1887

Abstract

SUMMARY

Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include , a plant not known to be susceptible. With this technique >90% of turnip protoplasts were infected, each producing an average of 10 to 10 viral genome equivalents. Filter hybridization and fluorescent antibody staining showed that virus synthesis in turnip protoplasts reaches a maximum 65 to 96 h post-inoculation. In protoplasts from some other species the time course was different; viral DNA synthesis in rape reached a maximum 24 to 48 h post-inoculation, approximately 48 h before the maximum accumulation of viral antigen.

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/content/journal/jgv/10.1099/0022-1317-64-12-2655
1983-12-01
2024-04-26
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References

  1. Furusawa I., Yamaoka N., Okuno T., Yamamoto M., Kohno M., Kunoh H. 1980; Infection of turnip protoplasts with cauliflower mosaic virus. Journal of General Virology 48:431–435
    [Google Scholar]
  2. Hohn T., Richards K., Lebeurier G. 1981; Cauliflower mosaic virus on its way to becoming a useful plant vector. Current Topics in Microbiology and Immunology 96:194–236
    [Google Scholar]
  3. Howell S. H., Hull R. 1978; Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts. Virology 86:468–481
    [Google Scholar]
  4. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
    [Google Scholar]
  5. Kao K. N., Michayluk M. R. 1975; Nutritional requirements for growth of Vicia hajastuna cells and protoplasts at a very low population density in liquid media. Planta 126:105–110
    [Google Scholar]
  6. Kartha K. K., Michayluk M. R., Kao K. N., Gamborg O. L., Constabel F. 1974; Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L. cv. Zephyr). Plant Science Letters 3:265–271
    [Google Scholar]
  7. Maule A. J., Boulton M. I., Edmunds C., Wood K. R. 1980a; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  8. Maule A. J., Boulton M. I., Wood K. R. 1980b; An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathologische Zeitschrift 97:118–126
    [Google Scholar]
  9. Maule A. J., Hull R., Donson J. 1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6:215–224
    [Google Scholar]
  10. Rottier P. J. M., Rezelman G., VAN Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
    [Google Scholar]
  11. Takebe I., Otsuki Y., Aoki S. 1968; Isolation of tobacco mesophyll cells in intact and active state. Plant and Cell Physiology 9:115–124
    [Google Scholar]
  12. Ulrich T. H., Chowdhury J. B., Widholm J. M. 1980; Callus and root formation from mesophyll protoplasts of Brassica rapa. Plant Science Letters 19:347–354
    [Google Scholar]
  13. Vatsya B., Bhaskaran S. 1982; Plant regeneration from cotyledonary protoplasts of cauliflower (Brassica oleracea L. var. botrytis L.). Protoplasma 113:161–163
    [Google Scholar]
  14. Yamaoka N., Furusawa I. I., Yamamoto M. 1982a; Infection of turnip protoplasts with cauliflower mosaic virus DNA. Virology 122:503–505
    [Google Scholar]
  15. Yamaoka N., Morita T., Furusawa I., Yamamoto M. 1982b; Effect of temperature on the multiplication of cauliflower mosaic virus. Journal of General Virology 61:283–287
    [Google Scholar]
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