1887

Abstract

Summary

Protoplasts isolated from suspension-cultured tobacco cells were inoculated with tobacco mosaic virus (TMV) using a procedure similar to that for tobacco mesophyll protoplasts. Fifty to 70% of protoplasts were infected, as determined by staining with fluorescent TMV antibody. The formation of progeny virus particles was substantiated by electron microscopy of sectioned protoplasts, and the virus yield was assessed by assaying the infectivity of protoplast extracts. The course of TMV multiplication was studied by following the incorporation of [H]uridine into virus RNA. The results indicated that infection in this system is synchronous and that the rate of virus multiplication is comparable to that in mesophyll protoplasts. A system of undifferentiated, growing plant cells was thus established for one-step growth of TMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-2-451
1982-12-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/2/JV0630020451.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-2-451&mimeType=html&fmt=ahah

References

  1. Aoki S., Takebe I. 1975; Replication of tobacco mosaic virus RNA in tobacco mesophyll protoplasts inoculated in vitro . Virology65343–354
    [Google Scholar]
  2. Bourque D. P., Hagiladi A., Naylor A. W. 1973; A general method for extracting intact chloroplast and cytoplasmic ribosomal RNA from higher plant leaves. Biochemical and Biophysical Research Communications51993–999
    [Google Scholar]
  3. Ferguson W. J., Braunschweiger K. I., Braunschweiger W. R., Smith J. R., McCormick J. J., Wasmann C. C., Jarvis J. P., Bell D. H., Good N. E. 1980; Hydrogen ion buffers for biological research. Analytical Biochemistry104300–310
    [Google Scholar]
  4. Fukunaga Y., Nagata T., Takebe I. 1981; Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology113752–760
    [Google Scholar]
  5. Jarvis N. P., Murakishi H. H. 1980; Infection of protoplasts from soybean cell cultures with southern bean and cowpea mosaic viruses. Journal of General Virology48365–376
    [Google Scholar]
  6. Kajita S., Matsui C., Syono K., Suzuki M., Nagata T. 1980; Fine structure of fusion bodies formed between pea root nodule and tobacco mesophyll protoplasts. Zeitschrift fur Pflanzenphysiologie97233–240
    [Google Scholar]
  7. Kassanis B. 1967; Plant tissue culture. Methods in Virology1537–566
    [Google Scholar]
  8. Kato K., Matsumoto T., Koiwai A., Nishida K., Noguchi M., Takami E. 1972; Liquid suspension cultures of tobacco cells. In Fermentation Technology Today pp. 689–695 Edited by Terui G. Osaka: Society of Fermentation Technology of Japan;
    [Google Scholar]
  9. Lesney M. S., Murakishi H. H. 1981; Infection of soybean protoplasts from cell suspension culture with bean pod mottle virus. Journal of General Virology57387–395
    [Google Scholar]
  10. Loening U. E. 1968; Molecular weight of ribosomal RNA in relation to evolution. Journal of Molecular Biology38355–365
    [Google Scholar]
  11. Motoyoshi F., Oshima N. 1968; Multiplication of tobacco mosaic virus in suspension culture of tobacco cells. Japanese Journal of Microbiology12317–320
    [Google Scholar]
  12. Murakishi H. H., Hartmann J. X., Pelcher L. E., Beachy R. N. 1970; Improved inoculation of cultured plant cells resulting in high virus titer and crystal formation. Virology41365–367
    [Google Scholar]
  13. Nagata T., Takebe I. 1970; Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta92301–308
    [Google Scholar]
  14. Nagata T., Okada K., Takebe I., Matsui C. 1981; Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Molecular and General Genetics 184:161–165
    [Google Scholar]
  15. Nishimura M., Beevers H. 1978; Isolation of intact plastids from protoplasts from castor bean endosperm. Plant Physiology 62:40–43
    [Google Scholar]
  16. Otsuki Y., Takebe I., Honda T., Matsui C. 1972a; Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology49188–194
    [Google Scholar]
  17. Otsuki T., Shimomura T., Takebe I. 1972b; Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50:45–50
    [Google Scholar]
  18. Sakai F., Takebe I. 1970; RNA and protein synthesis in protoplasts isolated from tobacco leaves. Biochimica et biophysica acta224531–540
    [Google Scholar]
  19. Takebe I. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology13105–125
    [Google Scholar]
  20. Takebe I. 1977; Protoplasts in the study of plant virus replication. In Comprehensive Virology Vol. 11 pp. 237282 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  21. Takebe I. 1980; Protoplasts as a host cell system for plant viruses. In Advances in Protoplast Research pp. 431–440 Edited by Ferenczy L., Farkas G. L. Oxford: Pergamon Press;
    [Google Scholar]
  22. Takebe I., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America64843–848
    [Google Scholar]
  23. Vasil I. K., Vasil V. 1980; Isolation and culture of protoplasts. International Review of Cytology Supplement 11B 1–19
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-2-451
Loading
/content/journal/jgv/10.1099/0022-1317-63-2-451
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error