K-papovavirus of mice. Journal of Virological Methods 4:139–146
    [Google Scholar]
  9. Gross-Bellard M., Oudet P., Le Chambon P. 1973; Isolation of high-molecular-weight DNA from mammalian cells. European Journal of Biochemistry 36:32–38
    [Google Scholar]
  10. Law M. F., Takemoto K. K., Howley P. M. 1979; Characterization of the genome of the murine papovavirus K. Journal of Virology 30:90–97
    [Google Scholar]
  11. Law M. F., Lowy D. R., Dvoretzky I., Howley P. M. 1981; Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences. Proceedings of the National Academy of Sciences, U,. S,. A 78:2727–2731
    [Google Scholar]
  12. Oster-Granite M. L., Narayan O., Johnson R. T., Herndon R. M. 1978; Studies of cultured human and simian fetal brain cells. II. Infections with human (BK) and simian (SV40) papovaviruses. Neuropathology and Applied Neurobiology 4:443–455
    [Google Scholar]
  13. Padgett B. L., Walker D. L. 1983; Virologic and serologic studies of progressive multifocal leukoencephalopathy. In Polyomaviruses and Human Neurological Diseases pp 107–117 Edited by Sever J. L., Madden D. L. New York: Alan R. Liss;
    [Google Scholar]
  14. Padgett B. L., Zu Rhein G. M., Walker D. L., Eckroade R. J. 1971; Cultivation of papova-like virus from human brain with progressive multifocal leukoencephalopathy. Lancet 1:1257–1260
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Shein H. M. 1967; Transformation of astrocytes and destruction of spongioblasts induced by a simian tumor virus (SV40) in cultures of human fetal neuroglia. Journal of Neuropathology and Experimental Neurology 26:60–76
    [Google Scholar]
  17. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  18. Takemoto K. K., Fabisch P. 1970; Transformation of mouse cells by K-papovavirus. Virology 40:135–143
    [Google Scholar]
  19. Takemoto K. K., Linke H., Miyamura T., Fareed G. C. 1979; Persistent BK papovavirus infection of transformed human fetal brain cells. I. Episomal viral DNA in cloned cell lines deficient in T-antigen expression. Journal of Virology 29:1177–1185
    [Google Scholar]
  20. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazo-benzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences, U,. S,. A 76:3683–3687
    [Google Scholar]
  21. Walker D. L. 1978; Progressive multifocal leukoencephalopathy. An opportunistic viral infection of the central nervous system. In Handbook of Clinical Neurology vol 34: pp 307–329 Edited by Vinken P. J., Bruyn G. W. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  22. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T. 1972; Further studies of a simian virus 40-like virus isolated from human brain. Journal of Virology 10:147–149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-7-1253
Loading
/content/journal/jgv/10.1099/0022-1317-65-7-1253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error