1887

Abstract

SUMMARY

The applicability of the standard enzyme-linked immunosorbent assay () for the identification of togavirus infections was investigated. Optimal concentration of gradient-purified antigen was 2.5 µg/well for alphaviruses or flaviviruses when coating polystyrene microtitre plates. A procedure for producing antigen in suckling mouse brain was developed. Results obtained with could be correlated with standard serology, but in general the was more sensitive. The was specific in differentiating alphavirus antigens. Flavivirus cross-reactivity was magnified by . should be useful as a rapid screening assay for non-related antigens, avoiding the extensive techniques currently used in standard serodiagnosis.

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/content/journal/jgv/10.1099/0022-1317-63-1-237
1982-11-01
2021-10-17
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