1887

Abstract

Summary

The involvement of lysosomes in infection by mouse hepatitis virus 3 (MHV) was studied. L cells were infected with MHV in the presence of NHCl or chloroquine, weak bases which increase the intralysosomal pH and impair lysosomal functions. NHCl significantly inhibited virus-induced cytopathic effects and MHV replication, but did not prevent the attachment of H-labelled virus. No inhibition of MHV replication by NHCl and chloroquine was observed when lysosomotropic agents were added later than 3 h post-infection, suggesting the direct involvement of lysosomes in release of the viral genome into cytoplasm. These results, together with the lack of antibody-mediated immune lysis of MHV-infected cells, suggest that MHV entered cells by an endocytic pathway (viropexis) followed by internalization into cellular lysosomes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-1-227
1984-01-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-1-227
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error