1887

Abstract

SUMMARY

Tracheal organ cultures infected with mouse cytomegalovirus (MCMV) were shown to produce virus for up to 180 days with virus replication primarily in epithelial cells. Persistent virus infections were established in tracheal organ cultures from both MCMV-susceptible and MCMV-resistant strains of mice. In acutely infected tracheal organ cultures, cellular DNA synthesis appeared to precede the production of significant amounts of virus antigens and the release of virus into culture fluids. Since persistently infected tracheal organ cultures continued to synthesize cellular DNA, the results suggest that host cell turnover may continually renew the population of MCMV-susceptible cells. The results suggest a possible mechanism for virus persistence in tracheal organ culture based on continuing serial infection of newly susceptible cells combined with prolonged release of virus from infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-60-2-247
1982-06-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/60/2/JV0600020247.html?itemId=/content/journal/jgv/10.1099/0022-1317-60-2-247&mimeType=html&fmt=ahah

References

  1. Benyesh-Melnick M. 1969; Cytomegalovirus. In Diagnostic Procedures for Viral and Rickettsial Infections pp 701–732 Edited by Lennette E. H., Schmidt N. J. New York: American Public Health Association;
    [Google Scholar]
  2. Boren H. G., Paradise L. J. 1978; Cytokinetics of lung. In Pathogenesis and Therapy of Lung Cancer pp 369–413 Edited by Harris C. C. New York: Marcel Dekker;
    [Google Scholar]
  3. Brautigam A. R., Dutko F. J., Olding L. B., Oldstone M. B. A. 1979; Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency. Journal of General Virology 44:349–359
    [Google Scholar]
  4. Cheeseman S. H., Stewart J. A., Winkle S., Cosimi A. B., Tolkoff-Rubin N. E., Russell P. S., Baker G. P., Herrin J., Rubin R. H. 1979; Cytomegalovirus excretion 2–14 years after renal transplantation. Transplantation Proceedings 9:71–74
    [Google Scholar]
  5. Demarchi J. M., Kaplan A. S. 1976; Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. Journal of Virology 18:1063–1070
    [Google Scholar]
  6. Demarchi J. M., Kaplan A. S. 1977; Physiological state of human embryonic lung cells affects their response to human cytomegalovirus. Journal of Virology 23:126–132
    [Google Scholar]
  7. Dutko F. J., Oldstone M. B. A. 1981; Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. Journal of Experimental Medicine 154:1636–1651
    [Google Scholar]
  8. Furakawa T. 1979; Persistent infection with human cytomegalovirus in a lymphoblastoid cell line. Virology 94:214–218
    [Google Scholar]
  9. Grundy (Chalmer) J. E., Mackenzie J. S., Stanley N. F. 1981; Influence of H-2 and non H-2 genes on resistance of murine cytomegalovirus infection. Infection and Immunity 23:277–286
    [Google Scholar]
  10. Hanshaw J. B. 1971; Congenital cytomegalovirus infection: a fifteen year perspective. Journal of Infectious Diseases 123:555–561
    [Google Scholar]
  11. Henson D., Neapolitan C. 1970; Pathogenesis of chronic mouse cytomegalovirus infection in submaxillary glands of C3H mice. American Journal of Pathology 58:255–267
    [Google Scholar]
  12. Henson D., Smith R. D., Gehrke J., Neapolitan C. 1967; Effect of cortisone on nonfatal mouse cytomegalovirus infection. American Journal of Pathology 5 1:1001–1011
    [Google Scholar]
  13. Jordan M. C. 1978; Interstitial pneumonia and subclinical infection after intranasal inoculation of murine cytomegalovirus. Infection and Immunity 21:275–280
    [Google Scholar]
  14. Knowles W. A. 1976; In vitro cultivation of human cytomegalovirus in thyroid epithelial cells. Archives of Virology 50:119–124
    [Google Scholar]
  15. Light J. A., Burke D. S. 1979; Association of cytomegalovirus (CMV) infections with increased recipient mortality following transplantation. Transplantation Proceedings 11:79–82
    [Google Scholar]
  16. Mäntyjärvi R. A., Selgrade M. J., Collier A. M., Hu S., Pagano J. S. 1977; Murine cytomegalovirus infection of epithelial cells in mouse tracheal ring organ culture. Journal of Infectious Diseases 136:444–448
    [Google Scholar]
  17. Michelson-Fiske S., Arnoult J., Febvre H. 1975; Cytomegalovirus infection of human lung epithelial cells in vitro. Intervirology 5:354–363
    [Google Scholar]
  18. Mims C. A. 1974; Factors in the mechanism of persistence of viral infections. Progress in medical Virology 18:1–14
    [Google Scholar]
  19. Mims C. A., Gould J. 1978; The role of macrophages in mice infected with murine cytomegalovirus. Journal of General Virology 41:143–153
    [Google Scholar]
  20. Mims C. A., Gould J. 1979; Infection of salivary gland, kidney, adrenals, ovaries and epithelia by murine CMV. Journal of Medical Microbiology 12:113–121
    [Google Scholar]
  21. Mocarski E. S., Stinski M. F. 1979; Persistence of the cytomegalovirus genome in human cells. Journal of Virology 31:761–775
    [Google Scholar]
  22. Muller M. T., Hudson J. B. 1977; Cell cycle dependency of murine-CMV replication in synchronized 3T3 cells. Journal of Virology 22:267–272
    [Google Scholar]
  23. Nedrud J. G., Collier A. M., Pagano J. S. 1979; Cellular basis for susceptibility to mouse cytomegalovirus: evidence from tracheal organ culture. Journal of General Virology 45:737–744
    [Google Scholar]
  24. Neiman P. E., Reeves W., Ray G., Flournay N., Lerner K. G., Sale G. E., Thomas E. D. 1977; A prospective analysis of interstitial pneumonia and opportunistic viral infection among recipients of allogeneic bone marrow grafts. Journal of Infectious Diseases 136:754–767
    [Google Scholar]
  25. Olding L. B., Jensen F. C., Oldstone M. B. A. 1975; Pathogenesis of cytomegalovirus infection. I. Activation of virus from bone-marrow derived lymphocytes by in vitro allogeneic reaction. Journal of Experimental Medicine 141:561–572
    [Google Scholar]
  26. Osborne J. 1982; CMV-herpesviruses of mice. In The Mouse in Biomedical Research vol 2: Edited by Foster H. L., Fox J. G., Small J. D. New York: Academic Press; (in press)
    [Google Scholar]
  27. Peterson P. K., Balfour H. H., Marker S. C., Fryd D. S., Howard R. J., Simmons R. L. 1980; Cytomegalovirus disease in renal allograft recipients: a prospective study of the clinical features, risk factors and impact of renal transplantation. Medicine 59:283–300
    [Google Scholar]
  28. Reynolds D. W., Stagno S., Stubbs G., Dahle A. J., Livingston M. M., Saxon S. S., Alford C. A. 1974; Inapparent congenital cytomegalovirus infection with elevated cord IgM levels: causal relation with auditory and mental deficiency. New England Journal of Medicine 290:291–296
    [Google Scholar]
  29. Vesterinen E., Leinikki P., Saksela E. 1975; Cytopathogenicity of cytomegalovirus to human ecto-and endo-cervical epithelial cells in vitro. Acta Cytologica 19:473–481
    [Google Scholar]
  30. Vonka V., Anisimova E., Macek M. 1976; Replication of cytomegalovirus in human epithelioid diploid cell line. Archives of Virology 58:283–296
    [Google Scholar]
  31. Weller T. H. 1971; The cytomegalovirus: ubiquitous agents with protean clinical manifestations. New England Journal of Medicine 285: 203–214 267–274
    [Google Scholar]
  32. Wu B. C., Ho M. 1979; Characteristics of infection of B and T lymphocytes from mice after inoculation with cytomegalovirus. Infection and Immunity 24:856–864
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-60-2-247
Loading
/content/journal/jgv/10.1099/0022-1317-60-2-247
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error