1887

Abstract

SUMMARY

Inhibitors of bacterial DNA gyrase and eukaryotic DNA topoisomerase (novobiocin and nalidixic acid) were investigated with respect to their effect on the activity of RNA-dependent DNA polymerases from murine and avian retroviruses. Purified RNA-dependent DNA polymerase from AKR virus was inhibited more than 90% by 0.3 mg/ml and almost completely by 1 mg/ml of the drugs when poly(A).oligo(dT) was used as a template-primer. In contrast to the enzyme from AKR virus, purified enzyme from avian myeloblastosis virus was less sensitive, i.e. nearly 50% activity remained even in the presence of 1 mg/ml of the drugs with the same template-primer. RNA-dependent DNA polymerase activity in AKR virus particles was inhibited, but was resistant to low concentrations of the drugs. The inhibition was not due to specific interaction between drugs and the template-primer or labelled precursor, since RNA-dependent DNA polymerase was inhibited by the drugs with activated calf thymus DNA or poly(C).oligo(dG) as the template. Endogenous DNA synthesis by AKR virus particles was inhibited by novobiocin to the same extent.

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/content/journal/jgv/10.1099/0022-1317-64-10-2329
1983-10-01
2021-10-25
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References

  1. Bronson D. L., Elliott A. Y., Ritzi D. 1975; Concentration of Rous sarcoma virus from tissue culture fluidswith polyethylene glycol. Applied Microbiology 30:464–471
    [Google Scholar]
  2. Cozzarelli N. R. 1977; The mechanism of action of inhibitors of DNA synthesis. Annual Review of Biochemistry 46:641–668
    [Google Scholar]
  3. Cozzarelli N. R. 1980; DNA gyrase and supercoiling of DNA. Science 207:953–960
    [Google Scholar]
  4. Edenberg H. L. 1980; Novobiocin inhibition of simian virus 40 DNA replication. Nature, London 286:529–531
    [Google Scholar]
  5. Gellert M. 1981; DNA topoisomerase. Annual Review of Biochemistry 59:879–910
    [Google Scholar]
  6. Gellert M., O’Dea M. H., Itoh T., Tomizawa J. I. 1976; Novobiocin and coumermycin inhibit DNAsupercoiling catalyzed by DNA gyrase. Proceedings of the National Academy of Sciences, U. S. A 73:4474–4478
    [Google Scholar]
  7. Gellert M., Mizuuchi K., O’Dea M. H., Itoh T., Tomizawa J. I. 1977; Nalidixic acid resistance: a secondgenetic character involved in DNA gyrase activity. Proceedings of the National Academy of Sciences, U. S. A 74:4772–4776
    [Google Scholar]
  8. Hagino-Yamagishi K., Kano K., Mano Y. 1981; Effect of aphidicolin on retrovirus DNA synthesis in vitro. Biochemical and Biophysical Research Communications 102:1372–1378
    [Google Scholar]
  9. Hsieh T., Brutlag D. 1980; ATP-dependent DNA topoisomerase from D. melanogaster reversibly catenatesduplex DNA rings. Cell 21:115–125
    [Google Scholar]
  10. Lavin M. F. 1981; Effect of novobiocin on DNA synthesis and structure in human lymphoblastoid cells. Biochemical and Biophysical Research Communications 100:328–335
    [Google Scholar]
  11. Mattern N. R., Painter R. B. 1979; Dependence of mammalian DNA synthesis on DNA supercoiling. II.Effect of novobiocin and DNA synthesis in Chinese hamster ovary cells. Biochimica et biophysica acta 563:306–312
    [Google Scholar]
  12. Mattern N. R., Scudiero D. A. 1981; Dependence on mammalian DNA synthesis on DNA supercoiling. III.Characterization of the inhibition of replicative and repair-type DNA synthesis by novobiocin and nalidixicacid. Biochimica et biophysica acta 653:248–258
    [Google Scholar]
  13. Mattern N. R., Paone R. F., Day R. S. III 1982; Eukaryotic DNA repair is blocked at different steps byinhibitors of DNA topoisomerases and of DNA polymerase α and β. Biochimica et biophysica acta 697:6–13
    [Google Scholar]
  14. Staudenbauer W. L. 1975; Novobiocin - a specific inhibitor of semiconservative DNA replication inpermeabilizedEscherichia coli cells. Journal of Molecular Biology 96:201–205
    [Google Scholar]
  15. Verma I. M. 1977; The reverse transcriptase. Biochimica et biophysica acta 473:1–38
    [Google Scholar]
  16. Verma I. M. 1981; Reverse transcriptase. In The Enzymes vol 14 pp 87–103 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
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