1887

Journal of General Virology header logo

Volume 65, Issue 10

Satellite Nature of the Viroid-like RNA-2 of Solanum Nodiflorum Mottle Virus and the Ability of Other Plant Viruses to Support the Replication of Viroid-like RNA Molecules Free

Abstract

SUMMARY

The stock culture of solanum nodiflorum mottle virus (SNMV) was freed from RNA-2 by passage through single lesions induced in inoculated leaves of by dilute inocula of partially purified SNMV RNA-1. This isolate (S1) was indistinguishable serologically and in symptomatology from the stock culture of SNMV. Using a sensitive bioassay for detecting RNA-2, no SNMV RNA-2 was found in RNA from particles of the S1 isolate purified after three successive passages in . Addition of SNMV RNA-2 (which is not infective alone) had little or no effect on the infectivity of S1 RNA but restored the RNA content of particles to that found in the stock culture (RNA-1 and RNA-2). However, S1 did not support the replication of the RNA-2 from lucerne transient streak virus (LTSV). The possible interaction of the RNA-2 species from LTSV and SNMV with the genomes of other plant viruses was tested by mixing the RNA-2 species with nucleic acid from 11 other viruses. The survival of RNA-2 activity on inoculated leaves for up to 15 days complicated the interpretation of the results of these tests but a small amount of LTSV RNA-2 and SNMV RNA-2 was obtained only from particles of turnip rosette virus purified from plants inoculated with the relevant RNA mixtures. These and other data suggest that these RNA-2 molecules with physicochemical properties similar to viroids found in particles of some sobemoviruses are satellite RNA species whose multiplication is assisted by some, but not all, of the viruses in this group.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): circular RNA , LTSV , satellite-like RNA , SNMV and viroid-like RNA
© Journal of General Virology 1984
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-10-1713
1984-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/10/JV0650101713.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-10-1713&mimeType=html&fmt=ahah

References

  1. Diener T. O. 1979 Viroids and Viroid Diseases New York: John Wiley & Sons;
     [Google Scholar]
  2. Francki R. I. B., Chu P. W. G., Keese P. K. 1983a; The satellite nature of a viroid-like RNA from lucerne transient streak virus. In Plant Infectious Agents pp 175–180 Edited by Robertson H. D., Howell S. H., Zaitlin M., Malmberg R. L. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  3. Francki R. I. B., Randles J. W., Hatta T., Davies C., Chu P. W. G., Mclean G. D. 1983b; Subterranean clover mottle virus: another virus from Australia with encapsidated viroid-like RNA. Plant Pathology 32:47–59
     [Google Scholar]
  4. Gould A. R., Hatta T. 1981; Studies on encapsidated viroid-like RNA. III. Comparative studies on RNAs isolated from velvet tobacco mottle virus and Solanum nodiflorum mottle virus. Virology 109:137–147
     [Google Scholar]
  5. Gould A. R., Francki R. I. B., Randles J. W. 1981; Studies on encapsidated viroid-like RNA. IV. Requirement for infectivity and specificity of two RNA components from velvet tobacco mottle virus. Virology 110:420–426
     [Google Scholar]
  6. Greber R. S. 1981; Some characteristics of solanum nodiflorum mottle virus - a beetle-transmitted isometric virus from Australia. Australian Journal of Biological Sciences 34:369–378
     [Google Scholar]
  7. Haseloff J., Symons R. H. 1982; Comparative sequence and structure of viroid-like RNAs of two plant viruses. Nucleic Acids Research 10:3681–3691
     [Google Scholar]
  8. Hollings M., Stone O. 1973; Turnip rosette virus. Commonwealth Mycological Institute/ Association of Applied Biologists Descriptions of Plant Viruses no. 125
    [Google Scholar]
  9. Jacquemond M., Lot H. 1981; L’ARN satellite du virus de la mosaique du concombre. I. Comparison de l’aptitude a induire la nécrose de la tomate d’ARN isolés de plusieurs souches du virus. Agronomie 1:927–932
     [Google Scholar]
  10. Jones A. T. 1972; Purification and properties of elderberry latent virus, one of four sap-transmissible viruses obtained from American elder (Sambucus canadensis L.). Annals of Applied Biology 70:49–58
     [Google Scholar]
  11. Jones A. T., Mayo M. A. 1983; Interaction of lucerne transient streak virus and the viroid-like RNA-2 of Solanum nodiflorum mottle virus. Journal of General Virology 64:1771–1774
     [Google Scholar]
  12. Jones A. T., Mayo M. A., Duncan G. H. 1983; Satellite-like properties of small circular RNA molecules in particles of lucerne transient streak virus. Journal of General Virology 64:1167–1173
     [Google Scholar]
  13. Keese P., Bruening G., Symons R. H. 1984; Comparative sequence and structure of circular RNAs from two isolates of lucerne transient streak virus. FEBS Letters (in press)
    [Google Scholar]
  14. Mossop D. W., Francki R. I. B. 1979; Comparative studies on two satellite RNAs of cucumber mosaic virus. Virology 95:395–404
     [Google Scholar]
  15. Murant A. F., Mayo M. A. 1982; Satellites of plant viruses. Annual Review of Phytopathology 20:49–70
     [Google Scholar]
  16. Paliwal Y. C. 1984; Interaction of the viroid-like RNA-2 of lucerne transient streak virus with southern bean mosaic virus. Canadian Journal of Plant Pathology 6: (in press)
    [Google Scholar]
  17. Randles W., Davies C., Hatta T., Gould A. R., Francki R. I. B. 1981; Studies on encapsidated viroid-like RNA. I. Characterization of velvet tobacco mottle virus. Virology 108:111–122
     [Google Scholar]
  18. Randles J. W., Steger G., Riesner D. 1982; Structural transitions in viroid-like RNAs associated with cadang-cadang disease, velvet tobacco mottle, and Solanum nodiflorum mottle virus. Nucleic Acids Research 10:5569–5586
     [Google Scholar]
  19. S–chneider I.R. 1969; Satellite-like particle of tobacco ringspot virus that resembles tobacco ringspot virus. Science 166:1627–1629
     [Google Scholar]
  20. Tien-Po K., Davies C., Hatta T., Francki R. I. B. 1981; Viroid-like RNA encapsidated in lucerne transient streak virus. FEBS Letters 132:353–356
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-10-1713
Loading
Satellite Nature of the Viroid-like RNA-2 of Solanum Nodiflorum Mottle Virus and the Ability of Other Plant Viruses to Support the Replication of Viroid-like RNA Molecules
J. Gen. Virol. 65, 1713 (1984); https://doi.org/10.1099/0022-1317-65-10-1713
/content/journal/jgv/10.1099/0022-1317-65-10-1713
/content/journal/jgv/10.1099/0022-1317-65-10-1713
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error