1887

Abstract

SUMMARY

Sixteen monoclonal antibodies that reacted with the envelope glycoprotein (E) of 17D vaccine strain yellow fever virus (17D YF), including two antibodies produced against dengue 2 virus, were used in a solid phase competitive binding assay (CBA) to define spatial relationships among antigenic determinants on 17D YF E. The antibodies showed YF strain, type or flavivirus group specificities and nine epitopes were identified on 17D YF E by patterns of neutralization, haemagglutination inhibition and competition of antibody binding. Epitopes defined by neutralizing antibodies with strain and type specificities appeared spatially distant but competition between type-specific neutralizing antibodies and some non-neutralizing antibodies against type and group determinants suggested close proximity among epitopes in these regions. Despite competition between some neutralizing and non-neutralizing monoclonal antibodies in CBA, plaque assays revealed no interference with neutralization by non-neutralizing antibody.

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1984-10-01
2024-11-08
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References

  1. Clarke D. H. 1960; Antigenic analysis of certain group B arthropod-borne viruses by antibody absorption. Journal of Experimental Medicine 111:21–32
    [Google Scholar]
  2. Ehrlich P. H., Moyle W. R., Moustafa Z. A. 1983; Further characterization of cooperative interactions of monoclonal antibodies. Journal of Immunology 131:1906–1912
    [Google Scholar]
  3. Ey P. L., Prowse S. J., Jenkins C. R. 1978; Isolation of pure IgG1 IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  4. Fraker P. J., Speck J. C. 1978; Protein and cell membrane iodinations with a sparingly soluble chloroamide, l, 3, 4, 6-tetrachloro-3a, 6a-diphenylglycouril. Biochemical and Biophysical Research Communications 80:849–857
    [Google Scholar]
  5. Frankel M. E., Gerhard W. 1979; The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus. Molecular Immunology 16:101–105
    [Google Scholar]
  6. Gentry M. K., Henchal E. A., McCown J. M., Brandt W. E., Dalrymple J. M. 1982; Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 31:830–836
    [Google Scholar]
  7. Gerhard W., Yewdell J., Frankel M. 1980; An experimental approach to define the antigenic structures of the haemagglutinin molecule of A/PR/8/34. In Structure and Variation in Influenza Virus pp 273–282 Edited by Laver W. G., Air G. M. New York: Elsevier/North-Holland;
    [Google Scholar]
  8. Heinz F. X., Berger R., Tuma W., Kunz C. 1983; A topological and functional model of epitopes on the structural glycoprotein of tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126:525–537
    [Google Scholar]
  9. Kimura-Kiroda J., Yasui K. 1983; Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus, using monoclonal antibodies. Journal of Virology 45:124–132
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Madrid A. T. De, Porterfield J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
    [Google Scholar]
  12. Massey R. J., Schochetman G. 1981; Viral epitopes and monoclonal antibodies: isolation of blocking antibodies that inhibit virus neutralization. Science 213:447–449
    [Google Scholar]
  13. Meurman O. 1983; Detection of antiviral IgM antibodies and its problems - a review. In New Developments in Diagnostic Virology pp 111–113 Edited by Bachmann P. A. Wien: Springer-Verlag;
    [Google Scholar]
  14. Nishikawa K., Isomura S., Suzuki S., Watanabe E., Hamaguchi M., Yoshida T., Nagai Y. 1983; Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparisons. Virology 130:318–330
    [Google Scholar]
  15. Peiris J. S. M., Porterfield J. S., Roehrig J. T. 1982; Monoclonal antibodies against the flavivirus West Nile. Journal of General Virology 58:283–289
    [Google Scholar]
  16. Price W. H., Casals J., Thind I., O’Leary W. 1973; Sequential immunization procedure against group B arboviruses using living attenuated 17D yellow fever virus, living attenuated Langat E5 virus, and living attenuated dengue 2 virus (New Guinea C isolate). American Journal of Tropical Medicine and Hygiene 22:509–523
    [Google Scholar]
  17. Roehrig J. T., Mathews J. H., Trent D. W. 1983; Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies. Virology 128:118–126
    [Google Scholar]
  18. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  19. Schmaljohn A. L., Kokubun K. M., Cole G. A. 1983; Protective monoclonal antibodies define maturational and pH-dependent antigenic changes in Sindbis virus El glycoprotein. Virology 130:144–154
    [Google Scholar]
  20. Scott R. McN., Eckels K. H., Bancroft W. H., Summers P. L., McCown J. M., Anderson J. H., Russell P. K. 1983; Dengue 2 vaccine: dose response in volunteers in relation to yellow fever immune status. Journal of Infectious Diseases 148:1055–1060
    [Google Scholar]
  21. Shope R. E., Sather G. E. 1979; Arboviruses. In Diagnostic Procedures for Viral Rickettsial and Chlamydial Infections pp 767–814 Edited by Lennette E. H., Schmidt N. J. Washington, D,. C.: American Public Health Association;
    [Google Scholar]
  22. Stone M. R., Nowinski R. C. 1980; Topological mapping of murine leukemia virus proteins by competitionbinding assays with monoclonal antibodies. Virology 100:370–381
    [Google Scholar]
  23. Trent D. W. 1977; Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. Journal of Virology 22:608–618
    [Google Scholar]
  24. Trent D. W., Qureshi A. A. 1971; Structural and nonstructural proteins of Saint Louis encephalitis virus. Journal of Virology 7:379–388
    [Google Scholar]
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