1887

Abstract

SUMMARY

Numerous discrete lesions, which we have termed blebs, appeared in the nucleus of BHK cells 10 to 15 h after infection with herpes simplex virus type 1 (-1). They were formed in the inner portion of the nuclear envelope by the apposition of two thickened lamellae overlying a vacuole. As demonstrated by electron microscopic studies, blebs were regular and associated with the peripheral lamina in the nucleus, averaging 3.5 blebs per μm. They appeared to be associated with an enrichment of the 155K major capsid protein in the nuclear membrane subfractions as compared with the protein composition of nuclei and plasma membrane fractions. We propose that blebs represent the site of assembly of capsid proteins before insertion and eventual envelopment.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-81
1982-11-01
2023-02-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010081.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-81&mimeType=html&fmt=ahah

References

  1. Atkinson M. A., Barr S., Timbury M. C. 1978; The fine structure of cells infected with temperaturesensitive mutants of herpes simplex virus type 2. Journal of General Virology 40:103–119
    [Google Scholar]
  2. Baucke R. B., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc binding glycoprotein. Journal of Virology 32:779–789
    [Google Scholar]
  3. Bernhard W. 1969; A new staining procedure for electron microscopical cytology. Journal of Ultrastructure Research 27:250–265
    [Google Scholar]
  4. Bibor-Hardy V., Simard R. 1980; Viral nucleic acid synthesis in relation to the proliferation of nuclear envelope in HSV-infected cells. Biologie Cellulaire 39:191–196
    [Google Scholar]
  5. Bibor-Hardy V., Pouchelet M., St-Pierre E., Simard R. 1982; The nuclear matrix is involved in herpes simplex virogenesis. Virology (in press)
    [Google Scholar]
  6. Bornens M. 1977; Solubilization of chromatin with heparin and the isolation of nuclear membranes. Methods in Cell Biology 15:163–175
    [Google Scholar]
  7. Darlington R. W., Moss L. H. 1969; The envelope of herpes virus. Progress in Medical Virology 11:16–45
    [Google Scholar]
  8. Harshman X., Conlin G. J. 1978; A simplified procedure for isolating plasma membranes from cultured mouse fibroblast cells : 3T3 and SV-3T3. Analytical Biochemistry 90:98–106
    [Google Scholar]
  9. Heine J. W., Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. Journal of Virology 9:431–439
    [Google Scholar]
  10. Herzberg M., Bibor-Hardy V., Simard R. 1981; Herpes specific and α-DNA polymerase in nuclear envelope of BHK infected cells. Biochemical and Biophysical Research Communications 100:644–650
    [Google Scholar]
  11. Infante A. A., Firshein W., Hobart P., Murray L. 1976; A nuclear membrane-associated DNA complex in cultured mammalian cells capable of synthesizing DNA in vitro. Biochemistry 15:4810–4817
    [Google Scholar]
  12. Kay R. R., Johnston I. R. 1977; Rapid isolation of nuclear envelope from rat liver. Methods in Cell Biology 15:277–286
    [Google Scholar]
  13. Keller J. M., Spear P. G., Roizman B. 1970; Proteins specified by herpes simplex virus. III. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins. Proceedings of the National Academy of Sciences of the United States of America 65:865–871
    [Google Scholar]
  14. Leduc E. H., Bernhard W. 1967; Recent modifications of the glycol methacrylate embedding procedure. Journal of Ultrastructure Research 19:196–199
    [Google Scholar]
  15. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpes virus-infected cells: analysis of the polypeptides induced by wild-type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  16. Morgan C., Rose H. M., Holden M., Jones E. P. 1959; Electron microscopic observations on the development of herpes simplex virus. Journal of Experimental Medicine 110:643–656
    [Google Scholar]
  17. Moyne G., Pichard E., Bernhard W. 1978; Ultracytochemistry of SA 7 infection. Journal of General Virology 40:77–92
    [Google Scholar]
  18. Nii S., Morgan C., Rose H. M. 1968; Electron microscopy of herpes simplex virus. II. Sequence of development. Journal of Virology 2:517–536
    [Google Scholar]
  19. Pardoll P. M., Vogelstein B., Coffey D. S. 1980; A fixed site of DNA replication in eucaryotic cells. Cell 19:527–536
    [Google Scholar]
  20. Roizman B., Furlong D. 1974 The replication of herpes viruses. In Comprehensive Virology vol. 3 pp. 229–403 Edited by Fraenkel-Conrat H., Wagner R. R. New York, Heidelberg Berlin: Plenum Press;
    [Google Scholar]
  21. Schaffer P. A., Brunschwig J. P., Mccombs R. M., Benyesh-Melnick M. 1974; Electron microscopic studies of temperature-sensitive mutants of herpes simplex type 1. Virology 62:444–457
    [Google Scholar]
  22. Schwartz J., Roizman B. 1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in HEp-2 cells : electron microscopy. Journal of Virology 4:879–889
    [Google Scholar]
  23. Spear P. G. 1976; Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1 infected cells. Journal of Virology 17:991–1008
    [Google Scholar]
  24. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 9:143–159
    [Google Scholar]
  25. Spear P. G., Keller J. M., Roizman B. 1970; Proteins specified by herpes simplex virus. II. Viral glycoproteins associated with cellular membranes. Journal of Virology 5:123–131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-81
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-81
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error