1887

Abstract

SUMMARY

Ribonucleotide reductase, an enzyme found in all prokaryotic and eukaryotic cells that synthesize DNA, is induced by herpes simplex virus (HSV). In this study the effect of anti-HSV antiserum on the induced ribonucleotide reductase has been examined and the ability of different temperature-sensitive () mutants of HSV-1 to induce the enzyme has been investigated. The HSV-1-induced ribonucleotide reductase was inhibited by antiserum raised against infected cell lysates but not by preimmune serum. The wild-type ( ) virus induced similar levels of ribonucleotide reductase at 31 °C and 38.5 °C (the permissive and non-permissive temperatures respectively for the mutants). All mutants induced approximately wild-type levels of the enzyme at 31 °C. At 38.5 °C, two of the four mutants studied also induced wild-type levels of enzyme but G failed to induce any activity while K induced variable but low levels. The enzyme activity induced by G at 31 °C was thermolabile both and . These results provide the first strong evidence that the induced ribonucleotide reductase activity is at least partially virus-coded.

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/content/journal/jgv/10.1099/0022-1317-64-3-513
1983-03-01
2022-01-20
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