1887

Abstract

SUMMARY

Mixed infection of MDCK cells with influenza A and influenza B viruses leads to a reduction in the rate of synthesis of haemagglutinin (HA) and nucleoprotein (NP) as compared to their rate of synthesis in cells separately infected with these viruses. The reduction is much stronger for influenza A virus proteins. The synthesis of the nonstructural NS1 protein of both viruses is relatively resistant to the heterotypic interference. The synthesis of virus-specific mRNAs exhibits the same pattern: the formation of the transcripts of HA and NP genes is much more drastically reduced than the synthesis of NS gene transcripts. The effect is strongly dependent on the multiplicity of infection and on the ratio of influenza A and B viruses in the inoculum. Primary transcription in the presence of cycloheximide is almost unchanged in doubly infected cells as compared to single infection, and no indication of differential inhibition has been observed. The results are discussed in connection with the mechanism of heterotypic interference and the regulation of influenza virus protein synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-10-2139
1983-10-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/10/JV0640102139.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-10-2139&mimeType=html&fmt=ahah

References

  1. Appleyard G., Maber H. B. 1974; Plaque formation by influenza viruses in the presence of trypsin. Journal of General Virology 25:351–357
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Bratt M. A., Robinson W. S. 1967; Ribonucleic acid synthesis in cells infected with Newcastle disease virus. Journal of Molecular Biology 23:1–21
    [Google Scholar]
  4. Franklin R. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences, U. S. A 63:1504–1511
    [Google Scholar]
  5. Gotlieb T., Hirst G. K. 1954; The experimental production of combination forms of virus. III. The formation of doubly antigenic particles from influenza A and B virus and study of the ability of individual particles to yield two separate strains. Journal of Experimental Medicine 99:307–320
    [Google Scholar]
  6. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. 1977; Transcription of influenza virus genome. Virology 83:337–355
    [Google Scholar]
  7. Inglis S. C., Mahy B. W. J. 1979; Polypeptides specified by the influenza virus genome. 3. Control of synthesis in infected cells. Virology 95:154–164
    [Google Scholar]
  8. Ito Y., Joklik W. K. 1972; Temperature-sensitive mutants of reovirus. I. Patterns of gene expression by mutants of groups C, D and E. Virology 50:189–201
    [Google Scholar]
  9. Kaverin N. V., Varich N. L., Smirnov Y. A. 1973; Sedimentation pattern of virus-specific RNA synthesized in Newcastle disease virus infected cells treated with amino acid analogues. Archiv für die gesamte Virusforschung 41:191–198
    [Google Scholar]
  10. Kaverin N. V., Kolomiets L. I., Rudneva I. A. 1980; Incomplete influenza virus: partial functional complementation as revealed by hemadsorbing cell count test. Journal of Virology 34:506–511
    [Google Scholar]
  11. Kolakofsky D., Boy De La Tour E., Delius H. 1974; Molecular weight determination of Sendai and Newcastle disease virus RNA. Journal of Virology 13:261–268
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Lindberg U., Persson T. 1972; Isolation of mRNA from KB cells by affinity chromatography on polyuridilic acid covalently linked to Sepharose. European Journal of Biochemistry 31:248–254
    [Google Scholar]
  14. Mikheeva A., Ghendon Y. Z. 1982; Intrinsic interference between influenza A and B viruses. Archives of Virology 73:287–294
    [Google Scholar]
  15. Noronha-blob L., Schulze I. T. 1976; Nonreciprocal and host-dependent interference between two variants of influenza virus. Virology 70:509–516
    [Google Scholar]
  16. Racaniello V. R., Palese P. 1979; Influenza B virus genome: assignment of viral polypeptides to RNA synthesis. Journal of Virology 29:361–373
    [Google Scholar]
  17. Scholtissek C., Rohde W., Harms E. 1977; Genetic relationship between an influenza A and a B virus. Journal of General Virology 37:243–247
    [Google Scholar]
  18. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 9:143–159
    [Google Scholar]
  19. Tekamp P. A., Penhoet E. E. 1980; Quantification of influenza virus messenger RNAs. Journal of General Virology 47:449–459
    [Google Scholar]
  20. Tobita P. A., Ohori K. 1979; Heterotypic interference between influenza viruses A/Aichi/2/68 and B/ Massachusetts/1/71. Acta virologica 23:263–266
    [Google Scholar]
  21. Varich N. L., Lukashevich I. S., Kaverin N. V. 1976; Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-Sepharose. Journal of Virology 18:111–116
    [Google Scholar]
  22. Varich N. L., Petrik J., Farashyan V. R., Kaverin N. V. 1981; Virus-specific and cell-specific RNA transcripts in influenza virus-infected cells: the rate of synthesis and the content in the nuclei. Archives of Virology 68:279–284
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-64-10-2139
Loading
/content/journal/jgv/10.1099/0022-1317-64-10-2139
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error