1887

Abstract

SUMMARY

As demonstrated previously, a ‘beta particle’ fraction isolated from the cytoplasm of PLC/PRF/5 cells contains hepatitis B virus (HBV)-specific DNA. Here, further evidence is provided that the specificity of the DNA for HBV is represented at least by sequences coding for the surface and core antigen (HBsAg and HBcAg). This was shown by two different hybridization techniques. One of them, the technique of Southern, distinguished these hybrid molecules formed from those containing HBV DNA integrated into chromosomes. The HBV-specific beta particle DNA forms two distinct bands separate from the high molecular weight cellular DNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-61-1-105
1982-07-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/61/1/JV0610010105.html?itemId=/content/journal/jgv/10.1099/0022-1317-61-1-105&mimeType=html&fmt=ahah

References

  1. Brechot C., Pourcel C., Louise A., Rain B., Tiollais P. 1980; Presence of integrated hepatitis B virus DNA sequences in cellular DNA of human hepatocellular carcinoma. Nature, London 286:533–535
    [Google Scholar]
  2. Burrell C. J., Mackay P., Greenaway P. J., Hofschneider P. H., Murray K. 1979; Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR 322. Nature, London 279:43–47
    [Google Scholar]
  3. Chakraborty P. R., Ruiz-Opazo N., Shouval D., Shafritz D. A. 1980; Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line. Nature, London 286:531–533
    [Google Scholar]
  4. Cummings I. W., Browne J. K., Salser W. A., Tyler G. V., Snyder R. L., Smolec J. M., Summers J. 1980; Isolation, characterization, and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus. Proceedings of the National Academy of Sciences of the United States of America 77:1842–1846
    [Google Scholar]
  5. Edman J. C., Gray P., Valenzuela P., Rall L. B., Rutter W. J. 1980; Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature, London 286:535–538
    [Google Scholar]
  6. Koshy R., Maupas P., Mueller R., Hofschneider P. H. 1981; Detection of hepatitis B virus-specific DNA in the genomes of human hepatocellular carcinoma and liver cirrhosis tissues. Journal of General Virology 57:95–102
    [Google Scholar]
  7. Macnab G., Alexander J., Lecatsas G., Bey E., Urbanowicz J. 1976; Hepatitis B surface antigen produced by a human hepatoma cell line. British Journal of Cancer 34:509–515
    [Google Scholar]
  8. Marion P. L., Salazar F. H., Alexander J. J., Robinson W. S. 1980; State of hepatitis B viral DNA in a human hepatoma cell line. Journal of Virology 33:795–806
    [Google Scholar]
  9. Reitz M. S., Abrell J. W., Trainor C. D., Gallo R. C. 1972; Precipitation of nucleic acids with cetyltrimethylammonium bromide: a method for preparing viral and cellular DNA polymerase products for cesium sulfate density gradient analysis. Biochemical and Biophysical Research Communications 49:30–38
    [Google Scholar]
  10. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  11. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  12. Tanaka T., Weisblum B. 1975; Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. Journal of Bacteriology 121:354–362
    [Google Scholar]
  13. Zaslavsky V., Marquardt O., Wong T.-K., Hofschneider P. H. 1980; Hepatitis B virus (HBV)-specific structures found in cytoplasmic extracts of cells producing HBV surface antigen (HBsAg) in vitro. Journal of General Virology 51:341–349
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-61-1-105
Loading
/content/journal/jgv/10.1099/0022-1317-61-1-105
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error