1887

Abstract

SUMMARY

The organization of vimentin filaments, keratin filaments, microtubules and microfilaments was compared in canine distemper virus (CDV)-infected and uninfected cells by indirect immunofluorescence. Infection of tissue culture cells with CDV caused a total reorganization of all the cytoskeletal structures with the most notable changes in the microtubules and intermediate filaments. During virus infection two different patterns of staining were observed for both the intermediate filaments and microtubules, suggesting a step-by-step reorganization of the structures. While the two types of intermediate filaments (vimentin and keratin) had quite different staining patterns, the vimentin (but not keratin) filaments had a distribution pattern similar to the microtubules in both infected and uninfected cells. These results suggest that microtubules and vimentin (but not keratin) filaments may have a close association in CDV-infected cells.

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1983-11-01
2024-05-04
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References

  1. Bourguignon L. Y. W., Rozek R. J. 1978; Differential transmembrane interactions in normal and malignant fibroblasts. Journal of Cell Biology 79:51a
    [Google Scholar]
  2. Bourguignon L. Y. W., Rozek R. J. 1980; Capping of concanavalin A receptors and their association with microfilaments in monolayer grown human fibroblastoid cells. Cell and Tissue Research 205:877–884
    [Google Scholar]
  3. Bourguignon L. Y. W., Singer S. J. 1977; Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands. Proceedings of the National Academy of Sciences, U. S. A 74:5031–5035
    [Google Scholar]
  4. Bourguignon L. Y. W., Tokuyasu K. T., Singer S. J. 1978; The capping of lymphocytes and other cells, studied by an improved method for immuno-fluorescence staining of frozen sections. Journal of Cell Physiology 95:239–258
    [Google Scholar]
  5. Bourguignon L. Y. W., Nagpal M. L., Hsing Y. C. 1981; Phosphorylation of myosin chain during capping of mouse T-lymphoma cells. Journal of Cell Biology 91:889–894
    [Google Scholar]
  6. Bussell R. H., Karzon D. T. 1962; Canine distemper virus in chick embryo cell culture. Virology 18:589–600
    [Google Scholar]
  7. Bussell R. H., Karzon D. T. 1965a; Canine distemper virus in ferret, dog and bovine kidney cell cultures. Archiv fur die gesamte Virusforschung 17:163–182
    [Google Scholar]
  8. Bussell R. H., Karzon D. T. 1965b; Canine distemper virus in primary and continuous cell lines of human and monkey origin. Archiv für die gesamte Virusforschung 17:183–202
    [Google Scholar]
  9. Clark M., Spudich J. A. 1977; Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annual Review of Biochemistry 46:797–822
    [Google Scholar]
  10. Eckert B. S., Daley R. A. 1981; Response of cultured mammalian cells (PtKl) to microinjection of antibody specific for keratin. Biologie Cellulaire 41:227–230
    [Google Scholar]
  11. Eckert B. S., Daley R. A., Parysek L. M. 1982; Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center. Journal of Cell Biology 92:575–578
    [Google Scholar]
  12. Fagraeus A., Tyrrell D. L. J., Norberg R., Norrby E. 1978; Actin filaments in paramyxovirus-infected human fibroblasts studied by indirect immunofluorescence. Archives of Virology 57:291–296
    [Google Scholar]
  13. Franke W. W., Schmid E., Osborn M., Weber K. 1978; Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proceedings of the National Academy of Sciences, U. S. A 75:5034–5038
    [Google Scholar]
  14. Franke W. W., Schmid E., Winter S., Osborn M., Weber K. 1979; Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Experimental Cell Research 123:25–46
    [Google Scholar]
  15. Geiger B., Singer S. J. 1980; Association of microtubules and intermediate filaments in chicken gizzard cells as detected by double immunofluorescence. Proceedings of the National Academy of Sciences, U. S. A 77:4769–4773
    [Google Scholar]
  16. Goldman D., Milsted A., Schloss J. A., Starger J., Yerna M. J. 1979; Cytoplasmic fibers in mammalian cells: cytoskeletal and contractile elements. Annual Review of Physiology 41:703–722
    [Google Scholar]
  17. Heeg U., Hasse W., Brauer D., Falke D. 1981; Microtubules and microfilaments in HSV-infected rabbit-kidney cells. Archives of Virology 70:233–246
    [Google Scholar]
  18. Howard J. 1981; A possible role for intermediate filaments in the cytopathic effects of canine distemper virus infected cells. Anatomical Record 199: 120A (abstract)
    [Google Scholar]
  19. Hynes R. O., Destree A. T. 1978; 10 nm filaments in normal and transformed cells. Cell 13:151–163
    [Google Scholar]
  20. Meyer R. K., Burger M. M., Tschannen R., Schafer R. 1981; Actin filament bundles in vaccinia virus infected fibroblasts. Archives of Virology 67:11–18
    [Google Scholar]
  21. Pollack R., Osborn M., Weber K. 1975; Patterns of organization of actin and myosin in normal and transformed cultured cells. Proceedings of the National Academy of Sciences, U. S. A 72:994–998
    [Google Scholar]
  22. Rima B. K., Martin S. J. 1976; Persistent infections of tissue culture cells by RNA viruses. Medical Microbiology and Immunology 162:89–118
    [Google Scholar]
  23. Rubin R. W., Warren R. H., Lukeman D. S., Clements E. 1978; Actin content and organization in normal and transformed cells in culture. Journal of Cell Biology 78:28–35
    [Google Scholar]
  24. Rubin R. W., Howard J., Leonardi C. 1979; A biochemical and ultrastructural comparison of Triton X-100 models of normal and transformed cells. Tissue and Cell 11:413–423
    [Google Scholar]
  25. Rutter G., Mannweiler K. 1977; Alteration of actin-containing structures in MKH21 cells infected with Newcastle disease virus and vesicular stomatitis virus. Journal of General Virology 37:233–242
    [Google Scholar]
  26. Sharpe A. H., Chen L. B., Fields B. N. 1982; The interaction of mammalian reoviruses with the cytoskeleton of monkey kidney CV-1 cells. Virology 120:399–411
    [Google Scholar]
  27. Sheetz M. P. K., Painter R. G., Singer S. J. 1976; Relationships of the spectrin complex of human erythrocyte membranes to the actomyosin of muscle cells. Biochemistry 15:4486–4492
    [Google Scholar]
  28. Stephens R. E., Edds K. T. 1976; Microtubules: structure, chemistry, and function. Physiological Reviews 56:709–773
    [Google Scholar]
  29. Ter Meulen V., Martin S. J. 1976; Genesis and maintenance of a persistent infection by canine distemper virus. Journal of General Virology 32:431–440
    [Google Scholar]
  30. Wang E. R., Cross K., Choppin P. W. 1979; Involvement of microtubules and 10 nm filaments in the movement and positioning of nuclei in syncytia. Journal of Cell Biology 83:320–337
    [Google Scholar]
  31. Weber K., Grolschel-Stewart W. 1974; Antibody to myosin: the specific visualization of myosin containing filaments in nonmuscle cells. Proceedings of the National Academy of Sciences, U. S. A 71:4561–4564
    [Google Scholar]
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