1887

Abstract

SUMMARY

We have established four murine hybridoma cell lines which secrete specific antibody to avian infectious bronchitis virus (IBV) strain Massachusetts M41. Two monoclonal antibodies reacted with the spike protein and two reacted with the membrane protein. The specificity of the monoclonal antibodies for the external structural proteins was detected by immunoprecipitations using radiolabelled virus. The reactions of the monoclonal antibodies showed that one (S1) of the two glycopolypeptides associated with the spike protein has a strain-specific region involved in neutralization and haemagglutination, and the membrane protein has antigenic determinants which are present on the three strains of IBV tested (M41, Beaudette and D41).

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1984-12-01
2024-04-26
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References

  1. Carter M. J., Willcocks M. M., Löffler S., Ter Meulen V. 1982; Relationships between monoclonal anti-body-binding sites on the measles virus haemagglutinin. Journal of General Virology 63:113–120
    [Google Scholar]
  2. Cavanagh D. 1981; Structural polypeptides of coronavirus IBV. Journal of General Virology 53:93–103
    [Google Scholar]
  3. Cavanagh D. 1983a; Coronavirus IBV: further evidence that the surface projections are associated with two glycol polypeptides. Journal of General Virology 64:1787–1791
    [Google Scholar]
  4. Cavanagh D. 1983b; Coronavirus IBV: structural characterization of the spike protein. Journal of GeneralVirology 64:2577–2583
    [Google Scholar]
  5. Cavanagh D., Darbyshire J. H., Davis P., Peters R. W. 1984; Induction of humoral neutralising and haemagglutination-inhibiting antibody by the spike protein of avian infectious bronchitis virus. Avian Pathology 13:573–583
    [Google Scholar]
  6. Collins A. R., Knobler R. L., Powell H., Buchmeier M. J. 1982; Monoclonal antibodies to murine hepatitisvirus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology 119:358–371
    [Google Scholar]
  7. Darbyshire J. H., Rowell J. G., Cook J. K. A., Peters R. W. 1979; Taxonomic studies on strains of avianinfectious bronchitis virus using neutralisation tests in tracheal organ cultures. Archives of Virology 61:227–238
    [Google Scholar]
  8. Fleming J. O., Stohlman S. A., Harmon R. C., Lai M. M. C., Frelinger J. A., Weiner L. P. 1983; Antigenicrelationships of murine coronaviruses: analysis using monoclonal antibodies to JHM (MHV-4) virus. Virology 131:296–307
    [Google Scholar]
  9. Heinz F. X., Berger R., Tuma W., Kunz C. 1983; Location of immunodominant antigenic determinants onfragments of the tick-borne encephalitis virus glycoprotein: evidence for two different mechanisms by whichantibodies mediate neutralization and haemagglutination inhibition. Virology 130:485–501
    [Google Scholar]
  10. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  11. Kouwenhoven B., Davelaar F. G., Burger A. G., Lutticken D. 1983; Significance of variant IB serotypes inthe Netherlands. In Disease Prevention and Control in Poultry Production (The International Union ofImmunological Societies, Proceedings No. 66), pp 53–71
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Mockett A. P. A., Darbyshire J. H. 1981; Comparative studies with an enzyme-linked immunosorbent assay (ELISA) for antibodies to avian infectious bronchitis virus. Avian Pathology 10:1–10
    [Google Scholar]
  14. Mockett A. P. A., Brown T. D. K., Davies P. J. 1984; A hybridoma secreting a neutralizing monoclonal antibodyto egg drop syndrome 1976 virus. Avian Pathology 13:333–337
    [Google Scholar]
  15. Nishikawa K., Isomura S., Suzuki S., Watanabe E., Hamaguchi M., Yoshida T., Nagai Y. 1983; Monoclonalantibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for straincomparisons. Virology 130:318–330
    [Google Scholar]
  16. Siddell S., Wege H., Ter Meulen V. 1983; The biology of coronaviruses. Journal of General Virology 64:761–776
    [Google Scholar]
  17. Sonza A., Breschkin A. M., Holmes I. H. 1984; The major surface glycoprotein of simian rotavirus (SA11) contains distinct epitopes. Virology 134318–327
    [Google Scholar]
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