1887

Abstract

SUMMARY

Ten new thermosensitive () mutants of alfalfa mosaic virus (AMV), a plant virus with a tripartite genome, are described. Stable mutants were induced by u.v. irradiation of purified middle (M) component. Mutants were isolated by subculturing in bean plants (instead of in the usual plant host, tobacco), in which unstable mutants apparently arise spontaneously. As well as mutants, we found a mutant which in contrast to the parent strain (AMV 425) was able to infect bean plants systemically. As expected, all stable mutations mapped on M component. Complementation analysis confirmed the presence of two complementation groups on M component. This complementation is probably intracistronic. Furthermore, we found that mutants belonging to the same complementation group often interfered with the multiplication of each other, even at the permissive temperature. Taken together, these results could suggest that the product directed by AMV RNA 2 (the RNA inside M component) has two functional domains and is active in a multimeric form.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-189
1982-11-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010189.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-189&mimeType=html&fmt=ahah

References

  1. Atabekov J. G., Morozov S. Y. 1979; Translation of plant virus messenger RNAs. Advances in Virus Research 25:1–91
    [Google Scholar]
  2. Bancroft J. B., Rees M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  3. Bol J. F., Van vloten-doting L., Jaspars E. M. J. 1971; A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85
    [Google Scholar]
  4. Chakraborty R. A., Ahmed R., Fields B. N. 1979; Genetics of reovirus: the relationship of interference to complementation and reassortment of temperature-sensitive mutants at non permissive temperature. Virology 94:119–127
    [Google Scholar]
  5. Davies J. W. 1979 Translation of plant virus ribonucleic acids in extracts from eukaryotic cells. In Nucleic Acids in Plants vol. 2 pp. 111–148 Edited by Daviest J. W., Hall C. Florida: CRC Press;
    [Google Scholar]
  6. Dawson W. O. 1978; Isolation and mapping of replication-deficient, temperature-sensitive mutants of cowpea chlorotic mottle virus. Virology 90:112–118
    [Google Scholar]
  7. Dawson W. O. 1981; Effect of temperature-sensitive, replication-defective mutations on RNA synthesis of cow- pea chlorotic mottle virus. Virology 115:130–136
    [Google Scholar]
  8. Dawson W. O., Jones G. E. 1976; A procedure for specifically selecting temperature sensitive mutants of tobacco mosaic virus. Molecular and General Genetics 145:307–309
    [Google Scholar]
  9. Dawson W. O., White J. L. 1978; Characterization of a temperature-sensitive mutant of tobacco mosaic virus deficient in the synthesis of all RNA species. Virology 90:209–213
    [Google Scholar]
  10. Dawson W. O., White J. L. 1979; A temperature-sensitive mutant of tobacco mosaic virus deficient in synthesis of single stranded RNA. Virology 93:104–110
    [Google Scholar]
  11. De jager C. P. 1978 Genetische analyse van cowpea-mozaiek virusmutanten Ph.D. thesis Landbouwhogeschool Wageningen;
    [Google Scholar]
  12. Dingjan-versteegh A., Van vloten-doting L., Jaspars E. M. J. 1972; Alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. Virology 49:716–722
    [Google Scholar]
  13. Donis-keller H., Browning K. S., Clark J. M. JR. 1981; Sequence heterogeneity in satellite tobacco necrosis virus RNA. Virology 110:43–54
    [Google Scholar]
  14. Fulton R. W. 1952; Mutation in a tobacco necrosis virus strain. Phytopathology 42:156–158
    [Google Scholar]
  15. Heijtink R. A., Houwing C. J., Jaspars E. M. J. 1977; Molecular weights of particles and RNAs of alfalfa mosaic virus. Number of subunits in protein capsids. Biochemistry 16:4684–4693
    [Google Scholar]
  16. Heller E., Scholtissek C. 1980; Evidence for intracistronic complementation of the product of the influenza virus gene Pira (P3 of fowl plague virus). Journal of General Virology 49:133–139
    [Google Scholar]
  17. Honess R. W. 1981; Complementation between phosphonoacetic acid resistant and sensitive variants of herpes simplex viruses: evidence for an oligomeric protein with restricted intracellular diffusion as the determinant of resistance and sensitivity. Journal of General Virology 57:297–306
    [Google Scholar]
  18. Hull R., Plaskitt A. 1970; Electron microscopy on the behaviour of two strains of alfalfa mosaic virus in mixed infections. Virology 42:773–776
    [Google Scholar]
  19. Israël A. 1980; A temperature-dependent host range mutant of fowl plague virus (FPV). Virology 105:1–12
    [Google Scholar]
  20. Kiberstis P. A., Loesch-fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  21. Kowal K. J., Stollar V. 1981; Temperature-sensitive host dependent mutants of Sindbis virus. Virology 114:140–148
    [Google Scholar]
  22. Ledinko N. 1963; An analysis of interference between active polioviruses types 1 and 2 in HELA cells. Virology 20:29–44
    [Google Scholar]
  23. Lee H. H., Miller L. K. 1979; Isolation, complementation, and initial characterization of temperature sensitive mutants of the baculovirus Autographa califomica nuclear polyhedrosis virus. Journal of Virology 31:240252
    [Google Scholar]
  24. Matthews R. E. F. 1970 Variability. In Plant Virology pp. 379–418 Edited by Matthews R. E. F. New York: Academic Press;
    [Google Scholar]
  25. Matthews R. E. F. 1979; Classification and nomenclature of viruses. Third report of the international committee on taxonomy of viruses. Intervirology 12:129–296
    [Google Scholar]
  26. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
    [Google Scholar]
  27. Preble O. T., Youngner J. S. 1973; Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus. Journal of Virology 12:472–480
    [Google Scholar]
  28. Robinson D. J. 1973; Properties of two temperature-sensitive mutants of tobacco rattle virus. Journal of General Virology 21:499–506
    [Google Scholar]
  29. Sakai F., Dawson J. R. O., Watts J. W. 1979; Synthesis of proteins in tobacco protoplasts infected with brome mosaic virus. Journal of General Virology 42:323–328
    [Google Scholar]
  30. Schaffer P. A., Carter V. C., Timbury M. C. 1978; Collaborative complementation study of temperaturesensitive mutants of herpes simplex virus types 1 and 2. Journal of Virology 27:490–504
    [Google Scholar]
  31. Siegel A. 1959; Mutual exclusion of strains of tobacco mosaic virus. Virology 8:910–477
    [Google Scholar]
  32. Stollar V., Peleg J., Shenk T. E. 1974; Temperature sensitivity of a Sindbis virus mutant isolated from persistently infected Aedes aegypti cell culture. Intervirology 2:337–344
    [Google Scholar]
  33. Sugita K. 1981; Interference between virulent and avirulent strains of Sendai virus. Journal of General Virology 55:95–107
    [Google Scholar]
  34. Thierry F., Spring S. B. 1981; Localization of the ts defects of ts mutants of influenza virus using complementation analysis and gel analysis of the RNA segments of recombinants. Virology 115:137–148
    [Google Scholar]
  35. Thierry F., Spring S. B., Chanock R. M. 1980; Localization of the ts defect in two ts mutants of influenza virus: evidence for the occurrence of intracistronic complementation between ts mutants of influenza a virus coding for the neuraminidase and nucleoprotein polypeptides. Virology 101:484–492
    [Google Scholar]
  36. Van tol R. G. L. 1981 Expression of the alfalfa mosaic virus genome Ph.D. thesis Rijksuniversiteit Leiden;
    [Google Scholar]
  37. Van vloten-doting L., Neeleman L. 1982 Translation of plant virus RNAs. In Encyclopedia of Plant Physiology vol. 16 Edited by Boulter A., Parthier B. New YorkBerlin: Springer-Verlag (in press);
    [Google Scholar]
  38. Van vloten-doting L., Dingjan-versteegh A., Jaspars E. M. J. 1970; Three nucleoprotein components of alfalfa mosaic virus necessary for infectivity. Virology 40:419–430
    [Google Scholar]
  39. Van vloten-doting L., Hasrat J. A., Oosterwijk E., Van T sant P., Schoen I. A., Roosien J. 1980; Description and complementation analysis of 13 temperature-sensitive mutants of alfalfa mosaic virus. Journal of General Virology 46:415–426
    [Google Scholar]
  40. Van vloten-doting L., Francki R. I. B., Fulton R. W., Kaper J. M., Lane L. C. 1981; Tricomaviridae - a proposed family of plant viruses with tripartite, single-stranded RNA genomes. Intervirology 15:198–203
    [Google Scholar]
  41. Wu J., Rappaport I. 1961; An analysis of the interference between two strains of tobacco mosaic virus on Phaseolus vulgaris L. Virology 14:259–263
    [Google Scholar]
  42. Yarwood C. E. 1979; Host passage effects with plant viruses. Advances in Virus Research 25:169–187
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-63-1-189
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error