1887

Abstract

Intranuclear paracrystalline bodies have been observed in cells infected with adenovirus type 5 (Leuchtenberger & Boyer, 1957; Morgan 1957, 1960). The present study reports the attempts to isolate these paracrystalline proteins from KB cell nuclei and discusses their nature and possible role.

KB cells were grown as monolayers in 1 l. bottles (5 × 10 to 6 × 10 cells/bottle) in Eagle's basal medium supplemented with 10% calf serum. The cells were infected at a multiplicity of 100/infecting cell units/cell (Warocquier, Ménard & Samaille, 1966) and the virus was allowed to grow for 48 hr. The paracrystalline bodies were extracted directly from infected KB cells by homogenizing them in 1% (w/v) citric acid (Mirsky & Pollister, 1946) or 0.15% (v/v) Cemulsol (Zalta 1962) using a Dounce homogenizer. The suspension was centrifuged at 1000 for 10 min. and the pellet examined in the electron microscope, after fixation in glutaraldehyde + osmium tetroxide and embedding in Araldite (Richardson, Jarett & Finke, 1960).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-6-2-329
1970-02-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-6-2-329
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error