1887

Abstract

SUMMARY

Simian sarcoma-associated virus (SSaV) was repeatedly passaged on three human cell lines. The proteins of the progeny virus were analysed for the presence of variant polypeptides. Occasionally, a few variant polypeptides were observed. One-dimensional peptide maps of the major virus protein p30 revealed no modifications after 25 cycles of infection on the three cell lines studied. The peptide map of Pr65 of virus grown through 25 passages on a human chondrosarcoma cell line was slightly different from that of the virus stock before passaging. The relative amount of the virus protein p30 as compared to p18 and p16 (possibly the SSaV equivalents of p15E and p12E) was variable depending on the host cell. Virus grown on Daudi cells was relatively deficient in p18 and p16. These virus particles were morphologically altered and had a low infectivity.

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1982-03-01
2024-04-27
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References

  1. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  2. De Man J. C. H., Persant Snoep M., Huiskens-van Der Meij J. W., Meera Khan P., Schaberg A., Warnaar S. O. 1977; A new cell line from a human chondrosarcoma. British Journal of Cancer 35:403–414
    [Google Scholar]
  3. Gallagher R. E., Gallo R. C. 1975; Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells. Science 187:350–353
    [Google Scholar]
  4. Gallo R. C., Wong-Staal F. 1980 In Viral Oncology, Molecular Biology of Primate Retroviruses pp 399–431 Edited by Klein G. New York: Raven Press;
    [Google Scholar]
  5. Herbrink P., Moen J. E. T., Brouwer J., Warnaar S. O. 1980; Detection of antibodies cross-reactive with type C RNA tumor viral p30 protein in human sera and exudate fluids. Cancer Research 40:166–173
    [Google Scholar]
  6. Karshin W. L., Arcement L. J., Naso R. B., Arkinghaus R. B. 1977; A common precursor fc• Rauscher leukemia virus gp69/71, p15 (E) and p12 (E). Journal of Virology 23:787–798
    [Google Scholar]
  7. Klein E., Klein G., Nadkarni J. S. 1967; Surface IgM specificity on cells derived from a Burkitt’s lymphoma. Lancet ii:1068
    [Google Scholar]
  8. Lu A. H., Soong M. M., Wong P. K. Y. 1979; Maturation of Moloney murine leukemia virus. Virology 93:269–274
    [Google Scholar]
  9. Maizel V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:178–198
    [Google Scholar]
  10. Mooren H. W. D., Prins F. A., Warnaar S. O. 1980; Time course of budding and maturation of R-MuLV-ts29 studied by electron microscopy. Virology 102:211–213
    [Google Scholar]
  11. Nooter K. J., Bentvelzen P., Zurcher C., Rhim J. 1977; Detection of human C type helper virus in human leukemic bone marrow with murine sarcoma virus-transformed human and rat non-producer cells. International Journal of Cancer 21:27–34
    [Google Scholar]
  12. Oroszlan S., Copeland T., Smythers G., Summers M. R., Gieder R. V. 1977; Comparative primary structure analysis of the p30 protein of woolly monkey and gibbon type C viruses. Virology 77:413–417
    [Google Scholar]
  13. Panem S., Prochownik E. V., Reale F. R., Kirsten W. H. 1975; Isolation of C type virions from a normal human fibroblast strain. Science 189:297–299
    [Google Scholar]
  14. Sahagan B. G., Haseltine W. A. 1979; Structural analysis of the genome of gibbon ape and woolly monkey leukosis viruses. Journal of Virology 31:657–667
    [Google Scholar]
  15. Sahagan B. G., Haseltine W. A. 1980; Relationship of retroviruses isolated from human leukemia tissues to the woolly monkey-gibbon ape leukemia viruses. Journal of Virology 34:390–401
    [Google Scholar]
  16. Schindler J., Hynes R., Hopkins N. 1977; Evidence for recombination between N and B tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulphate-polyacryl amide gel electrophoresis. Journal of Virology 23:700–707
    [Google Scholar]
  17. Van Muijen G. N. P., Velde J. Te., Ottolander G. J. Den., Brand A., Koopman-Broekhuijzen N., Schaberg A., Warnaar S. O. 1979; On the presence of reverse transcriptase in myelo- and lymphoproliferative disorders. Cancer 43:1682–1688
    [Google Scholar]
  18. Wolfe L. G., Deinhardt F., Theilen G. H., Rabin H., Kawakami T., Bustad L. K. 1971; Induction of tumors in marmoset monkeys by simian sarcoma virus type 1 (Lagothrix): a preliminary report. Journal of the National Cancer Institute 47:1115–1120
    [Google Scholar]
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