Seven species of dsRNA were isolated from plants infected with tomato bushy stunt virus (TBSV). One of these had a length of 4.8 kilobase pairs (kbp), corresponding to the replicative form dsRNA of TBSV genomic ssRNA; the other six were subgenomic RNA with lengths ranging from 3.3 to 0.44 kbp. Analyses of total and polysomal ssRNA from TBSV-infected tissue by gel transfer hybridization with cDNA probes prepared from genomic TBSV RNA revealed genomic RNA and nine shorter species of ssRNA. Six of the latter were shown to be artefacts probably attributable to the interaction between fragments of TBSV RNA and plant RNA. The remaining three had lengths of 1.8, 1.5 and 1.1 kbp and, since they were found in polysome preparations, may be TBSV subgenomic mRNA species; none of these corresponded exactly in length to any of the subgenomic dsRNA.

Keyword(s): cDNA , RNA, ds, subgenomic and TBSV

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