1887

Abstract

SUMMARY

Urea acts to disassemble tobacco mosaic virus (TMV) predominantly from the 5'-end of the virus RNA. Extensively uncoated particles exhibit some degree of bi-directional uncoating as judged by a reduced ability to bind [H]histidine. The translational activities of the RNA fragments which remained encapsidated in the urea-resistant fraction of TMV rods were investigated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-1-137
1982-07-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/1/JV0610010137.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-1-137&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Buzzell A. 1960; Action of urea on tobacco mosaic virus. Journal of the American Chemical Society 82:1636–1641
    [Google Scholar]
  3. Buzzell A. 1962; Action of urea on tobacco mosaic virus. II. The bonds between protein subunits. Biophysical Journal 2:223–233
    [Google Scholar]
  4. Carriquiry E., Litvak S. 1974; Further studies on the enzymatic aminoacylation of TMV-RNA by histidine. FEBS Letters 38:287–291
    [Google Scholar]
  5. Glover J. F., Wilson T. M. A. 1981; The efficient expression of a eukaryotic gene in a prokaryotic cell-free system. Biochemical Society Transactions 9:201 p.
    [Google Scholar]
  6. Glover J. F., Wilson T. M. A. 1982; Efficient translation of the coat protein cistron of tobacco mosaic virus in a cell-free system from Escherichia coli. European Journal of Biochemistry 122:485–492
    [Google Scholar]
  7. Guilley H., Jonard G., Kukla B., Richards K. E. 1979; Sequence of 1000 nucleotides at the 3'-end of tobacco mosaic virus RNA. Nucleic Acids Research 6:1287–1308
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  10. May D. S., Knight C. A. 1965; Polar stripping of protein subunits from tobacco mosaic virus. Virology 25:502–507
    [Google Scholar]
  11. Nicolaieff A., Lebeurier G. 1979; Polar uncoating of tobacco mosaic virus (TMV) with dimethylsulphoxide (DMSO) and subsequent reassembly of partially stripped TMV. Molecular and General Genetics 171:327–333
    [Google Scholar]
  12. Obero B., Philipson L. 1972; Binding of histidine to tobacco mosaic virus RNA. Biochemical and Biophysical Research Communications 48:927–932
    [Google Scholar]
  13. Ohno T., Okada Y. 1977; Polarity of stripping of tobacco mosaic virus by alkali and sodium dodecyl sulphate. Virology 76:429–432
    [Google Scholar]
  14. Pelcher L. E., Halasa M. c. 1979; Factors influencing the production of intermediate particles during alkaline degradation of tobacco mosaic virus: time, pH, salt concentration and temperature. Journal of Virology 29:431–437
    [Google Scholar]
  15. Perham R. N., Wilson T. M. A. 1976; The polarity of stripping of coat protein subunits from the RNA in tobacco mosaic virus under alkaline conditions. FEBS Letters 62:11–15
    [Google Scholar]
  16. Perham R. N., Wilson T. M. A. 1978; The characterization of intermediates formed during the disassembly of tobacco mosaic virus at alkaline pH. Virology 84:293–302
    [Google Scholar]
  17. Richards K. E., Guilley H., Jonard G., Hirth L. 1978; Nucleotide sequence at the 5'-extremity of tobacco mosaic virus RNA. European Journal of Biochemistry 84:513–519
    [Google Scholar]
  18. Roberts B. E., Paterson B. M. 1973; Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proceedings of the National Academy of Sciences of the United States of America 70:2330–2334
    [Google Scholar]
  19. Wilson T. M. A. 1978; The polarity of stripping of coat protein subunits from the RNA in tobacco mosaic virus by dimethylsulphoxide. FEBS Letters 87:17–20
    [Google Scholar]
  20. Wilson T. M. A., Perham R. N., Finch J. T., Butler P. J. G. 1976; Polarity of the RNA in the tobacco mosaic virus particle and the direction of protein stripping in sodium dodecyl sulphate. FEBS Letters 64:285–289
    [Google Scholar]
  21. Wilson T. M. A., Perham R. N., Butler P. J. G. 1978; Intermediates in the disassembly of tobacco mosaic virus at alkaline pH. Infectivity, self-assembly and translational activities. Virology 89:475–483
    [Google Scholar]
  22. Wilson T. M. A., Lomonossoff G. P., Glover J. F. 1981; Dimethyl sulphoxide (DMSO) disassembles tobacco mosaic virus predominantly from the 5'-end of the viral RNA. Journal of General Virology 53:225–234
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-61-1-137
Loading
/content/journal/jgv/10.1099/0022-1317-61-1-137
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error