1887

Abstract

SUMMARY

Human rotaviruses (HRVs) derived from stools were cultivated to high titres in an established cell line (MA-104) using the rotary culture system. Analysis of the 11 double-stranded RNA segments of the culture-adapted HRVs was carried out by polyacrylamide gel electrophoresis. Tissue culture-adapted HRVs, uncloned or cloned, had the same RNA gel patterns as those of the original HRVs from the individual stool specimens. The migrations of the RNA segments from the culture-adapted HRVs were markedly different from that of the RNA segments from calf rotavirus (Lincoln strain). Considerable heterogeneity in electrophoretic migration of the RNA was found among eight strains of the HRVs grown in cell culture. These results confirmed that viruses isolated from stool specimens were indeed of human origin and were not the result of laboratory contamination with tissue culture-adapted calf rotavirus or other viruses.

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/content/journal/jgv/10.1099/0022-1317-60-1-171
1982-05-01
2021-10-16
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References

  1. Espejo R. T., Calderón E., Gonzalez N., Sálomon A., Martuscelli A., Romero P. 1979; Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City, 1977. Journal of Infectious Diseases 139:474–477
    [Google Scholar]
  2. Espejo R. T., Muñóz F., Serafin F., Romero P. 1980; Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Journal of Virology 30:839–846
    [Google Scholar]
  3. Flewett T. H., Woode G. N. 1978; The rotaviruses. A rchives of Virology 57:1–23
    [Google Scholar]
  4. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Van Kirle D. H., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  5. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981; Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proceedings of the National Academy of Sciences of the United States of America 78:420–424
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Rodger S. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  8. Rodger S. M., Bishop R. F., Birch C., Mclean B., Holmes I. H. 1981; Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  9. Sato K., Inaba Y., Shinozaki T., Fujii R., Matsumoto M. 1981; Isolation of human rotavirus in cell cultures. Archives of Virology 69:155–160
    [Google Scholar]
  10. Urasawa T., Urasawa S., Taniguchi K. 1981; Sequential passages of human rotavirus in MA-104 cells. Microbiology and Immunology 25:1025–1035
    [Google Scholar]
  11. Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. J. 1980; Human rotavirus type 2: cultivation in vitro. Science 207:189–191
    [Google Scholar]
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