1887

Abstract

SUMMARY

The three serotypes of reovirus differ markedly in their response to a variety of chemical inactivating agents. We used intertypic recombinants containing various combinations of genes derived from the parental serotypes to study the basis of these differences. In addition to recombinants derived from types 1 and 3, and 2 and 3, we were able to isolate recombinants derived from types 1 and 2, suggesting that these two serotypes also undergo unrestricted reassortment. The intertypic recombinants behaved like one parent or the other in the presence of the inactivating agents and allowed us to determine the genes responsible for each difference. Recombinants derived from crosses between wild-type parental serotypes produced straightforward results, while recombinants derived from mutagenized, temperature-sensitive parents often did not. Sensitivity to 2.5 M-guanidine-HCl and pH 11 was determined by the SI gene, sensitivity to 55 °C and 1 % SDS was determined by the S4 gene, and sensitivity to 33% ethanol and to 1 % phenol was determined by the M2 gene. Thus, relatively nonspecific chemical agents appear to have their predominant effect on specific proteins of the reovirus virion.

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1982-11-01
2024-12-06
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References

  1. Ahmed R. A., Fields B. N. 1981; Reassortment of genome segments between reovirus defective interfering particles and infectious virus: construction of temperature-sensitive and attenuated viruses by rescue of mutations from DI particles. Virology 111:351–363
    [Google Scholar]
  2. Bitton G. 1980 Environmental Virology pp. 1–51 New York: John Wiley & Sons;
    [Google Scholar]
  3. Cooper P. D. 1977 Genetics of picomaviruses. In Comprehensive Virology 9133–207 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  4. Cross R. K., Fields B. N. 1976; Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3. Virology 74:345–362
    [Google Scholar]
  5. Davis B. D., Dulbecco R., Eisen H. N., Ginsberg H. S. (editors) 1980 Microbiology,3rd edn1264–1274 New York: Harper & Row;
    [Google Scholar]
  6. Drayna D., Fields B. N. 1982; Biochemical studies on the mechanism of chemical and physical inactivation of reovirus. Journal of General Virology 63:161–170
    [Google Scholar]
  7. Fenner F. 1958; The biological characters of several strains of vaccinia, cowpox, and rabbitpox viruses. Virology 5:502–529
    [Google Scholar]
  8. Fenner F., Mcauslan B. A., Mims C. A., Sambrook J., White D. O. (editors) 1974 The Biology of Animal Viruses, 2nd edn. p 22 New York: Academic Press;
    [Google Scholar]
  9. Fields B. N. 1981; Genetics of reovirus. Current Topics in Microbiology and Immunology 91:1–24
    [Google Scholar]
  10. Gard S. 1957 Chemical inactivation of viruses. Ciba Foundation Symposium on Nature of Viruses116–124 Edited by Wolstenholme G., Millar E. London: J. A. Churchill;
    [Google Scholar]
  11. Hrdy D. B., Rosen L., Fields B. N. 1979; Polymorphism of the migration of double-stranded RNA genome segments of reovirus isolates from humans, cattle, and mice. Journal of Virology 31:104–111
    [Google Scholar]
  12. Joklik W. K. 1972; Studies on the effect of Chymotrypsin on reovirions. Virology 49:700–715
    [Google Scholar]
  13. Joklik W. K. 1980; The structure and function of the reovirus genome. Annals of the New York Academy of Sciences 354:107–124
    [Google Scholar]
  14. Melnick J. L., Gerba C. P., Wallis C. 1978; Viruses in water. Bulletin of the World Health Organization 56:499–508
    [Google Scholar]
  15. Mustoe T. A., Ramig R. F., Sharpe A. H., Fields B. N. 1978a; A genetic map of reovirus. III. Assignment of the double-stranded RNA-positive mutant groups A, B, and G to genome segments. Virology 85:545–556
    [Google Scholar]
  16. Mustoe T. A., Ramig R. F., Sharpe A. H., Fields B. N. 1978b; Genetics of reovirus: identificaiton of the dsRNA segments encoding the polypeptides of the μ and σ size classes. Virology 89:594–604
    [Google Scholar]
  17. Ramig R. F., Cross R. K., Fields B. N. 1977; Genome RNA’s and polypeptides of reovirus serotypes 1,2 and 3. Journal of Virology 22:726–733
    [Google Scholar]
  18. Ramig R. F., Mustoe T. A., Sharpe A. H., Fields B. N. 1978; A genetic map of reovirus. II. Assignment of the double-stranded RNA-negative mutant groups, C, D, and E to genome segments. Virology 85:531–544
    [Google Scholar]
  19. Rubin D. H., Fields B. N. 1979; The molecular basis of reovirus virulence: the role of the M2 gene. Journal of Experimental Medicine 152:853–863
    [Google Scholar]
  20. Sharpe A. H., Fields B. N. 1982; Reovirus inhibition of cellular RNA and protein synthesis: role of the S4gene. Virology (in press)
    [Google Scholar]
  21. Sharpe A. H., Ramig R. F., Mustoe T. A., Fields B. N. 1978; A genetic map of reovirus. I. Correlation of genome RNAs between serotypes 1, 2, and 3. Virology 84:63–74
    [Google Scholar]
  22. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top component, and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  23. Wallis C., Melnick J. L. 1962; Cationic stabilization - a new property of enteroviruses. Virology 16:504–506
    [Google Scholar]
  24. Weiner H. L., Ramig R. F., Mustoe T. A., Fields B. N. 1978; Identification of the gene coding for the hemagglutinin of reovirus. Virology 86:581–584
    [Google Scholar]
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