1887

Abstract

Summary

A hybrid murine myeloma (2-13F5) secreting a monoclonal antibody that specifically bound and neutralized murine interferon β has been isolated. Affinity chromatography with immobilized 2-13F5 monoclonal antibody of murine IFN (containing both IFN-α and IFN-β) separated an IFN-β preparation that demonstrated one band with Coomassie Brilliant Blue staining of SDS-PAGE at a molecular weight of 35000 and a specific activity of 4.3 × 10 units/mg of protein. This monoclonal antibody neither bound nor neutralized murine IFN-α. In addition, it bound only 87% of the interferon demonstrating a molecular weight of 35K. Possible explanations of this partial binding are discussed.

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/content/journal/jgv/10.1099/0022-1317-64-3-727
1983-03-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-3-727
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