1887

Abstract

Summary

DNA polymerase activity present in multiple nucleocapsid nuclear polyhedrosis virus-infected cells has been analysed by chromatography on DNA-cellulose and phosphocellulose columns. In infected cells a new fraction of activity was found to bind to the columns more strongly that did polymerase activity in uninfected cells. The infected-cell-specific DNA polymerase was purified by a combination of DNA-cellulose chromatography of crude extracts and phospho-cellulose chromatography of the semi-purified activity. The final product contained a single polypeptide of molecular weight 126 000 which was not found in uninfected cells. The purified enzyme was inhibited by aphidicolin and []-5-(2-bromovinyl)-2′-deoxyuridine triphosphate, but not by bromovinyldeoxyuridine. The enzyme was shown to be an early enzyme, probably a delayed early protein, since it was present in cells inhibited by aphidicolin which were locked into the synthesis of early proteins by the drug.

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/content/journal/jgv/10.1099/0022-1317-64-10-2229
1983-10-01
2019-11-19
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-10-2229
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