Ribonucleotide reductase, an enzyme found in all prokaryotic and eukaryotic cells that synthesize DNA, is induced by herpes simplex virus (HSV). In this study the effect of anti-HSV antiserum on the induced ribonucleotide reductase has been examined and the ability of different temperature-sensitive () mutants of HSV-1 to induce the enzyme has been investigated. The HSV-1-induced ribonucleotide reductase was inhibited by antiserum raised against infected cell lysates but not by preimmune serum. The wild-type ( ) virus induced similar levels of ribonucleotide reductase at 31 °C and 38.5 °C (the permissive and non-permissive temperatures respectively for the mutants). All mutants induced approximately wild-type levels of the enzyme at 31 °C. At 38.5 °C, two of the four mutants studied also induced wild-type levels of enzyme but G failed to induce any activity while K induced variable but low levels. The enzyme activity induced by G at 31 °C was thermolabile both and . These results provide the first strong evidence that the induced ribonucleotide reductase activity is at least partially virus-coded.


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