1887

Abstract

SUMMARY

Potato spindle tuber viroid (PSTV) RNA, labelled with I, was hybridized in solution to RNA prepared from uninfected and PSTV-infected Rutgers tomato plants and suspension cultures. Following hybridization to RNA from infected plants, I-labelled PSTV was converted from its single-stranded form to double-stranded RNA; this conversion did not occur to a significant extent when the I-labelled PSTV was incubated with RNA from uninfected tomato plants under identical conditions. Following fractionation of RNA from PSTV-infected tissue with 2 -LiCl and chromatography on cellulose CF11 columns, the RNA species which hybridizes with the PSTV probe was found to be enriched in those fractions which are also enriched for double-stranded RNA. Fingerprint analysis of hybridized I-labelled PSTV following recovery from the hybrids demonstrated that all regions of the viroid are represented in the complementary strands present in these RNA preparations.

Keyword(s): complementary RNA , PSTV and viroid
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-59-1-139
1982-03-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/59/1/JV0590010139.html?itemId=/content/journal/jgv/10.1099/0022-1317-59-1-139&mimeType=html&fmt=ahah

References

  1. Bellamy A. R., Ralph R. K. 1968; Recovery and purification of nucleic acids by means of cetyltrimethylammonium bromide. Methods in Enzymology 12B:156–169
    [Google Scholar]
  2. Branch A. D., Dickson E. 1980; Tomato DNA contains no detectable regions complementary to potato spindle tuber viroid as assayed by Southern hybridization. Virology 104:10–26
    [Google Scholar]
  3. Branch A. D., Robertson H. D., Dickson E. 1981; Longer than unit length viroid minus strands are present in RNA from infected plants. Proceedings of the National Academy of Sciences of the United States of America (in press)
    [Google Scholar]
  4. Caspar D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Advances in Protein Chemistry 18:37–21
    [Google Scholar]
  5. Dickson E. 1979; Viroids: infectious RNA in plants. In Nucleic Acids in Plants pp 153–193 Edited by Hall T. C., Davies J. W. Florida: CRC Press;
    [Google Scholar]
  6. Dickson E., Pape L. K., Robertson H. D. 1979a; Approaches to sequence analysis of 125I-labelled RNA. Nucleic Acids Research 6:91–110
    [Google Scholar]
  7. Dickson E., Robertson H. D., Niblett C. L., Horst R. K., Zaitlin M. 1979b; Minor differences between nucleotide sequences of mild and severe strains of potato spindle tuber viroid. Nature, London 277:60–62
    [Google Scholar]
  8. Diener T. O. 1972; Potato spindle tuber viroid. VIII. Correlation of infectivity with a UV-absorbing component and thermal denaturation properties of the RNA. Virology 50:606–609
    [Google Scholar]
  9. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences of the United States of America 55:1504–1511
    [Google Scholar]
  10. Grill L. K., Semancik J. S. 1978; RNA sequences complementary to citrus exocortis viroid in nucleic acid preparations from infected Gynura aurantiaca. Proceedings of the National Academy of Sciences of the United States of America 75:896–900
    [Google Scholar]
  11. Grill L. K., Negruk V. I., Semancik J. S. 1980; Properties of the complementary RNA sequences associated with infection by the citrus exocortis viroid. Virology 107:24–33
    [Google Scholar]
  12. Gross H. J., Domdey H., Lossow C., Jank P., Raba M., Alberty H., Sӓnger H. L. 1978; Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature, London 273:203–208
    [Google Scholar]
  13. Jeppesen P. G. N. 1971; The nucleotide sequences of some large ribonuclease T1 products from bacteriophage R17 ribonucleic acid. Biochemical Journal 124:357–366
    [Google Scholar]
  14. Owens R. A., Cress D. E. 1980; Molecular cloning and characterization of potato spindle tuber viroid cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 77:5302–5306
    [Google Scholar]
  15. Rackwitz H. R., Rohde W., Sӓnger H. L. 1981; DNA-dependent RNA polymerase II of plant origin transcribes viroid RNA into full-length copies. Nature, London 291:297–301
    [Google Scholar]
  16. Robertson H. D., Dunn J. J. 1975; Ribonucleic acid processing activity of Escherichia coli ribonuclease III. Journal of Biological Chemistry 250:3050–3056
    [Google Scholar]
  17. Robertson H. D., Hunter T. 1975; Sensitive methods for the detection and characterization of double helical ribonucleic acid. Journal of Biological Chemistry 250:418–425
    [Google Scholar]
  18. Robertson H. D., Webster R. E., Zinder N. D. 1968; Purification and properties of ribonuclease III from Escherichia coli. Journal of Biological Chemistry 243:82–91
    [Google Scholar]
  19. Zaitlin M., Duda C. T., Petti M. A. 1973; Replication of tobacco mosaic virus. V. Properties of the bound and solubilized replicase. Virology 53:300–311
    [Google Scholar]
  20. Zajtlin M., Niblett C. L., Dickson E., Goldberg R. B. 1980; Tomato DNA contains no detectable regions complementary to potato spindle tuber viroid as assayed by solution and filter hybridization. Virology 104 :1–9
    [Google Scholar]
  21. Zelcer A., van Adelsberg J., Leonard D. A., Zaitlin M. 1981; Plant cell suspensions sustain long-term replication of potato spindle tuber viroid. Virology 109:314–322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-59-1-139
Loading
/content/journal/jgv/10.1099/0022-1317-59-1-139
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error