1887

Abstract

SUMMARY

The genomes of diabetogenic and non-diabetogenic variants of encephalomyocarditis virus were analysed by nucleic acid hybridization and RNA fingerprinting. Hybridization and thermal elution profiles failed to show any difference between the RNAs of the two variants, whereas fingerprinting of the T1-digested RNAs revealed at least one oligonucleotide, 20 to 25 nucleotides long, missing in the non-diabetogenic variant.

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/content/journal/jgv/10.1099/0022-1317-64-4-947
1983-04-01
2022-01-23
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References

  1. Aulakh G. S., Gallo R. C. 1977; Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hematopoietic neoplasias and absence in DNA from other tissues. Proceedings of the National Academy of Sciences, U. S. A 74:353–357
    [Google Scholar]
  2. Dewachter R., Fiers W. 1972; Preparative two dimensional polyacrylamide gel electrophoresis of 32P-labelled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  3. Kacian D. L., Myers J. C. 1976; Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities. Proceedings of the National Academy of Sciences, U. S. A 73:2191–2195
    [Google Scholar]
  4. Mcclintock P. R., Billups L. C., Notkins A. L. 1980; Receptors for encephalomyocarditis virus on murine and human cells. Virology 106:261–272
    [Google Scholar]
  5. Minor P. D., Schild G. C., Ferguson M., Mackay A., Magrath D. L., John A., Yates P. J., Spitz M. 1982; Genetic and antigenic variation in type 3 polioviruses: characterization of strains by monoclonal antibodies and Tl oligonucleotide mapping. Journal of General Virology 61:157–165
    [Google Scholar]
  6. Morishima T., Mcclintock P. R., Aulakh G. S., Billups L. C., Notkins A. L. 1982; Genomic and receptor attachment differences between Mengovirus and encephalomyocarditis virus. Virology (in press)
    [Google Scholar]
  7. Notkins A. L. 1977; Virus-induced diabetes mellitus. Archives of Virology 54:1–17
    [Google Scholar]
  8. Payvar F., Schimke R. T. 1979; Methyl-mercury hydroxide enhancement of translation and transcription of ovalbumin and conalbumin mRNAs. Journal of Biological Chemistry 254:7636–7642
    [Google Scholar]
  9. Pederson F. S., Haseltine W. A. 1980; A micromethod for detailed characterization of high molecular weight RNA. Methods in Enzymology 65:680–687
    [Google Scholar]
  10. Robson K. J. H., Doel T. R., Gorman B. M., Brown F. 1980; Biochemical aspects of variation in foot-and-mouth disease virus. Journal of General Virology 46:179–193
    [Google Scholar]
  11. Rueckert R. R. 1976; On the structure and morphogenesis of picomaviruses. In Comprehensive Virology vol 6: pp 131–213 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Academic Press;
    [Google Scholar]
  12. Yoon J. W., Mcclintock P. R., Onodera T., Notkins A. L. 1980; Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis virus. Journal of Experimental Medicine 152:878–892
    [Google Scholar]
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