Increased yields of Sendai virus-induced human leukocyte interferon (IFN-α) were obtained by lowering the incubation temperature from the initial 37°C within 1 to 6 h after the addition of virus. Maximal stimulation was found when this was done 2 to 4 h after induction and when the lowered temperature was between 29 and 32°C. Compared to cultures kept at 37°C throughout, the resulting IFN-α yields were enhanced by 2.5- to 3-fold. This was mainly due to prolonged synthesis of IFN-α, which could be observed up to 20 h after induction, whereas in cultures kept at 37°C the production of IFN-α had practically ceased approx. 10 h after the addition of virus.

Keyword(s): IFN , leukocyte culture , temperature and yields

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