1887

Abstract

SUMMARY

Coliphage BA14 was isolated from sewage and shown to be related to phages T7 and T3. It is similar to T3 in that it directs the synthesis of an -adenosyl-methionine-cleaving enzyme (SAMase) early upon infection. However, it differs from all other known T7-related coliphages by the inability of its RNA polymerase (gene product) to transcribe T7 DNA or T3 DNA. BA14, T7 and T3 also show marked differences in autoradiographic patterns of their gel-electrophoretically separated S-labelled intracellular phage proteins, restriction endonuclease I cleavage patterns of their DNAs, and serological specificities of their infectious particles. Other distinctive features became apparent upon simultaneous mixed infection with BA14 and T7 or T3: inability of BA14 to produce genetic recombinants with either T7 or T3; lack of functional complementation between amber mutants of BA14 and T7 or T3; mutual exclusion and depression of the burst size of the mixedly infected cells.

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1982-10-01
2021-10-20
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References

  1. Beier H., Hausmann R. 1974; T3 × T7 phage crosses leading to recombinant RNA polymerases. Nature London 251:538–540
    [Google Scholar]
  2. Chamberlin M., McGrath J., Waskell L. 1970; New RNA polymerase from Escherichia coti infected with bacteriophage T7. Nature, London 228:227–231
    [Google Scholar]
  3. Dunn J. J., Studier F. W. 1981; Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. Journal of Molecular Biology 148:303–330
    [Google Scholar]
  4. Dunn J. J., Bautz F. A., Bautz E. K. F. 1971; Different template specificities of phage T3 and T7 RNA polymerases. Nature New Biology 230:94–96
    [Google Scholar]
  5. Gefter M., Hausmann R., Gold M., Hurwitz J. 1966; The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosyl-methionine cleavage. Journal of Biological Chemistry 241:1995–2005
    [Google Scholar]
  6. Gefter M. L., Becker A., Hurwitz J. 1967; The enzymatic repair of DNA. I. Formation of circular λ DNA. Proceedings of the National Academy of Sciences of the United States of America 58:240–247
    [Google Scholar]
  7. Hausmann R. 1976; Bacteriophage T7 genetics. Current Topics in Microbiology and Immunology 75:77–110
    [Google Scholar]
  8. Hausmann R., Gomez B. 1967; Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis. Journal of Virology 1:779–792
    [Google Scholar]
  9. Hausmann R. L., Almeida-Magalhaes E. P., Araujo C. 1962; Intracellular compatibility of coliphages T3, T7, and Cro. A nais de Microbiologia 10:43–62
    [Google Scholar]
  10. Hyman R. W., Brunovskis I., Summers W. C. 1974; A biochemical comparison of the related bacteriophages T7, ϕI, ϕII, W31, H and T3. Virology 57:189–206
    [Google Scholar]
  11. Korsten K. H., Tomkiewicz C., Hausmann R. 1979; The strategy of infection as a criterion for phylogenetic relationships of non-coli phages morphologically similar to phage T7. Journal of General Virology 43:57–73
    [Google Scholar]
  12. Ponta H., Rahmsdorf H. J., Pai S. H., Hirsch-Kauffmann M., Herrlich P., Schweiger M. 1974; Control of gene expression in bacteriophage T7: transcriptional controls. Molecular and General Genetics 134:281–294
    [Google Scholar]
  13. Rahmsdorf H. J., Pai S. H., Herrlich P., Roskoski R. Jr, Schweiger M., Studier F. W. 1974; Protein kinase induction in Escherichia coli by bacteriophage T7. Proceedings of the National Academy of Sciences of the United States of America 71:586–589
    [Google Scholar]
  14. Spoerel N., Herrlich P., Bickle T. A. 1979; A novel bacteriophage defence mechanism: the anti-restriction protein. Nature, London 278:30–34
    [Google Scholar]
  15. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  16. Studier F. W. 1979; Relationships among different strains of T7 and among T7-related bacteriophages. Virology 95:70–84
    [Google Scholar]
  17. Studier F. W., Movva N. R. 1976; SAMase gene of bacteriophage T3 is responsible for overcoming host restriction. Journal of Virology 19:136–145
    [Google Scholar]
  18. Studier F. W., Rosenberg A. H., Simon M. N., Dunn J. J. 1979; Genetic and physical mapping in the early region of bacteriophage T7 DNA. Journal of Molecular Biology 135:917–937
    [Google Scholar]
  19. Wienand U., Feix G. 1980; Zein specific restriction enzyme fragments of maize DNA. FEES Letters 116:14–16
    [Google Scholar]
  20. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  21. Zillig W., Fujiki H., Blum W., Janekovic D., Schweiger M., Rahmsdorf H. J., Ponta H., Hirsch-Kauffmann M. 1975; In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage T7-induced protein kinase. Proceedings of the National Academy of Sciences of the United States of America 72:2506–2510
    [Google Scholar]
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