1887

Abstract

SUMMARY

The Hallé subacute sclerosing panencephalitis (SSPE) measles virus isolate and its plaque-purified progeny were investigated to determine whether any unusual properties could be associated with its ability to cause persistent infection. Three types of plaque-purified progeny were isolated. One population appeared to be similar in biological and biochemical properties to laboratory-adapted measles virus and had the ability to induce syncytia (syn). A second population (syn) plaqued more efficiently at 39 °C than at 33 °C, did not cause normal cell fusion at either temperature, and produced particles that interfered with the replication of other measles virus isolates and . This syn virus was further plaquepurified to eliminate the interfering particles, producing the syn P2 virus. This virus also plaqued more efficiently at 39 °C than at 33 °C, but caused cell fusion only at 39 °C. Both syn viruses and the parental virus were significantly less virulent than the syn virus and caused a more prolonged infection. Biochemical analysis showed that the syn P2 population produced particles that banded at two different densities in potassium tartrate gradients; both densities were less than those of the standard laboratory measles virus and the syn virus. Although the syn P2 virus did not cause cell fusion at 33 °C, [S]methionine labelling demonstrated that the haemolysin/cell fusion protein was present in syn P2 virions. The production of interfering particles, the inability to cause cell fusion at 33 °C, and the cold-sensitive nature of the syn population appear to play a role in the ability of the Hallé SSPE virus to establish persistent infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-59-1-57
1982-03-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/59/1/JV0590010057.html?itemId=/content/journal/jgv/10.1099/0022-1317-59-1-57&mimeType=html&fmt=ahah

References

  1. Breschkin A. M., Walmer B., Rapp F. 1977; Hemagglutination variant of measles virus. Virology 80:441–444
    [Google Scholar]
  2. Ecob-Johnston M. S., Bornstein M. B., Raine C. S. 1977; Long-term infection of cultured hamster dorsal root ganglia with Hallé SSPE virus. Neuropathology and Applied Neurobiology 3:267–279
    [Google Scholar]
  3. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–185
    [Google Scholar]
  4. Hall W. W., Ter Meulen V. 1976; RNA homology between subacute sclerosing panencephalitis and measles virus. Nature, London 264:474–477
    [Google Scholar]
  5. Hall W. W., Martin S. J., Gould E. A. 1974; Defective interfering particles produced during the replication of measles virus. Medical Microbiology and Immunology 160:155–164
    [Google Scholar]
  6. Hall W. W., Kiessling W., Ter Meulen V. 1978; Membrane proteins of subacute sclerosing panencephalitis and measles virus. Nature, London 272:460–462
    [Google Scholar]
  7. Hall W. W., Lamb R. A., Choppin P. W. 1979; Measles and subacute sclerosing panencephalitis virus proteins: lack of antibodies to the M protein in patients with subacute sclerosing panencephalitis. Proceedings of the National Academy of Sciences of the United States of America 76:2047–2051
    [Google Scholar]
  8. Hamilton R., Barbosa L., Dubois M. 1973; Subacute sclerosing panencephalitis measles virus: study of biological markers. Journal of Virology 12:632–642
    [Google Scholar]
  9. Haspel M. V., Knight P. R., Duff R. G., Rapp F. 1973; Activation of a latent measles virus infection in hamster cells. Journal of Virology 12:690–695
    [Google Scholar]
  10. Horta-Barbosa L., Hamilton R., Wittig B., Fucillo D., Sever J. L. 1971a; Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science 173:840–841
    [Google Scholar]
  11. Horta-Barbosa L., Krebs H., Ley A., Chen T. C., Gilkeson M. R., Sever J. L. 1971b; Progressive increase in cerebrospinal fluid measles antibody levels in subacute sclerosing panencephalitis. Pediatrics 47:782–783
    [Google Scholar]
  12. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  13. Joseph B. S., Cooper N. R., Oldstone M. B. A. 1975; Immunologic injury of cultured cells infected with measles virus. I. Role of IgG antibody and the alternative complement pathway. Journal of Experimental Medicine 141:761–774
    [Google Scholar]
  14. Kiley M. P., Payne F. E. 1974; Evidence of precursors of defective measles virus. Medical Microbiology and Immunology 160:91–97
    [Google Scholar]
  15. Kratzsch V., Hall W. W., Nagashima K., Ter Meulen V. 1977; Biological and biochemical characterization of a latent subacute sclerosing panencephalitis (SSPE) virus infection in tissue culture. Journal of Medical Virology 1:139–154
    [Google Scholar]
  16. Lucas A., Coulter M., Anderson R., Dales S., Flintoff W. 1978; In vivo and in vitro models of demyelinating diseases. II. Persistence and host-regulated thermosensitivity in cells of neural derivation infected with mouse hepatitis and measles viruses. Virology 88:325–337
    [Google Scholar]
  17. McKlmm J., Rapp F. 1977a; Inability of measles virus temperature-sensitive mutants to induce interferon. Virology 76:409–415
    [Google Scholar]
  18. McKlmm J., Rapp F. 1977b; Variation in ability of measles virus plaque progeny to induce interferon. Proceedings of the National Academy of Sciences of the United States of America 74:3056–3059
    [Google Scholar]
  19. Minagawa T., Sakuma T., Kuwajima S., Yamamoto T. K., Hda H. 1976; Characterization of measles viruses in establishment of persistent infections in human lymphoid cell line. Journal of General Virology 33:361–379
    [Google Scholar]
  20. Morgan E. M., Rapp F. 1977; Measles virus and its associated diseases. Bacteriological Reviews 41:636–666
    [Google Scholar]
  21. Rima B. K., Martin S. J., Gould E. M. 1979; A comparison of polypeptides in measles and SSPE strains. Journal of General Virology 42:603–608
    [Google Scholar]
  22. Wechsler S. L., Fields B. N. 1978; Differences between the intracellular polypeptides of measles and subacute sclerosing panencephalitis virus. Nature, London 272:458–460
    [Google Scholar]
  23. Wild T. F., Dugre R. 1978; Establishment and characterization of a subacute sclerosing panencephalitis (measles) virus persistent infection in BGM cells. Journal of General Virology 39:113–124
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-59-1-57
Loading
/content/journal/jgv/10.1099/0022-1317-59-1-57
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error