1887

Abstract

SUMMARY

Monospecific polyclonal antisera were raised in guinea-pigs against the calf rotavirus polypeptides VP1, VP2, VP3* + 4*, VP4.2, VP6, VP7.1, VP7.2 and VP10. All of the antisera gave a similar pattern of cytoplasmic immunofluorescence in rotavirus-infected cells, but spots of fluorescence of varying intensity with different sera were seen over the nucleus. Immune precipitation, using to collect immune complexes, showed that VP2 was precipitated by antiserum to VP2 (α-VP2) and VP6 by α-VP6. α-VP7.1 and α-VP7.2 both precipitated the same range of proteins from infected cells (VP7, VP7.1 and VP7.2) or from virions (VP7.1 and VP7.2). VP10, either from virions or infected cells, was not precipitated by α-VP10. The only antiserum which efficiently neutralized infectivity was α-VP7.2. There were low levels of neutralization with α-VP10 (but the results varied from experiment to experiment) and traces with α-VP6, α-VP7.1 and the other antisera did not neutralize even though α-VP7.1 agglutinated double-shelled particles as seen in immune electron microscopy to a greater extent than α-VP7.2 Both VP7.1 and VP7.2 were shown to be glycoproteins by tunicamycin treatment of infected cells. Core particles only were agglutinated by α-VP10. All the evidence leads us to conclude that there were major neutralizing antigenic determinants present on VP7.2, a minor component of the outer shell of the virion.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-2-297
1982-10-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/2/JV0620020297.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-2-297&mimeType=html&fmt=ahah

References

  1. Bastardo J. W., Mckimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. 1981; Preparation and characterization of antisera to electrophoretically purified SA 11 virus polypeptides. Infection and Immunity 34:641–647
    [Google Scholar]
  2. Beards G. M., Pilfold J. N., Thouless M. E., Flewett T. H. 1980; Rotavirus serotypes by serum neutralization. Journal of Medical Virology 5:231–237
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Bridger J. C., Clarke I. N., McCrae M. A. 1982; Characterization of an antigenically distinct porcine rotavirus. Infection and Immunity (in press)
    [Google Scholar]
  5. Clark S. M., Roth J. R., Clark M. L., Barnett B. B., Spendlove R. S. 1981; Trypsin enhancement of rotavirus infectivity: mechanism of enhancement. Journal of Virology 39:816–822
    [Google Scholar]
  6. Cook R. F., Avery R. J., Dimmock N. J. 1979; Infection of chicken erythrocytes with influenza and other viruses. Infection and Immunity 25:396–402
    [Google Scholar]
  7. Dyaldsmith M. L., Holmes I. H. 1981a; Gene coding assignments of rotavirus double-stranded RNA segments 10 and 11. Journal of Virology 38:1099–1133
    [Google Scholar]
  8. Dyall-Smith M. L., Holmes I. H. 1981b; Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. Journal of Virology 40:720–728
    [Google Scholar]
  9. Espejo R. T., Lopez S., Arias C. 1981; Structural polypeptides of simian rotavirus SA11 and the effect of trypsin. Journal of Virology 37:156–160
    [Google Scholar]
  10. Estes M. K., Graham D. Y., Mason B. B. 1981; Proteolytic enhancement of rotavirus infectivity. molecular mechanisms. Journal of Virology 39:879–888
    [Google Scholar]
  11. Faulkner-Valle G. P., Clayton A. V., McCrae M. A. 1982; Molecular biology of rotaviruses. III. Isolation and characterization of temperature sensitive mutants of bovine rotavirus. Journal of Virology (in press)
    [Google Scholar]
  12. Flewett T. H., Thouless M. E., Pilfold J. N., Bryden A. S., Candeias J. A. N. 1978; More serotypes of human rotavirus. Lancet ii:632
    [Google Scholar]
  13. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. 1981; Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proceedings of the National Academy of Sciences of the United States of America 78:420–424
    [Google Scholar]
  14. Hayes E. C., Lee P. W. K., Miller S. J., Joklik W. K. 1981; The interaction of a series of hybridoma IgG’s with reovirus particles. Demonstration that the core protein 12 is exposed on the particle surface. Virology 108:147–155
    [Google Scholar]
  15. Kalica A. R., Theodore T. S. 1979; Polypeptides of simian rotavirus (SA-11) determined by a continuous polyacrylamide gel electrophoresis method. Journal of General Virology 43:463–466
    [Google Scholar]
  16. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  17. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981; Genes of human (strain Wa) and bovine (strain U.K.) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  18. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 221:680–685
    [Google Scholar]
  20. McCrae M. A., Faulkner-Valle G. P. 1981; Molecular biology of rotaviruses. 1. Characterization of basic growth parameters and pattern of macromolecular synthesis. Journal of Virology 39:490–496
    [Google Scholar]
  21. McNulty M. S., Allan G. M., Todd D., McFerran J. B. 1979; Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Archives of Virology 61:13–21
    [Google Scholar]
  22. McNulty M. S., Allen G. M., Todd D., McFerran J. B., McCracken R. M. 1981; Isolation from chickens of a rotavirus lacking the rotavirus group antigen. Journal of General Virology 55:405–413
    [Google Scholar]
  23. Marchalonis J. J. 1969; An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal 113:299–305
    [Google Scholar]
  24. Matsuno S., Mukoyama A. 1979; Polypeptides of bovine rotavirus. Journal of General Virology 43:309–316
    [Google Scholar]
  25. Novo E., Esparza J. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  26. Rodger S. M., Schnagl R. D., Holmes I. H. 1975; Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  27. Saif L. J., Bohl E. H., Thiel K. W., Cross R. H., House J. A. 1980; Rotavirus-like, calicivirus-like and 23nm virus-like particles associated with diarrhoea in young pigs. Journal of Clinical Microbiology 12:106–114
    [Google Scholar]
  28. Schwarz R. T., Rohrschneider J. M., Schmidt M. F. 1976; Suppression of glycoprotein formation of Semliki Forest, influenza and avian sarcoma virus by tunicamycin. Journal of Virology 19:782–791
    [Google Scholar]
  29. Smith M. L., Lazdins I., Holmes I. H. 1980; Coding assignments of double stranded RNA segments of SAI 1 rotavirus established by in vitro translation. Journal of Virology 33:976–982
    [Google Scholar]
  30. Syvanen J. M., Yang Y. R., Kirschner M. W. 1973; Preparation of 125I catalytic subunit of aspartate transcarbamylase and its use in studies of the regulatory subunit. Journal of Biological Chemistry 248:3762–3768
    [Google Scholar]
  31. Thouless M. E. 1979; Rotavirus polypeptides. Journal of General Virology 44:187–197
    [Google Scholar]
  32. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. 1977; Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Archives of Virology 53:287–294
    [Google Scholar]
  33. Thouless M. E., Bryden A. S., Flewett T. H. 1978; Serotypes of human rotavirus. Lancet i:39
    [Google Scholar]
  34. Weiner A. L., Fields B. N. 1977; Neutralization of reovirus: the gene responsible for the neutralization antigen. Journal of Experimental Medicine 146:1305–1310
    [Google Scholar]
  35. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Bryden A. S., Davies H. A., White G. B. B. 1976; Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice and foals. Infection and Immunity 14:804–810
    [Google Scholar]
  36. Yolken R. H., Barbour R. G., Wyatt R. G., Kalica A. R., Kapikian A. Z., Chanock R. M. 1978; Enzyme linked immunosorbent assay for identification of rotaviruses from different animal species. Science 201:259–262
    [Google Scholar]
  37. Zissis G., Lambert J. P. 1978; Different serotypes of human rotaviruses. Lancet i:38–39
    [Google Scholar]
  38. Zissis G., Lambert J. P. 1980; Enzyme linked immunosorbent assay adapted for serotyping of human rotavirus strains. Journal of Clinical Microbiology 11:1–5
    [Google Scholar]
  39. Zissis G., Lambert J. P., Kapsenberg J. G., Enders G., Mutanda L. N. 1981; New human rotavirus serotypes. Lancet i:944–945
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-2-297
Loading
/content/journal/jgv/10.1099/0022-1317-62-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error