1887

Abstract

SUMMARY

Radioactive choline was incorporated by HeLa cells into a characteristic distribution of choline-associated lipid macromolecules when cytoplasmic extracts are centrifuged in discontinuous sucrose gradients. The proportional distributions of radioactivity among three peaks in the gradients were altered by infecting the HeLa cells with rhinovirus type 2 and 14, or with poliovirus. Poliovirus infection induced similar distributions of radioactivity in both LLCMK and HeLa cells with the greatest radioactivity increase in peaks nearest the tops of the gradients. When the LLCMK cells were infected with echovirus 12, the major increase in choline-associated radioactivity was in a peak near the middle of the gradients, in a pattern of distribution that was more similar to that of rhinovirus-infected HeLa cells than to poliovirus-infected LLCMK cells. Radioactive glucosamine-labelled, cytoplasmic macromolecules were distributed differently from those labelled with choline in both infected and uninfected cultures, and infection by all four virus strains sharply decreased glucosamine-associated radioactivity in the gradients, suggesting that the synthesis of glucosamine- and choline-containing structures are under different cytoplasmic controls. When these results are taken together with the findings of clearly different patterns of distribution of choline-associated radioactivity in gradients prepared from uninfected HeLa and LLCMK cells labelled at 37 °C, and the closer similarity between them when labelled at 34 °C, it appears that choline-containing lipids can have specific temperature-regulated processes in different cell types, and that different viruses may have common and selectively specific modifying effects on the synthesis of cytoplasmic lipid macromolecules.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-2-491
1983-02-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/2/JV0640020491.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-2-491&mimeType=html&fmt=ahah

References

  1. Amako K., Dales S. 1967; Cytopathology of Mengovirus infection. II. Proliferation of membranous cistemae. Virology 32:201–215
    [Google Scholar]
  2. Bosmann B., Hagopian A., Eylar E. H. 1968; Cellular membranes: the isolation and characterization of the plasma and smooth membranes of HeLa cells. Archives of Biochemistry and Biophysics 128:51–69
    [Google Scholar]
  3. Caliguiri L. A., Tamm I. 1969; Membranous structures associated with translation and transcription of poliovirus RNA. Science 166:855–886
    [Google Scholar]
  4. Caliguiri L. A., Tamm I. 1970; The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 42:100–111
    [Google Scholar]
  5. Cornatzer W. E., Sandstrom W., Fischer R. G. 1961; The effect of poliomyelitis virus type I (Mahoney strain) on the phospholipid metabolism of the HeLa cell. Biochimica et biophysica acta 49:414–415
    [Google Scholar]
  6. Dales S., Eggers H. J., Tamm I., Palade G. E. 1965; Electron microscopic study of the formation of poliovirus. Virology 26:379–389
    [Google Scholar]
  7. Darnell J. R. J. 1968; Consideration of virus-controlled functions. In The Molecular Biology of Viruses pp 375–401 Edited by Colter J. S., Paranchych W. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis virus. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  9. Freifelder D. 1976 Physical Biochemistry: Applications to Biochemistry and Molecular Biology p 384 San Francisco: W. H. Freeman and Company;
    [Google Scholar]
  10. Halperen S., Stone H. O., Korant B. D. 1973; Isolation of glucosamine from the capsids of a picomavirus. Journal of General Virology 20:267–276
    [Google Scholar]
  11. Kawana R., Matsumoto I. 1971; Electron microscopic study of rhinovirus replication in human fetal lung cells. Japanese Journal of Microbiology 15:207–217
    [Google Scholar]
  12. Lodish H. F., Braell W. A., Schwartz A. L., Strous-Ger J. A. M., Zilberstein A. 1981; Synthesis and assembly of membrane and organelle proteins. In Membrane Research, Classic Origins and Current Concepts pp 264–280 Edited by Muggleton-Harris A. L. New York: Academic Press;
    [Google Scholar]
  13. Mosser A. G., Caliguiri L. A., Scheid A. S., Tamm L. 1972a; Chemical and enzymatic characteristics of cytoplasmic membranes of poliovirus-infected HeLa cells. Virology 47:30–38
    [Google Scholar]
  14. Mosser A. G., Caliguiri L. A., Tamm I. 1972b; Incorporation of lipid precursors into cytoplasmic membranes of poliovirus-infected HeLa cells. Virology 47:39–47
    [Google Scholar]
  15. Penman S. 1965; Stimulation of the incorporation of choline in poliovirus infected cells. Virology 25:148–152
    [Google Scholar]
  16. Putnak R. J., Phillips B. A. 1981; Picomaviral structure and assembly. Microbiological Reviews 45:287–315
    [Google Scholar]
  17. Skinner M. S., Halperen S., Harkin J. C. 1968; Cytoplasmic membrane-bound vesicles in echovirus 12-infected cells. Virology 36:241–253
    [Google Scholar]
  18. Yin F. H. 1977; Involvement of viral procapsid in the RNA synthesis and maturation of poliovirus. Virology 82:229–307
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-2-491
Loading
/content/journal/jgv/10.1099/0022-1317-64-2-491
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error