1887

Abstract

SUMMARY

The morphological characteristics of murine cytomegalovirus replication in murine hepatocytes were investigated by electron microscopy and structural evidence of an unusual mode of virus maturation was detected. Nucleocapsids form in the nucleus; those with electron-dense cores bud into the perinuclear cisternae and acquire an outer envelope from the inner membrane of the nuclear envelope. Viral envelopes fuse with the outer membrane of the nuclear envelope releasing nucleocapsids in the paranuclear zone where they aggregate and form cytoplasmic inclusions. These inclusions are invariably surrounded by lamellae and vesicles of the Golgi complex into which nucleocapsids again bud. Virions, now covered with a new membrane envelope, are transported within secondary lysosomes and released by emiocytosis into the extracellular space.

Keyword(s): hepatitis , MCMV and ultrastructure
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-11-1979
1984-11-01
2022-08-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/11/JV0650111979.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-11-1979&mimeType=html&fmt=ahah

References

  1. Bennett J. M., Blume R. S., Wolff S. M. 1969; Characterization and significance of abnormal leucocyte granules in the beige mouse. A possible homologue for Chediak-Higashi Aleutian trait. Journal of Laboratory and Clinical Medicine 73:235–243
    [Google Scholar]
  2. Brodsky I., Rowe W. P. 1958; Chronic sublethal infection with mouse salivary gland virus. Proceedings of the Society for Experimental Biology and Medicine 99:654–655
    [Google Scholar]
  3. Carter A. R. 1968; Cytomegalovirus disease presenting as hepatitis. British Medical Journal iii 786
    [Google Scholar]
  4. Caul E. O., Clark S. K. R., Mott M. G., Perham T. G. M., Wilson R. S. E. 1971; Cytomegalovirus infections after open heart surgery. Lancet 1:777–781
    [Google Scholar]
  5. Craighead J. E. 1971; Pulmonary cytomegalovirus infection in the adult. American Journal of Pathology 63:487–504
    [Google Scholar]
  6. Craighead J. E., Hanshaw J. B., Carpenter C. B. 1967; Cytomegalovirus infection after renal allotransplantation. Journal of the American Medical Association 201:725–728
    [Google Scholar]
  7. De Marchi J. M., Kaplan A. S. 1977; Physiological state of human embryonic lung cells affects their response to human cytomegalovirus. Journal of Virology 23:126–132
    [Google Scholar]
  8. Diosi P., Rosin N. 1965; Cytomegalovirus infection. Association with hepatocytomegaly and abnormal liver function tests. New England Journal of Medicine 272:602–609
    [Google Scholar]
  9. Drew L. W., Mintz L., Milner R. C., Sands M., Ketterer B. 1981; Prevalence of cytomegalovirus infection in homosexual men. Journal of Infectious Diseases 143:188–191
    [Google Scholar]
  10. Duwall C. P., Casazza A. R., Grimley P. M., Carbone P. P., Rowe W. P. 1966; Recovery of cytomegalovirus from adults with neoplastic disease. Annals of Internal Medicine 64:531–541
    [Google Scholar]
  11. Fong C. K. Y. 1982; Ultrastructural localization of cytomegalovirus DNA synthesis in infected guinea-pig cells. Journal of General Virology 60:235–245
    [Google Scholar]
  12. Fong C. K. Y., Bia F., Hsiung G. D., Madore P., Chang P.-W. 1979; Ultrastructural development of guinea pig cytomegalovirus in cultured guinea pig embryo cells. Journal of General Virology 42:127–140
    [Google Scholar]
  13. Fong C. K. Y., Bia F., Hsiung G. D. 1980; Ultrastructural development and persistence of guinea pig cytomegalovirus in duct cells of guinea pig submaxillary gland. Archives of Virology 64:97–108
    [Google Scholar]
  14. Furukawa T. 1979; Cell cycle-dependent chronic infection of human cytomegalovirus in human osteogenic sarcoma cells. Journal of General Virology 45:81–88
    [Google Scholar]
  15. Grundy Chalmer J. E., Mackenzie J. S., Stanley N. F. 1981; Influence of H-2 and non-H-2 genes on resistance to murine cytomegalovirus infection. Infection and Immunity 32:277–286
    [Google Scholar]
  16. Gupta P., Rapp R. 1978; Cyclic synthesis of human cytomegalovirus induced proteins in infected cells. Virology 84:199–202
    [Google Scholar]
  17. Henson D. 1969; Cytomegalovirus hepatitis in an adult. An autopsy report. Archives of Pathology 88:199–203
    [Google Scholar]
  18. Henson D., Neapolitan C. 1970; Pathogenesis of chronic mouse cytomegalovirus infection in submaxillary glands of C3H mice. American Journal of Pathology 58:255–267
    [Google Scholar]
  19. Henson D., Strano A. J. 1972; Mouse cytomegalovirus. Necrosis of infected and morphologically normal submaxillary gland acinar cells during termination of chronic infection. American Journal of Pathology 68:183–202
    [Google Scholar]
  20. Henson D., Smith R. D., Gehrke I. 1966; Non fatal mouse cytomegalovirus hepatitis. American Journal of Pathology 49:871–888
    [Google Scholar]
  21. Hudson J. B., Misra V., Rosmann T. R. 1976; Properties of the multicapsid virions of murine cytomegalovirus. Virology 72:224–234
    [Google Scholar]
  22. Kääriäinen L., Paloheimo J., Klemola E., Makela T., Kiovunemi A. 1966; Cytomegalovirus-mononucleosis: isolation of the virus and demonstrations of subclinical infections after fresh blood transfusions in connection with open heart surgery. Annates medicinae experimentalis et biologiae Fenniae 44:297–301
    [Google Scholar]
  23. Kimball H. R., Ford G. H., Wolff S. M. 1975; Lysosomal enzymes in normal and Chediak-Higashi blood leukocytes. Journal of Laboratory and Clinical Medicine 86:616–630
    [Google Scholar]
  24. Klemola E., Kääriäinen L. 1965; Cytomegalovirus as a possible cause of a disease resembling infectious mononucleosis. Finnish Medical Journal 2:1099–1102
    [Google Scholar]
  25. Klemola E., Von Easen R., Wager O., Haltia K., Kiovunemi A., Salmi I. 1969; Cytomegalovirus mononucleosis in previously healthy individuals. Five new cases and follow-up of 13 previously published cases. Annals of Internal Medicine 71:11–19
    [Google Scholar]
  26. Lang D. J., Scolnick E. M., Willerson J. T. 1968; Association of cytomegalovirus infection with the post-perfusion syndrome. New England Journal of Medicine 278:1147–1149
    [Google Scholar]
  27. Larson C. H. 1976; Murine cytomegalovirus glomerulonephritis: clinical and ultrastructural observations. American Journal of Veterinary Research 376:823–830
    [Google Scholar]
  28. Lussier G., Berthiaume L., Payment P. 1974; Electron microscopy of murine cytomegalovirus: development of the virus in vivo and in vitro. Archives of Virology 46:269–280
    [Google Scholar]
  29. Lutzner M. A., Lowrie C. T., Jordan H. W. 1967; Giant granules in leukocytes of the beige mouse. Journal of Heredity 58:299–300
    [Google Scholar]
  30. McCordock H. A., Smith M. G. 1936; The visceral lesions produced in mice by the salivary gland virus of mice. Journal of Experimental Medicine 36:303–310
    [Google Scholar]
  31. McGavran M. H., Smith M. G. 1965; Ultrastructural, cytochemical and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture. Experimental and Molecular Pathology 4:1–10
    [Google Scholar]
  32. Mannini A., Medearis D. N. 1961; Mouse salivary gland virus infections. American Journal of Hygiene 73:329–343
    [Google Scholar]
  33. Medearis D. N. 1964; Mouse cytomegalovirus infection. II. Observations during prolonged infection. American Journal of Hygiene 80:103–112
    [Google Scholar]
  34. Middlekamp J. N., Patrizi G., Reed C. A. 1967; Light and electron microscopic studies of the guinea pig cytomegalovirus. Journal of Ultrastructure Research 18:85–101
    [Google Scholar]
  35. Mildvan D., Mathur V., Enlow R. W., Romain P. L., Winchester R. J., Colp C., Singman H., Adelsberg B. R., Spigland I. 1982; Opportunistic infections and immune deficiency in homosexual men. Annals of Internal Medicine 96:700–704
    [Google Scholar]
  36. Muller M., Misra V., Chantler J. K., Hudson J. B. 1978; Murine cytomegalovirus gene expression during non productive infection in G0 phase 3T3 cells. Virology 90:279–287
    [Google Scholar]
  37. Olding L. B., Kingsbury D. T., Oldstone M. B. A. 1976; Pathogenesis of cytomegalovirus infection. Distribution of virus products, immune complexes and autoimmunity during latent murine infection. Journal of General Virology 33:267–280
    [Google Scholar]
  38. Papadimitriou J. M., Shellam G. R. 1981; A cytophotometric measurement of DNA in murine hepatocytic nuclei during cytomegalovirus infection. Histochemistry 72:481–487
    [Google Scholar]
  39. Papadimitriou J. M., Shellam G. R., Allan J. E. 1982; The effect of the beige mutation on infection with murine cytomegalovirus. Histopathologic studies. American Journal of Pathology 108:299–309
    [Google Scholar]
  40. Prieur D. J., Davis W. C., Padgett G. A. 1972; Defective function of renal lysosomes in mice with the Chediak-Higashi syndrome. American Journal of Pathology 67:227–240
    [Google Scholar]
  41. Reynolds D. W., Stagno S., Hosty T. S., Tiller M., Alford C. A. Jr 1973; Maternal cytomegalovirus excretion and perinatal infection. New England Journal of Medicine 289:1–5
    [Google Scholar]
  42. Reynolds D. W., Stagno S., Stubbs G., Dahle A. J., Livingston M. M., Saxon S. S., Alford C. A. Jr 1974; Inapparent congenital cytomegalovirus infection with elevated cord IgM levels. Causal relationship with auditory and mental deficiency. New England Journal of Medicine 290:291–296
    [Google Scholar]
  43. Rinaldo C. R., Carney W. P., Richter B. S., Black P. H., Hirsch M. S. 1980; Mechanisms of immuno-suppression in cytomegaloviral mononucleosis. Journal of Infectious Diseases 141:485–490
    [Google Scholar]
  44. Rowe W. P., Hartley J. W., Waterman S., Turner H. C., Huebner R. J. 1956; Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proceedings of the Society for Experimental Biology and Medicine 92:418–424
    [Google Scholar]
  45. Ruebner B. H., Miyai K., Slusser R. J., Wedemyer P., Medearis D. N. 1964; Mouse cytomegalovirus infection. An electron microscopic study of hepatic parenchymal cells. American Journal of Pathology 44:799–821
    [Google Scholar]
  46. Ruebner B. H., Hirano T., Slusser R. J., Medearis D. N. 1965; Human cytomegalovirus infection. Electron microscopic and histochemical changes in cultures of human fibroblasts. American Journal of Pathology 46:477–496
    [Google Scholar]
  47. Ruebner B. H., Hirano T., Slusser R., Osborn J., Medearis D. N. 1966; Cytomegalovirus infection. Viral ultra-structure with particular reference to the relationship of lysosomes to cytoplasmic inclusions. American Journal of Pathology 48:971–979
    [Google Scholar]
  48. Shellam G. R., Allan J. E., Papadimitriou J. M., Bancroft G. J. 1981; Increased susceptibility to cytomegalovirus infections in beige mutant mice. Proceedings of the National Academy of Sciences, U,. S,. A 78:481–487
    [Google Scholar]
  49. Smith D. R., Wehner R. W. 1980; Acute cytomegalovirus glomerulonephirits. An experimental model. Laboratory Investigation 43:278–286
    [Google Scholar]
  50. Smith M. G. 1954; Propagation of salivary gland virus of the mouse in tissue cultures. Proceedings of the Society for Experimental Biology and Medicine 86:435–440
    [Google Scholar]
  51. Smith M. G. 1959; The salivary gland viruses of man and animals (cytomegalic inclusion disease). Progress in Medical Virology 2:171–202
    [Google Scholar]
  52. Spencer E. S. 1974; Clinical aspects of cytomegalovirus infection in kidney graft recipients. Scandinavian Journal of Infectious Diseases 6:315–323
    [Google Scholar]
  53. Stern H., Elek S. K., Both J. C., Fleek D. G. 1969; Microbial cause of mental retardation. The role of prenatal infection with cytomegalovirus, rubella virus and toxoplasma. Lancet ii:443–448
    [Google Scholar]
  54. Vassalli J. D., Granelli-Piperno A., Griscelli C., Reich E. 1978; Specific protease deficiency in polymorpho-nuclear leukocytes of Chediak-Higashi syndrome and beige mice. Journal of Experimental Medicine 147:1285–1290
    [Google Scholar]
  55. Weller T. H. 1971a; The cytomegaloviruses, ubiquitous agents with protean clinical manifestations (part one). New England Journal of Medicine 285:203–214
    [Google Scholar]
  56. Weller T. H. 1971b; The cytomegaloviruses, ubiquitous agents with protean clinical manifestations (part two). New England Journal of Medicine 285:267–274
    [Google Scholar]
  57. Weller T. H., Macaulay J. C., Craig J. M., Wirth P. 1957; Isolation of intranuclear inclusion-producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proceedings of the Society for Experimental Biology and Medicine 94:4–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-11-1979
Loading
/content/journal/jgv/10.1099/0022-1317-65-11-1979
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error