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Volume 61, Issue 1

Early and Delayed Shut-off of Host Protein Synthesis in Cells Infected with Herpes Simplex Virus Free

Abstract

SUMMARY

A mutant of herpes simplex virus type 1 [HSV- 1(HFEM)], B7, appears to have two temperature-sensitive functions. One is required during the first hour of infecting a cell (suggesting that it is performed by a virion protein) and the other is the non-essential function of ‘early shut-off’ of cellular protein synthesis, which is mediated by a virion protein. The latter function remained temperature-sensitive in a revertant virus (RC2) grown at the non-permissive temperature (39 °C). However, under these conditions RC2 did cause inhibition of host synthesis, showing that ‘delayed shut-off’, requiring virus protein synthesis, can occur independently of early shut-off, which is mediated by a virion protein. Early shut-off by u.v.-irradiated B7 was reversed when the temperature was raised, whereas delayed shut-off by intact B7 was not. Of two wild-type strains of virus examined, HSV-1 (F) also exhibited temperature-sensitive early shut-off, but HSV-2(G) did not.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HSV , protein synthesis , shut-off and ts mutant
© Society for General Microbiology 1982
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1982-07-01
2024-04-26
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Early and Delayed Shut-off of Host Protein Synthesis in Cells Infected with Herpes Simplex Virus
J. Gen. Virol. 61, 121 (1982); https://doi.org/10.1099/0022-1317-61-1-121
/content/journal/jgv/10.1099/0022-1317-61-1-121
/content/journal/jgv/10.1099/0022-1317-61-1-121
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