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Journal of General Virology header logo Journal of General Virology

Volume 59, Issue 1

Characterization of the Hallé SSPE Measles Virus Isolate No Access

Abstract

SUMMARY

The Hallé subacute sclerosing panencephalitis (SSPE) measles virus isolate and its plaque-purified progeny were investigated to determine whether any unusual properties could be associated with its ability to cause persistent infection. Three types of plaque-purified progeny were isolated. One population appeared to be similar in biological and biochemical properties to laboratory-adapted measles virus and had the ability to induce syncytia (syn). A second population (syn) plaqued more efficiently at 39 °C than at 33 °C, did not cause normal cell fusion at either temperature, and produced particles that interfered with the replication of other measles virus isolates and . This syn virus was further plaquepurified to eliminate the interfering particles, producing the syn P2 virus. This virus also plaqued more efficiently at 39 °C than at 33 °C, but caused cell fusion only at 39 °C. Both syn viruses and the parental virus were significantly less virulent than the syn virus and caused a more prolonged infection. Biochemical analysis showed that the syn P2 population produced particles that banded at two different densities in potassium tartrate gradients; both densities were less than those of the standard laboratory measles virus and the syn virus. Although the syn P2 virus did not cause cell fusion at 33 °C, [S]methionine labelling demonstrated that the haemolysin/cell fusion protein was present in syn P2 virions. The production of interfering particles, the inability to cause cell fusion at 33 °C, and the cold-sensitive nature of the syn population appear to play a role in the ability of the Hallé SSPE virus to establish persistent infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Hallé SSPE variants , measles virus isolate and persistent infection
© Journal of General Virology 1982
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1982-03-01
2025-01-18
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/content/journal/jgv/10.1099/0022-1317-59-1-57
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Characterization of the Hallé SSPE Measles Virus Isolate
J. Gen. Virol. 59, 57 (1982); https://doi.org/10.1099/0022-1317-59-1-57
/content/journal/jgv/10.1099/0022-1317-59-1-57
/content/journal/jgv/10.1099/0022-1317-59-1-57
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