1887

Abstract

SUMMARY

Cultured cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 antigen in 0.1 to 1.0% of the cells. Control cultures infected with dengue-3 at the same multiplicity contained dengue-3 antigen in greater than 90% of the cells. The resistance to superinfection was not interferon-mediated, and occurred within 20 h after primary infection. In cultures simultaneously infected with two dengue virus types, one virus type was excluded from replication in most cells. A small population of cells was also found (about 1%) that contained type-specific antigen of both dengue virus types.

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1982-04-01
2024-11-05
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References

  1. Altman R. M. 1963; The behavior of Murray Valley encephalitis virus in Culex tritaeniorhynchus Giles and Curlex pipiens quinquefasciatus Say. American Journal of Tropical Medicine and Hygiene 12:425–434
    [Google Scholar]
  2. Bancroft W. H., Mccown J. M., Mas Laoo P., Brandt W. E., Russell P. K. 1979; Identification of dengue viruses from the Caribbean by plaque-reduction neutralization test. In Dengue in the Caribbean, 1977 pp 173–178 Pan American Health Organization (PAHO) publication; No 375 Washington D.C.:
    [Google Scholar]
  3. Cardiff R. D., Brandt W. E., Mccown T. G., Shapiro D., Russell P. K. 1971; Immunological and biophysical separation of dengue-2 antigens. Journal of Virology 7:15–23
    [Google Scholar]
  4. Chamberlain R. W., Sudia W. D. 1957; Dual infections of Eastern and Western-equine encephalitis viruses in Culex tarsalis. Journal of Infectious Diseases 101:233–236
    [Google Scholar]
  5. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  6. Davey M. W., Delgarno L. 1974; Semliki Forest virus replication in cultured Aedes albopictus cells: studies on the establishment of persistence. Journal of General Virology 24:453–463
    [Google Scholar]
  7. Davey M. W., Mahon R. J., Gibbs A. J. 1979; Togavirus interference in Culex annulirostris mosquitoes. Journal of General Virology 42:641–643
    [Google Scholar]
  8. Dittmar D. L., Haines H. G., Castro A. 1980; Monoclonal antibodies specific for dengue virus type 3. Journal of Clinical Microbiology 12:74–78
    [Google Scholar]
  9. Eaton B. T. 1979; Heterologous interference in Aedes albopictus cells infected with alphaviruses. Journal of Virology 30:45–55
    [Google Scholar]
  10. Gliedman J. B., Smith J. F., Brown D. T. 1975; Morphogenesis of Sindbis virus in cultured Aedes albopictus cells. Journal of Virology 16:913–926
    [Google Scholar]
  11. Hammon W. Mcd. 1973; Dengue hemorrhagic fever - Do we know its cause?. American Journal of Tropical Medicine and Hygiene 22:82–91
    [Google Scholar]
  12. Hunt J. M., Marcus P. I. 1974; Mechanism of Sindbis virus-induced intrinsic interference with vesicular stomatitis virus replication. Journal of Virology 14:99–109
    [Google Scholar]
  13. Igarashi A. 1979; Characteristics of Aedes albopictus cells persistently infected with dengue virus. Nature, London 280:690–691
    [Google Scholar]
  14. Igarashi A., Mantani M. 1974; Rapid titration of dengue virus type-4 infectivity by counting fluorescent foci. Biken Journal 17:87–93
    [Google Scholar]
  15. Kascsak R. J., Lyons M. J. 1974; Attempts to demonstrate the interferon defense mechanism in cultured mosquito cells. Archiv für die gesamte Virusforschung 45:149–154
    [Google Scholar]
  16. Lam K. S. K., Marshall I. D. 1968; Dual infections of Aedes aegypti with arboviruses. I. Arboviruses that have no apparent cytopathic effect in the mosquito. American Journal of Tropical Medicine and Hygiene 17:625–636
    [Google Scholar]
  17. Lechago J., Sun N. C. J., Weinstein W. M. 1979; Simultaneous visualization of two antigens in the same tissue section by combining immunoperoxidase with immunofluorescence techniques. Journal of Histochemistry and Cytochemistry 27:1221–1225
    [Google Scholar]
  18. Legault D., Takeyesu D., Prevec L. 1977; Heterotypic exclusion between vesicular stomatitis virus of the New Jersey and Indiana serotypes. Journal of General Virology 35:53–65
    [Google Scholar]
  19. Marcus P. I., Carver D. H. 1967; Intrinsic interference — a new type of viral interference. Journal of Virology 1:334–343
    [Google Scholar]
  20. Murphy F. A. 1975; Cellular resistance to arbovirus infection. Annals of the New York Academy of Sciences 266:197–203
    [Google Scholar]
  21. Murray A. M., Morahan P. S. 1973; Studies of interferon production in Aedes albopictus mosquito cells. Proceedings of the Society for Experimental Biology and Medicine 142:11–15
    [Google Scholar]
  22. Nimmannitya S., Halstead S. B., Cohen S. N., Margiotta M. R. 1969; Dengue and chikungunya virus infection in man in Thailand, 1962–1964. I. Observations on hospitalized patients with hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 18:954–971
    [Google Scholar]
  23. Peleg J. 1969; Inapparent persistent virus infection in continuously grown Aedes aegypti mosquito cells. Journal of General Virology 5:463–471
    [Google Scholar]
  24. Riedel B., Brown D. T. 1977; Role of extracellular virus in the maintenance of the persistent infection induced in Aedes albopictus (mosquito) cells by Sindbis virus. Journal of Virology 23:554–561
    [Google Scholar]
  25. Riedel B., Brown D. T. 1979; Novel antiviral activity found in the media of Sindbis virus persistently infected mosquito (Aedes albopictus) cell cultures. Journal of Virology 29:51–60
    [Google Scholar]
  26. Sabin A. B. 1952; Research on dengue during World War II. American Journal of Tropical Medicine and Hygiene 1:30–50
    [Google Scholar]
  27. Stollar V., Shenk T. E. 1973; Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. Journal of Virology 11:592–595
    [Google Scholar]
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