1887

Abstract

SUMMARY

Adenovirus type 12 (Ad12)-specific proteins were determined in nine well-characterized Ad12-transformed hamster cell lines and in Ad12-induced hamster and rat tumour lines by immunoprecipitation, gel electrophoresis and autoradiography. All cell lines expressed a 60K mol. wt. polypeptide. In several cell lines, the presence of a 58K mol. wt. protein could also be demonstrated by these techniques. Smaller Ad12-specific proteins could be detected only in the Ad12-transformed hamster cell lines HA12/7 and A2497-3, and in the Ad12-induced hamster tumour line CLAC3. The quality of the immunoprecipitation tests performed depended primarily on the sera used; their antibody titres varied widely. For at least one of the Ad12-transformed hamster cell lines (HA12/7), there was good agreement in the number and mol. wt. of Ad12-specific proteins detected by both immunoprecipitation and translation of hybrid-selected cytoplasmic RNA. There was no clear-cut correlation between the number and the nature of Ad12-specific proteins and the way in which the cell lines or tumours were obtained. Cell lines with the least number of copies of Ad12 DNA persisting appeared to express the largest number of Ad12-specific proteins.

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1982-04-01
2022-01-18
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References

  1. Bablanian R., Eggers H., Tamm I. 1965; Studies on the mechanism of poliovirus-induced cell damage. I. The relation between poliovirus-induced metabolic and morphological alterations in cultured cells. Virology 26:100–113
    [Google Scholar]
  2. Crawford L. V., Pim D. C., Lane D. P. 1980; An immunological investigation of SV40 T-antigens. 2. Quantitation of antigens and antibody activities. Virology 100:314–325
    [Google Scholar]
  3. Deuring R., Winterhoff U., Tamanoi F., Stabel S., Doerfler W. 1981; Site of linkage between adenovirus type 12 and cell DNAs in hamster tumour line CLAC3. Nature, London 293:81–84
    [Google Scholar]
  4. Doerfler W. 1969; Nonproductive infection of baby hamster kidney cells (BHK 21) with adenovirus type 12. Virology 38:587–606
    [Google Scholar]
  5. Doerfler W., Stabel S., Ibelgaufts H., Sutter D., Neumann R., Groneberg J., Scheidtmann K. H., Deuring R., Winterhoff U. 1979; Selectivity in integration sites of adenoviral DNA. Cold Spring Harbor Symposia on Quantitative Biology 44:551–564
    [Google Scholar]
  6. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
    [Google Scholar]
  7. Edvardsson B., Ustacelebi S., Williams J., Philipson L. 1978; Assembly intermediates among adenovirus type 5 temperature-sensitive mutants. Journal of Virology 25:641–651
    [Google Scholar]
  8. Eick D., Doerfler W. 1982; Integrated adenovirus type 12 DNA in the transformed hamster cell line T637: sequence arrangements at the termini of viral DNA and mode of amplification. Journal of Virology 42: (in press)
    [Google Scholar]
  9. Eick D., Stabel S., Doerfler W. 1980; Revertants of adenovirus type 12-transformed hamster cell line T637 as tools in the analysis of integration patterns. Journal of Virology 36:41–49
    [Google Scholar]
  10. Esche H. 1982; Viral genes expressed in adenovirus type 2-transformed hamster cells. Journal of Virology 41: (in press)
    [Google Scholar]
  11. Esche H., Siegmann B. 1982; The expression of early viral genes in adenovirus type 12-infected and -transformed cells. Journal of General Virology (in press)
    [Google Scholar]
  12. Esche H., Schilling R., Doerfler W. 1979; In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells. Journal of Virology 30:21–31
    [Google Scholar]
  13. Groneberg J., Chardonnet Y., Doerfler W. 1977; Integrated viral sequences in adenovirus type 12-transformed hamster cells. Cell 10:101–111
    [Google Scholar]
  14. Ibelgaufts H., Doerfler W., Scheidtmann K.-H., Wechsler W. 1980; Adenovirus type 12-induced rat tumor cells of neuroepithelial origin: persistence and expression of the viral genome. Journal of Virology 33:423–437
    [Google Scholar]
  15. Jochemsen H., Daniels G., Lupker J. H., Van Der Eb A. J. 1980; Identification and mapping of the early gene products of adenovirus type 12. Virology 105:551–563
    [Google Scholar]
  16. Kuhlmann I., Doerfler W. 1982; Shifts in the extent and pattern of DNA methylation upon explantation and subcultivation of adenovirus type 12-induced hamster tumor cells. Virology 118: (in press)
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Ortin J., Scheidtmann K.-J., Greenberg R., Westphal M., Doerfler W. 1976; Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. Journal of Virology 20:355–372
    [Google Scholar]
  19. Ouchterlony O. 1958; Diffusion-in-gel methods for immunological analysis. Progress in Allergy 5:1–78
    [Google Scholar]
  20. Schirm S., Doerfler W. 1981; The expression of viral DNA in adenovirus type 12-transformed cells and in revertants. Journal of Virology 39:694–702
    [Google Scholar]
  21. Schrier P. J., Van Der Elsen P. J., Hertoghs J. J. L., Van Der Eb A. J. 1979; Characterization of tumor antigens in cells transformed by fragments of adenovirus type 5 DNA. Virology 99:372–385
    [Google Scholar]
  22. Shiroki K., Segawa K., Saito I., Shimojo H., Fujinaga K. 1979; Products of the adenovirus-12 transforming genes and their functions. Cold Spring Harbor Symposia on Quantitative Biology 44:533–540
    [Google Scholar]
  23. Stabel S., Doerfler W., Friis R. R. 1980; Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. Journal of Virology 36:22–40
    [Google Scholar]
  24. Strohl W. A., Rouse H., Teets K., Schlesinger R. W. 1970; The response of BHK21 cells to infection with type 12 adenovirus. III. Transformation and restricted replication of superinfecting type 2 adenovirus. Archiv für die gesamte Virusforschung 31:93–112
    [Google Scholar]
  25. Sutter D., Doerfler W. 1979; Methylation of integrated viral DNA sequences in hamster cells transformed by adenovirus type 12. Cold Spring Harbor Symposia on Quantitative Biology 44:565–568
    [Google Scholar]
  26. Sutter D., Doerfler W. 1980; Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression. Proceedings of the National Academy of Sciences of the United States of America 77:253–256
    [Google Scholar]
  27. Sutter D., Westphal M., Doerfler W. 1978; Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells. Cell 14:569–585
    [Google Scholar]
  28. van Der Eb A. J., van Ormondt H., Schrier L. P., Lupker J. H., Jochemsen H., van Den Elsen P. J., Deleys D. J., Maat L., Van Beveren C. P., Dijkema R., De Waard A. 1979; Structure and function of the transforming genes of human adenoviruses and SV40. Cold Spring Harbor Symposia on Quantitative Biology 44:383–399
    [Google Scholar]
  29. Vardimon L., Neumann R., Kuhlmann L., Sutter D., Doerfler W. 1980; Methylation of adenovirus DNA in transformed and infected cells. Nucleic Acids Research 8:2461–2473
    [Google Scholar]
  30. Vardimon L., Kuhlmann I., Doerfler W., Cedar H. 1981; Methylation of adenovirus genes in transformed cells and in vitro: influence on the regulation of gene expression?. European Journal of Cell Biology 25:13–15
    [Google Scholar]
  31. Vardimon L., Kressmann A., Cedar H., Maechler M., Doerfler W. 1982; Expression of a cloned adenovirus gene is inhibited by in vitro methylation. Proceedings of the National Academy of Sciences of the United States of America 79: (in press)
    [Google Scholar]
  32. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  33. Wold W. S. M., Chinnadurai G., Green M., Mak S. 1979; Identification of adenovirus type 12 candidate transformation proteins by radioimmunoprecipitation with antisera to EcoRI-C fragment transformed cells. Virology 94:208–213
    [Google Scholar]
  34. Zur Hausen H. 1973; Interactions of adenovirus type 12 with host cell chromosomes. Progress in Experimental Tumor Research 18:240–259
    [Google Scholar]
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