1887

Abstract

SUMMARY

The large glycoprotein, GP90, of respiratory syncytial virus (RSV) was purified by affinity chromatography using a monoclonal antibody. Hyperimmune rabbit antiserum directed specifically to the purified GP90 neutralized RSV to high titre but did not inhibit fusion of previously infected cells. I-labelled GP90 bound rapidly to HEp-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum.

Keyword(s): glycoprotein , purification and RSV
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1984-04-01
2021-10-19
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References

  1. Batteiger B., Newhall W. J., Jones R. B. 1982; The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. Journal of Immunological Methods 55:297–307
    [Google Scholar]
  2. Belshe R. B., Van Voris L. P., Mufson M. A. 1982; Parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. Journal of Infectious Diseases 145:311–319
    [Google Scholar]
  3. Bernstein J. M., Hruska J. F. 1981; Respiratory syncytial virus proteins: identification by immunoprecipition. Journal of Virology 38:278–285
    [Google Scholar]
  4. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  6. Delarco J., Todaro G. J. 1976; Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, GP71. Cell 8:365–371
    [Google Scholar]
  7. Dubovi E. J. 1982; Analysis of proteins synthesized in respiratory syncytial virus-infected cells. Journal of Virology 42:372–378
    [Google Scholar]
  8. Fernie B. F., Gerin J. L. 1980; The stabilization and purification of respiratory syncytial virus using MgS04. Virology 106:141–144
    [Google Scholar]
  9. Fernie B. F., Gerin J. L. 1982; Immunochemical identification of viral and nonviral proteins of respiratory syncytial virus virion. Infection and Immunity 37:243–249
    [Google Scholar]
  10. Fernie B. F., Cote P. J. Jr, Gerin J. L. 1982; Classification of hybridomas to respiratory syncytial virus glycoproteins. Proceedings of the Society for Experimental Biology and Medicine 171:266–271
    [Google Scholar]
  11. Fowler A. K., Twardzik D. R., Reed C. D., Weislow O. S., Hellman A. 1977; Binding characteristics of Rauscher leukemia virus envelope glycoprotein GP71 to murine lymphoid cells. Journal of Virology 24:729–735
    [Google Scholar]
  12. Fraker P. J., Speck J. C. 1978; Protein and cell membrane iodinations with a sparingly soluble chloramide, l, 3, 4, 6-tetrachloro-3α, 6α-diphenylglycouril. Biochemical and Biophysical Research Communications 80:849–857
    [Google Scholar]
  13. Henderson F. W., Collier A. M., Clyde W. A., Denny F. W. 1979; Respiratory syncytial virus infections, reinfections and immunity. New England Journal of Medicine 300:530–534
    [Google Scholar]
  14. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1969; Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
    [Google Scholar]
  15. Kim H. W., Arrobio J. O., Brandt C. D., Jeffries B. C., Pyles G., Reid J. L., Chanock R. M., Parrott R. H. 1973; Epidemiology of respiratory syncytial infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection. American Journal of Epidemiology 98:216–225
    [Google Scholar]
  16. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  17. Lonberg-Holm K., Phillipson L. 1974; Early interactions between animal viruses and cells. Monographs in Virology 9:1–148
    [Google Scholar]
  18. Markwell M. A., Paulson J. C. 1980; Sendai virus utilizes specific sialyloligosaccharides as host cell receptor determinants. Proceedings of the National Academy of Sciences, U. S. A 77:5693–5697
    [Google Scholar]
  19. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  20. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D., Murphy B., Brandt C., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington, D.C. II. Infection and disease with respect to age, immunologic status, race, and sex. American Journal of Epidemiology 98:289–300
    [Google Scholar]
  21. Paulson J. C., Sadler J. E., Hill R. L. 1979; Restoration of specific myxovirus receptors to asialoerythrocytes by incorporation of sialic acid with pure sialyltransferases. Journal of Biological Chemistry 254:2120–2124
    [Google Scholar]
  22. Peeples M., Levine S. 1979; Respiratory syncytial virus polypeptides: their location in the virion. Virology 95:137–145
    [Google Scholar]
  23. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus proteins. Association of both hemagglutination and neuraminidase activities with the larger SV5 glycoprotein. Virology 50:640–652
    [Google Scholar]
  24. Thimmig R. L., Hughes J. V., Kinders R. J., Milenkovic A. G., Johnson T. C. 1980; Isolation of the glycoprotein of vesicular stomatitis virus and its binding to cell surfaces. Journal of General Virology 50:279–291
    [Google Scholar]
  25. Walsh E. E., Hruska J. F. 1983; Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. Journal of Virology 47:171–177
    [Google Scholar]
  26. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1983; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
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