1887

Journal of General Virology header logo

Volume 64, Issue 4

Modification of Membrane Permeability in Poliovirus-infected HeLa Cells: Effect of Guanidine Free

Abstract

SUMMARY

A drastic modification in permeability to monovalent ions occurred in HeLa cells infected with poliovirus, starting in the period from the third to the fourth h post-infection. The bulk of poliovirus protein synthesis took place from the third to the sixth h in cells in which the concentration of monovalent ions in the cytoplasm had changed considerably compared to uninfected cells. Under our conditions of infection (HeLa cells grown in monolayer), poliovirus translation continued beyond the eighth h. Modification of permeability to Rb ions induced by poliovirus infection was prevented by the addition of 2 m-guanidine at the time of infection. However, when poliovirus replication was allowed to take place for 1 h before addition of guanidine, the membrane did become modified to some extent. The degree of leakiness to Rb ions increased when guanidine was added later. The blockage of membrane leakiness by early addition of guanidine was overcome by the addition of choline. The inhibition of cellular protein synthesis, which occurred early in infection or in the presence of guanidine, did not coincide with the modification of permeability to Rb ions. Viral protein synthesis was necessary in order to modify the membrane late in infection, since addition of 10 -cycloheximide during the first 2 h of infection prevented the leakiness to Rb ions observed from the fourth h after infection. Membrane potential, as measured by the lipophilic cation TPP (tetraphenylphosphonium), dropped from the fourth h post-infection. This change in the membrane was also prevented when viral gene expression was inhibited by the presence of guanidine.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): guanidine , poliovirus , shut-off and translation
© Journal of General Virology 1983
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-4-787
1983-04-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/4/JV0640040787.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-4-787&mimeType=html&fmt=ahah

References

  1. Abreu S. L., Lucas-Lenard J. 1976; Cellular protein synthesis shut-off by mengovirus: translation of nonviral and viral mRNA’s in extracts from uninfected Ehrlich ascites tumour cells. Journal of Virology 18:182–194
     [Google Scholar]
  2. Ackermann W. W., Loh P. C., Pavne F. E. 1959; Studies of the biosynthesis of protein and ribonucleic acid in HeLa cells infected with poliovirus. Virology 7:170–183
     [Google Scholar]
  3. Bablanian R. 1975; Structural and functional alterations in cultured cells infected with cytocidal viruses. Progress in Medical Virology 19:14–83
     [Google Scholar]
  4. Baltimore D. 1969; The replication of picornavirus. In The Biochemistry of Viruses pp 101–176 Edited by Levy H. B. New York & London: Marcel Dekker;
     [Google Scholar]
  5. Carrasco L. 1981; Modification of membrane permeability induced by animal viruses early in infection. Virology 113:623–629
     [Google Scholar]
  6. Carrasco L., Esteban M. 1981; Modification of membrane permeability in vaccinia virus-infected cells. Virology 117:62–69
     [Google Scholar]
  7. Carrasco L., Smith A. E. 1976; Sodium ions and the shut-off of host cell protein synthesis by picomaviruses. Nature, London 264:807–809
     [Google Scholar]
  8. Carrasco L., Smith A. E. 1980; Molecular biology of animal virus infection. Pharmacology and Therapeutics 9:311–355
     [Google Scholar]
  9. Carrasco L., Harvey R., Blanchard C., Smith A. E. 1979; Regulation of translation of eukaryotic virus mRNAs. In Modem Trends in Human Leukemia vol 3: pp 189–192 Edited by Neth R., Hofschneider P., Mannweiler K. Wien & New York: Springer-Verlag;
     [Google Scholar]
  10. Contreras A., Carrasco L. 1979; Selective inhibition of protein synthesis in virus-infected mammalian cells. Journal of Virology 29:114–122
     [Google Scholar]
  11. Crowther D., Melnick J. L. 1961; Studies of the inhibitory action of guanidine on poliovirus multiplication of cell culture. Virology 15:65–74
     [Google Scholar]
  12. Egberts E., Hackett P. B., Traub P. 1977; Alteration of the intracellular energetic and ionic conditions by mengovirus infection of Ehrlich ascites tumour cells and its influence on protein synthesis in the midphase of infection. Journal of Virology 22:591–597
     [Google Scholar]
  13. Farham A. E., Epstein W. 1963; The influence of EMC virus infection on potassium transport in L-cells. Virology 21:436–447
     [Google Scholar]
  14. Helentjaris T., Ehrenfeld E. 1978; Control of protein synthesis in extracts from poliovirus-infected cells. I. mRNA discrimination by crude initiation factors. Journal of Virology 26:510–521
     [Google Scholar]
  15. Jen G., Detjen B. M., Thach R. E. 1980; Shut-off of HeLa cell protein synthesis by encephalomyocarditis virus and poliovirus: a comparative study. Journal of Virology 35:150–156
     [Google Scholar]
  16. Kohn S. 1979; Early interactions of viruses with cellular membranes. Advances in Virus Research 24:223–276
     [Google Scholar]
  17. Lacal J. C., Carrasco L. 1982; Relationship between membrane integrity and the inhibition of host translation in virus-infected mammalian cells. Comparative studies between encephalomyocarditis virus and poliovirus. European Journal of Biochemistry 127:359–366
     [Google Scholar]
  18. Lacal J. C., Vazquez D., Fernandez-Sousa J. M., Carrasco L. 1980; Antibiotics that specifically block translation in virus-infected cells. Journal of Antibiotics 33:441–447
     [Google Scholar]
  19. Levanon A., Koch A., Inbar M. 1977; Increase in lipid fluidity of cellular membranes induced by adsorption of RNA and DNA virions. Journal of Virology 22:353–360
     [Google Scholar]
  20. Lichtstein D., Kaback H. R., Blume A. J. 1979; Use of a lipophylic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspension. Proceedings of the National Academy of Sciences, U. S. A 76:650–654
     [Google Scholar]
  21. Loddo B., Ferrari W., Spanedda A., Brotzu G. 1962; ‘In vitro’ inhibition of infectivity of poliovirus by guanidine. Nature, London 193:97–98
     [Google Scholar]
  22. Mosser A. G., Calguiri L. A., Tamm I. 1971; Blocking of guanidine action on poliovirus multiplication. Virology 45:653–663
     [Google Scholar]
  23. Nair C. N. 1981; Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells. Journal of Virology 37:268–273
     [Google Scholar]
  24. Nair C. N., Stowers J. W., Singfield B. 1979; Guanidine-sensitive Na+ accumulation by poliovirus-infected HeLa cells. Journal of Virology 31:184–189
     [Google Scholar]
  25. Nuss D. L., Koch G. 1976; Differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. Journal of Virology 17:283–286
     [Google Scholar]
  26. Nuss D. L., Opperman H., Koch G. 1975; Selective blockage of initiation of host protein synthesis in RNA virus-infected cells. Proceedings of the National Academy of Sciences, U. S. A 72:1258–1262
     [Google Scholar]
  27. Pasternak C. A., Micklem K. J. 1981; Virally induced alterations in cellular permeability: a basis of cellular and physiological damage?. Bioscience Reports 1:431–448
     [Google Scholar]
  28. Rightsel W. A., Dice J. R., McAlpine R. J., Timm E. A., McLean I. W., Dixon G. I., Schabel F. M. 1961; Antiviral effect of guanidine. Science 134:558–559
     [Google Scholar]
  29. Rose J. K., Trachsel H., Leong K., Baltimore D. 1978; Inhibition of translation by poliovirus: inactivation of a specific initiation factor. Proceedings of the National Academy of Sciences, U. S. A. 75:2732–2736
     [Google Scholar]
  30. Saborio J. L., Pong S. S., Koch G. 1974; Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. Journal of Molecular Biology 85:195–211
     [Google Scholar]
  31. Salzman N. P., Lockart R. Z., Sebring E. D. 1959; Alterations in HeLa cell metabolism resulting from poliovirus infection. Virology 9:244–259
     [Google Scholar]
  32. Trachsel H., Sonenberg N., Shatkin A. J., Rose J. K., Leong K., Bergmann J. E., Gordon J., Baltimore D. 1980; Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences, U. S. A 77:770–774
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-4-787
Loading
Modification of Membrane Permeability in Poliovirus-infected HeLa Cells: Effect of Guanidine
J. Gen. Virol. 64, 787 (1983); https://doi.org/10.1099/0022-1317-64-4-787
/content/journal/jgv/10.1099/0022-1317-64-4-787
/content/journal/jgv/10.1099/0022-1317-64-4-787
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error