Intranuclear paracrystalline bodies have been observed in cells infected with adenovirus type 5 (Leuchtenberger & Boyer, 1957; Morgan 1957, 1960). The present study reports the attempts to isolate these paracrystalline proteins from KB cell nuclei and discusses their nature and possible role.

KB cells were grown as monolayers in 1 l. bottles (5 × 10 to 6 × 10 cells/bottle) in Eagle's basal medium supplemented with 10% calf serum. The cells were infected at a multiplicity of 100/infecting cell units/cell (Warocquier, Ménard & Samaille, 1966) and the virus was allowed to grow for 48 hr. The paracrystalline bodies were extracted directly from infected KB cells by homogenizing them in 1% (w/v) citric acid (Mirsky & Pollister, 1946) or 0.15% (v/v) Cemulsol (Zalta 1962) using a Dounce homogenizer. The suspension was centrifuged at 1000 for 10 min. and the pellet examined in the electron microscope, after fixation in glutaraldehyde + osmium tetroxide and embedding in Araldite (Richardson, Jarett & Finke, 1960).


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