1887

Abstract

SUMMARY

Different methods of molecular hybridization were used to study DNA sequences of the highly oncogenic simian adenovirus SA7 (C8) present in the genomes of two transformed rat cell lines and in cells from three hamster tumours induced by adenovirus SA7. The entire DNA or the left-hand terminal I C fragment (19.5% of the genome) were employed. All cell lines retained an intact left-hand region of the SA7 genome (0 to 12.4 map units). The blot hybridization technique failed to detect any site specificity of integration of SA7 DNA into the cell genome. In all cell lines the expression of the II D fragment (1.8 to 10 map units) of SA7 DNA was observed. As judged by the patterns of integration of virus sequences into the cell genome, the highly oncogenic simian adenovirus SA7 (C8) is similar to the non-oncogenic human adenoviruses of group C, and is different from the highly oncogenic human adenovirus type 12.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-6-1381
1983-06-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/6/JV0640061381.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-6-1381&mimeType=html&fmt=ahah

References

  1. Alstein A. D., Sarycheva D. F., Dodonova N. N. 1967; Transforming activity of green monkey SA7 (C8) adeno-virus in tissue culture. Science 158:1455–1456
    [Google Scholar]
  2. Astrin S. M. 1978; Endogenous viral genes of the white Leghorn chicken: common site of residence and sites associated with specific phenotypes of viral gene expression. Proceedings of the National Academy of Sciences, U. S. A 75:5941–5945
    [Google Scholar]
  3. Doerfler W., Stabel S., Ibelgaufts H., Sutter D., Neumann R., Groneberg J., Scheidtmann K. H., Deuring R., Winterhoff U. 1980; Selectivity in integration sites of adenoviral DNA. Cold Spring Harbor Symposia on Quantitative Biology 44:551–564
    [Google Scholar]
  4. Fanning E., Doerfler W. 1976; Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12. Journal of Virology 20:373–383
    [Google Scholar]
  5. Flint S. J., Gallimore P. H., Sharp P. A. 1975; Adenovirus transcription. III. Comparison of viral RNA sequences in adenovirus 2 transformed and lytically infected cells. Journal of Molecular Biology 96:47–68
    [Google Scholar]
  6. Gallimore P. H. 1974; Interactions of adenovirus type 2 with rat embryo cells. Permissiveness, transformation and in vitro characteristics of adenovirus transformed rat embryo cells. Journal of General Virology 25:263–273
    [Google Scholar]
  7. Gallimore P. H., Sharp P. A., Sambrook J. 1974; Viral DNA in transformed cells. II. A study of sequences of adenovirus 2 DNA in nine lines of transformed rat cells using specific fragments of the viral genomes. Journal of Molecular Biology 89:49–55
    [Google Scholar]
  8. Gavrilov V. I., Kostiukov M. A., Bliumkin V. N., Zmiyeva R. G., Dreizin R. S. 1966; Chronic infection of a continuous swine kidney cell line (Res) due to monkey adenovirus type 12. Voprosy virusologii 1:43–47
    [Google Scholar]
  9. Gelb L. D., Kohne D. E., Martin M. A. 1971; Quantitation of simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. Journal of Molecular Biology 57:129–145
    [Google Scholar]
  10. Graham F. L., van Der Eb A. B. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  11. Kraiselburd E., Gage L. P., Weissbach A. 1975; Presence of a herpes simplex virus DNA fragment in a L cell clone obtained after infection with irradiated herpes simplex virus 1. Journal of Molecular Biology 97:533–542
    [Google Scholar]
  12. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the rightward operator of phage λ. Proceedings of the National Academy of Sciences, U. S. A 72:1184–1188
    [Google Scholar]
  13. Naroditsky B. S., Zavizion B. A., Karamov E. V., Chaplygina N. M., Dreizin R. S., Zolotarskaya E. F., Tikchonenko T. I. 1978; Fragmentation of simian adenovirus SA7 DNA with specific endonucleases SaiI and BamHI. Voprosy virusologii 1:36–41
    [Google Scholar]
  14. Ogino T., Takahashi M. 1970; Infection of simian adenovirus SA7 in hamster kidney and embryo cells. Biken Journal 13:303–312
    [Google Scholar]
  15. Ponomareva T. I., Tiunnikov G. I., Grodnitskaya N. A., Chaplygina N. M., Dreizin R. S., Kalinina T. I. 1981; The study of phenotypic features of cell lines transformed by the genome of oncogenic and non-oncogenic adenovirus. In Viruses of Cancer and Leukosis pp 106–109 Edited by Zhdanov V. M. Moscow: Institute of Virology, Academy of Medical Sciences of the U.S.S.R;
    [Google Scholar]
  16. Sambrook J., Botchan M., Gallimore P., Ozanne B., Pettersson U., Williams I., Sharp P. A. 1974; Viral DN A sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harbor Symposia on Quantitative Biology 39:615–632
    [Google Scholar]
  17. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  18. Stabel S., Doerfler R. P., Afriis W. 1980; Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. Journal of Virology 36:22–40
    [Google Scholar]
  19. Weinberg R. A. 1980; Integrated genomes of animal viruses. Annual Review of Biochemistry 49:197–226
    [Google Scholar]
  20. Zavizion B. A., Goldberg E. Z., Naroditsky B. S., Zolotarskaya E. E., Tikchonenko T. I. 1979; Fragmentation of simian adenovirus SA7 DNA by specific endonucleases R BglII and R HindIII. Voprosy virusologii 6:595–599
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-6-1381
Loading
/content/journal/jgv/10.1099/0022-1317-64-6-1381
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error