1887

Abstract

SUMMARY

Heterotypic complementation between temperature-sensitive () mutants of Sindbis (SIN) and Western equine encephalitis (WEE) viruses occurs under appropriate conditions. One heterotypic pair, SIN 153 × WEE 39 showed efficient complementation, and four other combinations gave detectable complementation, indicating that these two viruses, which are closely related serologically and biochemically, are sufficiently closely related to complement each other functionally. Cells mixedly infected with mutants or wild-type strains of both SIN and WEE viruses produced phenotypically mixed virions, in addition to both parental viruses. Various types of phenotypically mixed virions have been identified by neutralization with corresponding antisera, by thermal inactivation and by temperature sensitivity of replication. Some virions contained WEE genomes and envelopes containing primarily SIN proteins. Other phenotypically mixed virus yields contained primarily doubly neutralizable viruses which are presumed to have a mosaic of envelope proteins. Phenotypically mixed virions were morphologically indistinguishable from the parental types.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1581
1983-07-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071581.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1581&mimeType=html&fmt=ahah

References

  1. Bell J. R., Bond M. W., Hunkapiller M. W., Strauss E. G., Strauss J. H., Yamamoto K., Simizu B. 1983; Structural proteins of Western equine encephalitis virus: amino acid compositions and N-terminal sequences. Journal of Virology 45:708–714
    [Google Scholar]
  2. Birdwell C. R., Strauss J. H. 1974; Replication of Sindbis virus. IV. Electron microscope study of the insertion of viral glycoproteins into the surface of infected chick cells. Journal of Virology 14:366–374
    [Google Scholar]
  3. Brown D. T. 1980; The assembly of alphaviruses. In The Togaviruses; Biology, Structure, Replication pp 473–500 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  4. Burge B. W., Pfefferkorn E. R. 1966a; Isolation and characterization of conditional lethal mutants of Sindbis virus. Virology 30:204–213
    [Google Scholar]
  5. Burge B. W., Pfefferkorn E. R. 1966b; Complementation between temperature-sensitive mutants of Sindbis virus. Virology 30:214–223
    [Google Scholar]
  6. Burge B. W., Pfefferkorn E. R. 1966c; Phenotypic mixing between group A arboviruses. Nature, London 210:1397–1399
    [Google Scholar]
  7. Calisher C. H., Shope R. E., Brandt W., Casals J., Karabatsos N., Murphy F. A., Tesh R. B., Wiebe M. E. 1980; Proposed antigenic classification of registered arboviruses. I. Togaviridae, Alphavirus. Intervirology 14:229–232
    [Google Scholar]
  8. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gel with water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  9. Dalrymple J. M., Schlesinger S., Russell R. K. 1976; Antigenic characterization of two Sindbis envelope glycoproteins separated by isoelectric focusing. Virology 69:93–103
    [Google Scholar]
  10. Garoff H., Frischauf A.-M., Simons K., Lehrach H., Delius H. 1980; The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino terminal region. Proceedings of the National Academy of Sciences, U. S. A 77:6376–6380
    [Google Scholar]
  11. Hashimoto K., Simizu B. 1978; Isolation and preliminary characterization of temperature-sensitive mutants of Western equine encephalitis virus. Virology 84:540–543
    [Google Scholar]
  12. Hashimoto K., Simizu B. 1982; A temperature-sensitive mutant of Western equine encephalitis virus with an altered envelope protein El and a defect in the transport of envelope glycoproteins. Virology 119:276–287
    [Google Scholar]
  13. Ishida I., Simizu B. 1981; Evidence for the presence of the minor capsid protein of Western equine encephalitis virus. Archives of Virology 67:159–164
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Lagwinska E., Stewart C. C., Adles C., Schlesinger S. 1975; Replication of lactic dehydrogenase virus and Sindbis virus in mouse peritoneal macrophages. Induction of interferon and phenotypic mixing. Virology 65:204–214
    [Google Scholar]
  16. Maeda S., Hashimoto K., Simizu B. 1979; Complementation between temperature-sensitive mutants isolated from Aedes albopictus cells persistently infected with Western equine encephalitis virus. Virology 92:532–541
    [Google Scholar]
  17. Ou J. H., Trent D. W., Strauss J. H. 1982; The 3′ non-coding regions of alphavirus RNAs contain repeatingsequences. Journal of Molecular Biology 156:719–730
    [Google Scholar]
  18. Porterfield J. S. 1980; Antigenic characteristics and classification of togaviridae. In The Togaviruses: Biology, Structure, Replication pp 13–46 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  19. Pringle C. R. 1977; Genetics of rhabdoviruses. In Comprehensive Virology vol 9: pp 239–289 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  20. Rice C. M., Strauss J. H. 1981; Nucleotide sequence of the 26S mRNA of Sindbis virus and the deduced sequence of the encoded virus structural proteins. Proceedings of the National Academy of Sciences, U. S. A 78:2062–2066
    [Google Scholar]
  21. Rice C. M., Strauss J. H. 1982; Association of Sindbis virion glycoproteins and their precursors. Journal of Molecular Biology 154:325–348
    [Google Scholar]
  22. Scupham R. K., Jones K. J., Sagik B. P., Bose H. R. Jr 1977; Virus-directed post-translational cleavage in Sindbis virus-infected cells. Journal of Virology 22:568–571
    [Google Scholar]
  23. Simizu B., YamaZaki S., Suzuki K., Terasima T. 1973; Gamma ray-induced small plaque mutants of Western equine encephalitis virus. Journal of Virology 12:1568–1578
    [Google Scholar]
  24. Simons K., Garoff H. 1980; The budding mechanisms of enveloped animal viruses. Journal of General Virology 50:1–21
    [Google Scholar]
  25. Strauss E. G., Strauss J. H. 1980; Mutants of alphaviruses: genetics and physiology. In The Togaviruses: Biology, Structure, Replication pp 393–426 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  26. Strauss E. G., Lenches E. M., Strauss J. H. 1976; Mutants of Sindbis virus. 1. Isolation and partial characterization of 89 new temperature-sensitive mutants. Virology 74:154–168
    [Google Scholar]
  27. Strauss J. H., Strauss E. G. 1977; Togaviruses. In The Molecular Biology of Animal Viruses pp 111–116 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  28. Ulmanen I., Söderlund H., Kääriäinen L. 1979; Role of protein synthesis in the assembly of Semliki Forest virus nucleocapsid. Virology 99:265–276
    [Google Scholar]
  29. Závadova A., Závada J., Weiss R. A. 1977; Unilateral phenotypic mixing of envelope antigens between togaviruses and vesicular stomatitis virus or avian RNA tumour virus. Journal of General Virology 37:557–567
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1581
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1581
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error