1887

Abstract

SUMMARY

Attempts were made to change the envelope of murine sarcoma virus (MSV) from B-MuX (a xenotropic murine leukaemia virus isolate) to feline leukaemia virus (FeLV) by infecting cat cells with MSV(B-MuX), adding excess FeLV as helper and using mouse serum oncovirus-inactivating factor or anti-B-MuX serum. In several attempts, virus from single foci of MSV initially contained only MSV(FeLV) but on passage each showed a minimal persistence of B-MuX. To eliminate the B-MuX component, MSV(B-MuX) was subjected to two consecutive transspecies rescues. The first was performed by co-cultivation of MSV(B-MuX)-producing quail cells with mouse 3T3FL cells, which are completely non-permissive for B-MuX, and pure ecotropic Friend Eveline strain of murine leukaemia virus (F-MuLV); this resulted in apparently pure MSV(F-MuLV). Second, these MSV(F-MuLV)-infected 3T3FL cells were co-cultivated with FeLV and cat cells, which are completely nonpermissive for F-MuLV; this resulted in the generation of MSV(FeLV). Passage of this apparently pure FeLV pseudotype in cells permissive only for the replication of B-MuX surprisingly revealed residual murine xenotropic virus. It appears that pressure for survival resulted in genomic masking of B-MuX by heterologous virus envelopes. This phenomenon, which also occurs extensively in nature, implies that if absolute oncovirus genetic purity is required, even extensive attempts at purification may be inadequate.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-2-393
1982-10-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/2/JV0620020393.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-2-393&mimeType=html&fmt=ahah

References

  1. Fischinger P. J., O’connor T. E. 1969; Viral infection across species barriers: reversible alteration of murine sarcoma virus for growth in cat cells. Science 165:714–716
    [Google Scholar]
  2. Fischinger P. J., Nomura S., Blevins C. S. 1975; Two levels of restriction by mouse or cat cells of murine sarcoma virus coated by endogenous xenotropic oncornavirus. Journal of General Virology 29:51–62
    [Google Scholar]
  3. Fischinger P. J., Blevins C. S., Dunlop N. M. 1978; Genomic masking of nondefective recombinant murine leukemia virus in Moloney virus stocks. Science 201:457–459
    [Google Scholar]
  4. Fischinger P. J., Ihle J. N., Levy J. P., Bolognesi D. P., Elder J. H., Schafer W. 1981 Recombinant murine leukemia viruses and protective factors in leukemogenesis. In Viruses in Naturally Occurring Cancer vol. 7 pp. 989–1003 Edited by Essex M., Todaro G., zur Hausen H. New York; Cold Spring Harbor Laboratory:
    [Google Scholar]
  5. Frankel A. E., Neubauer R. L., Fischinger P. J. 1976; Fractionation of DNA nucleotide transcripts from Moloney sarcoma virus and isolation of sarcoma virus-specific complementary DNA. Journal of Virology 18:481–490
    [Google Scholar]
  6. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. 1966; Rescue of the defective genome of Moloney sarcoma virus from a non-infectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proceedings of the National Academy of Sciences of the United Slates of America 56:1164–1169
    [Google Scholar]
  7. Hunsman G., Moennig L., Pister E., Seifert E., Schafer W. 1974; Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology 62:307–318
    [Google Scholar]
  8. Ihle J. N., Fischinger P. J., Bolognesi D. P., Elder J. H., Gautsch J. W. 1978; B-MuX: a unique murine C-type virus containing the env gene of xenotropic viruses and the gag gene of the ecotropic virus. Virology 90:255–264
    [Google Scholar]
  9. Oskarsson M. K., Robey W. G., Harris C. L., Fischinger P. J., Haapala D. K., Vande Woude G. F. 1975; A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus p30 antigenic determinants. Proceedings of the National Academy of Sciences of the United States oj America 72:2380–2384
    [Google Scholar]
  10. Robey W. G., Oskarsson M. K., Vande Woude G. F., Naso R. B., Arlinghaus R. B., Haapala D. K., Fischinger P. J. 1977; Cells transformed by certain strains of Moloney sarcoma virus contain murine p60. Cell 10:79–89
    [Google Scholar]
  11. Robey W. G., Kuenzel W. J., Vande Woude G. F., Fischinger P. J. 1982; Growth of murine sarcoma and murine xenotropic leukemia viruses in Japanese quail. I. Induction of tumors and development of continuous tumor cell lines. Cancer Research (in press)
    [Google Scholar]
  12. Scolnick E. M., Howk R. S., Anisowicz A., Peebles P. T., Scher C. D., Parks W. P. 1975; Separation of sarcoma-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus. Proceedings oj the National Academy of Sciences of the United States of A merica 72:4650–4654
    [Google Scholar]
  13. Vogt P. J. 1977 Genetics of RNA tumor viruses. In Comprehensive Virology vol. 9 pp. 341–455 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-2-393
Loading
/content/journal/jgv/10.1099/0022-1317-62-2-393
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error