1887

Abstract

SUMMARY

We established three hybridomas (designated 2A12, 4C4 and 6H8) secreting monoclonal antibodies which react with N and H(heated) antigenic particles of poliovirus type 1 (Mahoney strain). One of these monoclonal antibodies, 4C4, had virus-neutralizing activity, while the other two were non-neutralizing. A solid-phase radioimmunoassay and a neutralization test indicated that the specificity of the neutralizing monoclonal antibody 4C4 was the same as that of the monospecific antibody HN31, previously prepared by us by absorbing poliovirus antiserum with an equine serum inhibitor-resistant mutant (M-HN31) of poliovirus. Furthermore, by means of immune electron microscopy, it was revealed that the combining site of the 4C4 neutralizing monoclonal antibody is on or around vertices of the N and H antigenic particles, while the other two monoclonal antibodies are directed to the entire surface of the antigens.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-12-2803
1983-12-01
2024-06-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/12/JV0640122803.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-12-2803&mimeType=html&fmt=ahah

References

  1. Blondel B., Akacem O., Crainic R., Couilin P., Horodniceanu F. 1983; Detection by monoclonal antibodies of an antigenic determinant critical for poliovirus neutralization present on VP1 and on heat-inactivated virions. Virology 126:707–710
    [Google Scholar]
  2. Boeyé A., Van Elsen A. 1967; Alkaline disruption of poliovirus: kinetics and purification of RNA-free particles. Virology 3:335–343
    [Google Scholar]
  3. Breindl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  4. Brioen P., Sijens R. J., Vrijsen R., Rombaut B., Thomas A. A. M., Jackers A., Boeyé A. 1982; Hybridoma antibodies to poliovirus N and H antigen. Archives of Virology 74:325–330
    [Google Scholar]
  5. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. 1982; Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. Journal of Virology 43:997–1005
    [Google Scholar]
  6. Hinuma Y., Katagiri S., Aikawa S. 1970; Immune responses to H particles of poliovirus. Virology 40:773–776
    [Google Scholar]
  7. Hummeler K., Tumilowicz J. J. 1960; Studies on the complement-fixing antigens of poliomyelitis. II.Preparation of type-specific anti-N and anti-H indicator sera. Journal of Immunology 84:630–634
    [Google Scholar]
  8. Hummeler K., Anderson T. F., Brown R. A. 1962; Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology 16:84–90
    [Google Scholar]
  9. Icenogle J., Gilbert S. F., Grieves J., Anderegg J., Rueckert R. 1981; A neutralizing monoclonal antibody against poliovirus and its reaction with related antigens. Virology 115:211–215
    [Google Scholar]
  10. Katagiri S., Hinuma Y., Ishida N. 1967; Biophysical properties of poliovirus particles irradiated with ultra-violet light. Virology 32:337–343
    [Google Scholar]
  11. Katagiri S., Aikawa S., Hinuma Y. 1971; Stepwise degradation of poliovirus capsid by alkaline treatment. Journal of General Virology 13:101–109
    [Google Scholar]
  12. Le Bouvier G. L. 1959; Poliovirus D and C antigens. Their differentiation and measurement by precipitation in agar. British Journal of Experimental Pathology 40:452–463
    [Google Scholar]
  13. Mayer M. M., Rapp H. J., Roizman B., Klein S. W., Cowan K. M., Lukens D., Schwerdt C. E., Schaffer F. L., Charney J. 1957; The purification of poliomyelitis virus as studied by complement fixation. Journal of Immunology 78:435–455
    [Google Scholar]
  14. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. 1979; The isolation of hybrid cell lines producing monoclonal antibodies against the pl5(E) protein of ecotropic murine leukemia viruses. Virology 93:111–126
    [Google Scholar]
  15. Roizman B., Mayer M. M., Roane P. R. JR 1959; Immunochemical studies of poliovirus. IV. Alteration of immunologic specificity of purified poliomyelitis virus by heat and ultraviolet. Journal of Immunology 82:19–25
    [Google Scholar]
  16. Taniguchi K., Urasawa S., Urasawa T. 1981; Further studies of 35 to 40 nm virus-like particles associated with outbreaks of acute gastroenteritis. Journal of Medical Microbiology 14:107–118
    [Google Scholar]
  17. Taniguchi K., Urasawa S., Urasawa T. 1983; Electron microscopic examination of the reactivity of three kinds of neutralizing antibodies with H particles of poliovirus. Microbiology and Immunology 27:645–648
    [Google Scholar]
  18. Thorpe R., Minor P. D., Mackay A., Schild G. C., Spitz M. 1982; Immunochemical studies of polioviruses: identification of immunoreactive virus capsid polypeptides. Journal of General Virology 63:487–492
    [Google Scholar]
  19. Urasawa S., Urasawa T., Kanamitsu M. 1971; Radioimmunoelectrophoretic identification of poliovirus inhibitors and their characteristic mode of action. Archiv für die gesamte Virusforschung 33:113–125
    [Google Scholar]
  20. Urasawa S., Urasawa T., Kanamitsu M. 1974; Analysis of specificity of antibodies contained in antiserum against poliovirus with mutants resistant to inhibitors in equine serum. Journal of Immunology 113:537–542
    [Google Scholar]
  21. Urasawa S., Urasawa T., Kanamitsu M. 1976; Further studies of specificity of antibodies contained in antiserum against poliovirus. Japanese Journal of Microbiology 20:11–16
    [Google Scholar]
  22. Urasawa T., Urasawa S., Taniguchi K. 1979; Reactivity of neutralizing antibodies with different specificities to H particles of poliovirus. Microbiology and Immunology 23:651–657
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-12-2803
Loading
/content/journal/jgv/10.1099/0022-1317-64-12-2803
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error