1887

Abstract

SUMMARY

The applicability of the standard enzyme-linked immunosorbent assay () for the identification of togavirus infections was investigated. Optimal concentration of gradient-purified antigen was 2.5 µg/well for alphaviruses or flaviviruses when coating polystyrene microtitre plates. A procedure for producing antigen in suckling mouse brain was developed. Results obtained with could be correlated with standard serology, but in general the was more sensitive. The was specific in differentiating alphavirus antigens. Flavivirus cross-reactivity was magnified by . should be useful as a rapid screening assay for non-related antigens, avoiding the extensive techniques currently used in standard serodiagnosis.

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1982-11-01
2024-04-26
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References

  1. Aizawa C., Hasegawa S., Chih-Yuan C., Yoshioka I. 1980; Large-scale purification of Japanese encephalitis virus from infected mouse brain for preparation of vaccine. Applied and Environmental Microbiology 39:54–57
    [Google Scholar]
  2. Casals J., Brown L. 1954; Hemagglutination with arthropod-borne viruses. Journal of Experimental Medicine 99:429–499
    [Google Scholar]
  3. Chappell W., Bucca M., White L., Gamble W. (editors) 1974 Arbovirus reagentsa. In Production Manual: Viral, Rickettsial, Chlamydial, and Mycoplasmal Reagents, 5th. edn pp. 4–6 Georgia: U.S. Department of Health, Education, and Welfare, Center for Disease Control;
    [Google Scholar]
  4. Clarke D., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  5. Dittmar D., Cleary T., Castro A. 1979; Immunoglobulin G and M-specific enzyme-linked immunosorbent assay for detection of Dengue antibodies. Journal of Clinical Microbiology 9:498–502
    [Google Scholar]
  6. Frazier C., Shope R. 1979; Detection of antibodies to alphaviruses by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 10:583–585
    [Google Scholar]
  7. Hoffmann H., Frisch-Niggemeyer W., Heinz F. 1979; Rapid diagnosis of tick-borne encephalitis by means of enzyme-linked immunosorbent assay. Journal of General Virology 42:505–511
    [Google Scholar]
  8. Monath T. 1976 Togaviruses, bunyaviruses, and Colorado tick fever virus. In Manual of Clinical Microbiology pp. 456–462 Rose N. R., Friedman H. H. Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  9. Roehrig J., Corser J., Schlesinger M. 1980; Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus. Virology 101:41–49
    [Google Scholar]
  10. Trent D., Grant J. 1980; A comparison of New World alphaviruses in the western equine encephalitis complex by immunochemical and oligonucleotide fingerprint techniques. Journal of General Virology 47:261–282
    [Google Scholar]
  11. Voller A., Bidwell D., Bartlett A. 1976 Microplate immunoassay for the immunodiagnosis of virus infections. In Handbook of Clinical Immunology pp. 506–512 Edited by Rose N. R., Friedma H. H. Washington, D.C: American Society for Microbiology;
    [Google Scholar]
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